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Identification of 3,4-didehydrorhodopin as major carotenoid in Rhodopseudomonas species

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Abstract

Recently we isolated the purple photosynthetic bacterium, Rhodopseudomonas sp. Rits, which was phylogenetically related to Rhodopseudomonas (Rps.) palustris. In this study, the light-dependent and time-dependent changes in the carotenoid composition were investigated by HPLC analysis of extracts from the cultures. All seven carotenoids in the biosynthetic pathway from lycopene to spirilloxanthin were detected. Especially, 3,4-didehydrorhodopin, having twelve conjugated double bonds as well as one terminal hydroxy group, was isolated in a remarkably large amount and fully characterized for the first time. The biosynthetic intermediate was commonly found in the Rps. palustris strains (CGA009, Morita and NBRC100419).

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga, 525-8577, Japan

    Tadashi Mizoguchi, Megumi Isaji, Jiro Harada & Hitoshi Tamiaki

Authors
  1. Tadashi Mizoguchi
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  2. Megumi Isaji
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  3. Jiro Harada
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  4. Hitoshi Tamiaki
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Corresponding author

Correspondence to Hitoshi Tamiaki.

Additional information

Electronic supplementary information (ESI) available: Details of the mass spectral analysis, electronic-absorption properties of each carotenoid found in Rhodopseudomonas sp. Rits and1H-NMR data of the isolated 3,4-didehydrorhodopin. See DOI: 10.1039/b719272j

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Mizoguchi, T., Isaji, M., Harada, J. et al. Identification of 3,4-didehydrorhodopin as major carotenoid in Rhodopseudomonas species. Photochem Photobiol Sci 7, 492–497 (2008). https://doi.org/10.1039/b719272j

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  • DOI: https://doi.org/10.1039/b719272j

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