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Characterization of ginsenoside extracts by delayed luminescence, high-performance liquid chromatography, and bioactivity tests

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Abstract

Ginsenoside extracts are often used as raw materials for various pharmaceutical, cosmetic and food supplement products. Development of a direct, rapid, cheap, and comprehensive measurement tool for the quality assessment of ginsenoside extracts, and indeed all herbal extracts, is urgently needed. In addition, a bioactivity-based assessment should be linked with quality control. In this report, we try to develop a novel quality control tool using ginsenoside extracts as an example. High-performance liquid chromatography (HPLC) was used to detect nine principal ginsenosides in 11 batches of ginsenoside extracts. Delayed luminescence (DL) was used to analyze the same ginsenoside extract samples. DL measurements showed the same results in terms of differentiating 11 ginsenoside extract samples compared with chemical analysis, and DL properties could be closely linked to index ginsenosides in the quality control of ginsenoside extracts. Next, a zebrafish tail-fin amputation model was used to study differences in antiinflammatory effect between the ginsenoside extract batches. The results indicate that both chemical analysis and DL measurements could partially reflect biological activity. Thus, DL may serve as a rapid, direct, sensitive, and systemic tool for studying the overall properties of ginsenoside extracts. Our proposal for linking bioactivities as a tool for evaluation of the quality of ginsenoside extracts opens a new direction for quality control.

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Authors and Affiliations

  1. Leiden University-European Center for Chinese Medicine and Natural Compounds, Institute of Biology, Leiden University, Sylviusweg 72, 2333BE, Leiden, The Netherlands

    Mengmeng Sun, Min He, Mahmoud Halima & Mei Wang

  2. Changchun University of Chinese Medicine, No. 1035, Boshuo Rd, Jingyue Economic Development District, 130117, Changchun, China

    Mengmeng Sun

  3. Fytagoras BV, Sylviusweg 72, 2333BE, The Netherlands

    Henrie Korthout

  4. Plant Science and Natural Products, Institute of Biology Leiden, Leiden University, Sylviusweg 72, 2333BE, Leiden, The Netherlands

    Hye Kyong Kim

  5. Meluna Research, Koppelsedijk 1-a, 4191LC, Geldermalsen, The Netherlands

    Yu Yan, Eduard van Wijk & Roeland van Wijk

  6. Sino-Dutch Centre for Preventive and Personalized Medicine, Gasthuislingelaan 33, 4002AG, Tiel, The Netherlands

    Yu Yan, Eduard van Wijk & Roeland van Wijk

  7. Jilin ZiXin Pharmaceutical Co., Ltd, No. 137, Dongtou-daojie Rd, Nanguan District, 130041, Changchun, China

    Chunsheng Guo

  8. SU BioMedicine, Postbus 546, 2300AM, Leiden, The Netherlands

    Mei Wang

Authors
  1. Mengmeng Sun
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  2. Min He
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  3. Henrie Korthout
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  4. Mahmoud Halima
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  5. Hye Kyong Kim
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  6. Yu Yan
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  7. Eduard van Wijk
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  8. Roeland van Wijk
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  9. Chunsheng Guo
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  10. Mei Wang
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Corresponding author

Correspondence to Mei Wang.

Additional information

Electronic supplementary information (ESI) available. See DOI: 10.1039/c8pp00533h

These two authors contributed equally to this work.

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Sun, M., He, M., Korthout, H. et al. Characterization of ginsenoside extracts by delayed luminescence, high-performance liquid chromatography, and bioactivity tests. Photochem Photobiol Sci 18, 1138–1146 (2019). https://doi.org/10.1039/c8pp00533h

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  • DOI: https://doi.org/10.1039/c8pp00533h

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