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Heat shock antagonizes UVA-induced responses in murine melanocytes and melanoma cells: an unexpected interaction

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Abstract

The skin is under the influence of oscillatory factors such as light and temperature. This organ possesses a local system that controls several aspects in a time-dependent manner; moreover, the skin has a well-known set of opsins whose function is still unknown. We demonstrate that heat shock reduces Opn2 expression in normal Melan-a melanocytes, while the opposite effect is found in malignant B16-F10 cells. In both cell lines, UVA. radiation increases the expression of Opn4 and melanin content. Clock genes and Xpa, a DNA. repair gene, of malignant melanocytes are more responsive to UVA. radiation when compared to normal cells. Most UVA-induced effects are antagonized by heat shock, a phenomenon shown for the first time. Based on our data, the heat produced during UV. experiments should be carefully monitored since temperature represents, according to our results, an important confounding factor, and therefore it should, when possible, be dissociated from UV. radiation. The responses displayed by murine melanoma cells, if proven to also take place in human melanoma, may represent an important step in cancer development and progression

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Abbreviations

6-4 PP:

6,4 Photoproducts

Aqp3:

Aquaporin 3 gene

BMAL1:

Aryl hydrocarbon receptor nuclear translocator- like protein 1

Bmal1:

Aryl hydrocarbon receptor nuclear translocatorlike protein 1 gene

CLOCK.:

Circadian locomotor output cycles kaput protein

Clock:

Circadian locomotor output cycles kaput gene

CPD:

Cyclobutane pyrimidine dimers

CRY:

Cryptochrome protein

Cry:

Cryptochrome gene

DD:

Constant dark

FBS:

Fetal bovine serum

IPD:

Immediate pigment darkening

LD:

Light-dark cycle

PER:

Period protein

Per:

Period gene

PI:

Propidium iodide

Rev-Erba/β:

RAR-related orphan receptor alpha/beta gene RORalβ Nuclear receptor subfamily 1, group D, member 1/2 gene

SCNs:

Suprachiasmatic nuclei

TPA:

Phorbol 12-myristate 13-acetate

UVB:

Ultraviolet B radiation

XPA:

Xeroderma pigmentosum, complementation group A protein

Xpa:

Xeroderma pigmentosum, complementation group A. gene

ZT:

Zeitgeber time

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  1. Laboratory of Comparative Physiology of Pigmentation, Department of Physiology, Institute of Biosciences, University of Sao Paulo, Sao Paulo, Brazil

    Leonardo Vinícius Monteiro de Assis, Maria Nathália Moraes & Ana Maria de Lauro Castrucci

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  1. Leonardo Vinícius Monteiro de Assis
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  2. Maria Nathália Moraes
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de Assis, L.V.M., Moraes, M.N. & de Lauro Castrucci, A.M. Heat shock antagonizes UVA-induced responses in murine melanocytes and melanoma cells: an unexpected interaction. Photochem Photobiol Sci 16, 633–648 (2017). https://doi.org/10.1039/c6pp00330c

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  • DOI: https://doi.org/10.1039/c6pp00330c

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