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PsbS-specific zeaxanthin-independent changes in fluorescence emission spectrum as a signature of energy-dependent non-photochemical quenching in higher plants

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Abstract

The PsbS protein of photosystem II is necessary for the development of energy-dependent quenching of chlorophyll (Chl) fluorescence (qE), and PsbS-deficient Arabidopsis plant leaves failed to show qE-specific changes in the steady-state 77 K fluorescence emission spectra observed in wild-type leaves. The difference spectrum between the quenched and un-quenched states showed a negative peak at 682 nm. Although the level of qE development in the zeaxanthin-less npq1-2 mutant plants, which lacked violaxanthin de-epoxidase enzyme, was only half that of wild type, there were no noticeable changes in this qE-dependent difference spectrum. This zeaxanthin-independent ΔF682 signal was not dependent on state transition, and the signal was not due to photobleaching of pigments either. These results suggest that ΔF682 signal is formed due to PsbS-specific conformational changes in the quenching site of qE and is a new signature of qE generation in higher plants.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Molecular Biology, Pusan National University, Busan, 609-735, Korea

    Ismayil S. Zulfugarov, Altanzaya Tovuu, Bolormaa Dogsom & Choon-Hwan Lee

  2. Institute of Botany, Azerbaijan National Academy of Sciences, Patamdar Shosse 40, Baku, AZ 1073, Azerbaijan

    Ismayil S. Zulfugarov

  3. Department of Plant Bioscience, Pusan National University, Miryang, 627-906, Korea

    Chung Yeol Lee

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  1. Ismayil S. Zulfugarov
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  2. Altanzaya Tovuu
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Correspondence to Choon-Hwan Lee.

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Electronic supplementary information (ESI) available: The 77 K difference fluorescence emission spectra (quenched state minus unquenched state) normalized at 750 nm; the slow chlorophyll fluorescence induction curves of the leaves; the 77 K un-normalized fluorescence emission spectra of thylakoids. See DOI: 10.1039/b9pp00132h

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Zulfugarov, I.S., Tovuu, A., Dogsom, B. et al. PsbS-specific zeaxanthin-independent changes in fluorescence emission spectrum as a signature of energy-dependent non-photochemical quenching in higher plants. Photochem Photobiol Sci 9, 697–703 (2010). https://doi.org/10.1039/b9pp00132h

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