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A ruthenium(ii) based photosensitizer and transferrin complexes enhance photo-physical properties, cell uptake, and photodynamic therapy safety and efficacy

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Abstract

Metal-based photosensitizers are of interest as their absorption and chemical binding properties can be modified via the use of different ligands. Ru2+ based photosensitizers are known to be effective photodynamic therapy (PDT) agents against bacteria, whereas use for oncological indications in vivo has not been demonstrated with the same level of evidence. We present data showing that premixing the Ru2+-complex TLD1433 with transferrin increases the molar extinction coefficient, including longer activation wavelengths, reduces photobleaching rates, and reduces the toxicity of the complex improving overall PDT efficacy. As the transferrin receptor is upregulated in most malignancies, premixing the Ru2+ complex with transferrin converts the active pharmaceutical ingredient TLD1433 into a drug of potentially considerable clinical utility.

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Authors and Affiliations

  1. Theralase Inc., 1945 Queen Street East, Toronto, ON, M4L1H7, Canada

    Pavel Kaspler, Savo Lazic, Yaxal Arenas & Arkady Mandel

  2. Department of Medical Biophysics, University Health Network, Princess Margaret Cancer Research Tower and University of Toronto, 101 College Street, Toronto, ON, M5G1L7, Canada

    Sarah Forward & Lothar Lilge

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  1. Pavel Kaspler
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  2. Savo Lazic
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Correspondence to Lothar Lilge.

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Kaspler, P., Lazic, S., Forward, S. et al. A ruthenium(ii) based photosensitizer and transferrin complexes enhance photo-physical properties, cell uptake, and photodynamic therapy safety and efficacy. Photochem Photobiol Sci 15, 481–495 (2016). https://doi.org/10.1039/c5pp00450k

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