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Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases

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Abstract

Numerous proteases are known to be necessary for cancer development and progression including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins. The goal of this research is to develop an Fe/Fe3O4 nanoparticle-based system for clinical diagnostics, which has the potential to measure the activity of cancer-associated proteases in biospecimens. Nanoparticle-based “light switches” for measuring protease activity consist of fluorescent cyanine dyes and porphyrins that are attached to Fe/Fe3O4 nanoparticles via consensus sequences. These consensus sequences can be cleaved in the presence of the correct protease, thus releasing a fluorescent dye from the Fe/Fe3O4 nanoparticle, resulting in highly sensitive (down to 1 × 10−16 mol l−1 for 12 proteases), selective, and fast nanoplatforms (required time: 60 min).

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Authors and Affiliations

  1. Kansas State University, Department of Chemistry, 213 CBC Building, Manhattan, KS, USA

    Hongwang Wang, Dinusha N. Udukala, Thilani N. Samarakoon, Gayani Abayaweera, Harshi Manawadu, Aruni Malalasekera, Colette Robinson, David Villanueva, Pamela Maynez, Leonie Bossmann, Elizabeth Riedy, Jenny Barriga, Ni Wang, Ping Li, Daniel A. Higgins & Stefan H. Bossmann

  2. Department of Anatomy & Physiology, Kansas State University, 130 Coles Hall, Manhattan, KS, USA

    Matthew T. Basel & Deryl L. Troyer

  3. University of Utah School of Medicine, 201 South Presidents Circle, Salt Lake City, UT, 84112, USA

    Mausam Kalita

  4. Department of Nuclear Medicine, The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming, Yunnan, China

    Gaohong Zhu

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  1. Hongwang Wang
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  2. Dinusha N. Udukala
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Corresponding authors

Correspondence to Hongwang Wang, Dinusha N. Udukala or Stefan H. Bossmann.

Additional information

Electronic supplementary information (ESI) available: Synthesis and characterization of the nanoplatform and its components, as well as additional photophysical information. See DOI: 10.1039/c3pp50260k

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Wang, H., Udukala, D.N., Samarakoon, T.N. et al. Nanoplatforms for highly sensitive fluorescence detection of cancer-related proteases. Photochem Photobiol Sci 13, 231–240 (2014). https://doi.org/10.1039/c3pp50260k

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