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Growth conditions influence UVB sensitivity and oxidative damage in an estuarine bacterial isolate

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Abstract

The dose-dependent variation of oxidative cellular damage imposed by UVB exposure in a representative estuarine bacterial strain, Pseudomonas sp. NT5I1.2B, was studied at different growth phases (mid-exponential, late-exponential, and stationary), growth temperatures (15 °C and 25 °C) and growth media (nutrient-rich Tryptic Soy Broth [TSB] and nutrient-poor M9). Survival and markers of oxidative damage (lipid peroxidation, proteincarbonylation, DNA strand breakage, and DNA–protein cross-links) were monitored during exposure to increasing UVB doses (0–60 kJ m-2). Oxidative damage did not follow a clear linear dose-dependent pattern, particularly at high UVB doses (>10 kJ m-2), suggesting a dynamic interaction between damage induction and repair during irradiation and/or saturation of oxidative damage. Survival of stationary phase cells generally exceeded that of exponential phase cells by up to 33.5 times; the latter displayed enhanced levels of DNA—protein cross-links (up to 15.6-fold) and proteincarbonylation (up to 6.0-fold). Survival of mid-exponential phase cells was generally higher at 15 °C than at 25 °C (up to 6.6-fold), which was accompanied by lower levels of DNA strand breaks (up to 4000-fold), suggesting a temperature effect on reactive oxygen species (ROS) generation and/or ROS interaction with cellular targets. Survival under medium–high UVB doses (>10 kJ m-2) was generally higher (up to 5.4-fold) in cells grown in TSB than in M9. These results highlight the influence of growth conditions preceding irradiation on the extent of oxidative damage induced by UVB exposure in bacteria.

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  1. Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    Ana L. Santos, Newton C. M. Gomes, Isabel Henriques, Adelaide Almeida, António Correia & Ângela Cunha

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Santos, A.L., Gomes, N.C.M., Henriques, I. et al. Growth conditions influence UVB sensitivity and oxidative damage in an estuarine bacterial isolate. Photochem Photobiol Sci 12, 974–986 (2013). https://doi.org/10.1039/c3pp25353h

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