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Irradiation with 310 nm and 340 nm ultraviolet light-emitting-diodes can improve atopic dermatitis-like skin lesions in NC/Nga mice

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Abstract

Ultraviolet (UV) light produces an immunomodulatory effect on the skin and is widely used for the treatment of chronic inflammatory skin diseases. UV light emitting diodes (UV-LEDs) are a new and promising source of UV radiation. However, their mechanism of action remains largely unknown. In this study, we tested the safety and effectiveness of UV-LED irradiation for the treatment of atopic dermatitis (AD) in an NC/Nga mouse model. Mice were divided into seven groups of eight mice each. Application of Dermatophagoides faunae (Df) extract ointment for four weeks induced AD-like skin lesions. Subsequently, the mice were exposed to UV-LEDs, narrow band UVB, or UVA irradiation three times per week. We assessed the immunosuppressive effects of 310 nm (50 mJ cm−2) and 340 nm (5 J cm−2) UV-LED irradiation. Histological analyses using hematoxylin-eosin, toluidine blue, and immunohisto-chemical staining were performed. In addition, the serum levels of IgE, inflammatory cytokines and chemokines were measured using enzyme-linked immunosorbent assays (ELISAs). UV-LED irradiation significantly alleviated AD-like skin symptoms, including edema, erythema, dryness, and itching, by modulating Th1 and Th2 responses, transepidermal water loss (TEWL), and scratching behavior in NC/Nga mice. These results suggest that UV-LEDs can improve the treatment of inflammatory skin diseases.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A1B03030435).

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Authors and Affiliations

  1. Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea

    Tae-Rin Kwon, Jong Hwan Kim, Ji-Yeon Hong, Joon Seok, Jae Min Kim, Dong-Ho Bak, Mi-Ji Choi & Beom Joon Kim

  2. Department of Medicine, Graduate School, Chung-Ang University, Seoul, Korea

    Jong Hwan Kim, Ji-Yeon Hong, Joon Seok, Jae Min Kim, Dong-Ho Bak, Mi-Ji Choi & Beom Joon Kim

  3. Department of Otorhinolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, Seoul, Korea

    Seok Kyun Mun

  4. Department of Emergency Medicine, Chung-Ang University College of Medicine, Seoul, Korea

    Chan Woong Kim

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  1. Tae-Rin Kwon
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Correspondence to Beom Joon Kim.

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Kwon, TR., Kim, J.H., Hong, JY. et al. Irradiation with 310 nm and 340 nm ultraviolet light-emitting-diodes can improve atopic dermatitis-like skin lesions in NC/Nga mice. Photochem Photobiol Sci 17, 1127–1135 (2018). https://doi.org/10.1039/c8pp00063h

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