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Single- and multi-photon excited fluorescence from serotonin complexed with ß-cyclodextrin

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A Correction to this article was published on 01 May 2007

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Abstract

The fluorescence of serotonin on binding with β-cyclodextrin has been studied using both steady state and time-resolved methods. Steady state fluorescence intensity of serotonin at 340 nm showed ~30% increase in intensity on binding with KA ~ 60 dm3 mol−1 and the fluorescence lifetimes showed a corresponding increase. In contrast, the characteristic green fluorescence (‘hyperluminescence’) of serotonin observed upon multiphoton near-infrared excitation with sub-picosecond pulses was resolved into two lifetime components assigned to free and bound serotonin. The results are of interest in relation to selective imaging and detection of serotonin using the unusual hyperluminescence emission and in respect to recent determinations of serotonin by capillary electrophoresis in the presence of cyclodextrin. The results also suggest that hyperluminescence occurs from multiphoton excitation of a single isolated serotonin molecule.

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Abbreviations

UV:

ultraviolet

RBL:

rat basophilic leukaemia

References

  1. A. S. Elhwuegi, Prog. Neuro-Psychopharmacol. Biol. Psychiatry, 2004, 28, 435–451.

    Article  CAS  Google Scholar 

  2. M. Asberg, Ann. N. Y. Acad. Sci., 1997, 836, 158–181.

    Article  CAS  Google Scholar 

  3. N. Beturquia, F. Couderc, V. Sauvinet, C. Ordset, S. Parrot, C. Bayle, B. Renaus and L. Denoroy, Electrophoresis, 2005, 26, 1071–1079.

    Article  Google Scholar 

  4. S. Okumoto, L. L. Looger, K. D. Michever, R. J. Reimer, S. J. Smith and W. B. Frommer, Proc. Natl. Acad. Sci. USA, 2005, 102, 8740–8745.

    Article  CAS  Google Scholar 

  5. J. Merz, Curr. Opin. Neurobiol., 2004, 14, 610–616.

    Article  Google Scholar 

  6. J. B. A. Ross, A. G. Szabo and C. W. V. Hogue, Methods Enzymol., 1997, 278, 151–190.

    Article  CAS  Google Scholar 

  7. S. Maiti, J. B. Shear, R. M. Williams, W. R. Zipfel and W. W. Webb, Science, 1997, 275, 530–532.

    Article  CAS  Google Scholar 

  8. R. M. Williams, J. B. Shear, W. R. Zipfel, S. Maiti and W. W. Webb, Biophys. J., 1999, 76, 1835–1846.

    Article  CAS  Google Scholar 

  9. J. B. Shear, C. Xu and W. W. Webb, Photochem. Photobiol., 1997, 65, 931–936.

    Article  CAS  Google Scholar 

  10. R. H. Bisby, M. Arvanitidis, S. W. Botchway, I. P. Clark, A. W. Parker and D. Tobin, Photochem. Photobiol. Sci., 2003, 2, 157–162.

    Article  CAS  Google Scholar 

  11. M. L. Gostkowski, J. Wei and J. B. Shear, Anal. Biochem., 1998, 260, 244–250.

    Article  CAS  Google Scholar 

  12. M. J. Gordon, E. Okerberg, M. L. Gostkowski and J. B. Shear, J. Am. Chem. Soc., 2001, 123, 10780–10781.

    Article  CAS  Google Scholar 

  13. J. Szejtli, Chem. Rev., 1998, 98, 1743–1753.

    Article  CAS  Google Scholar 

  14. P. Bortolus and S. Monti, Adv. Photochem., 1996, 21, 1–119.

    CAS  Google Scholar 

  15. S. Scypinski and L. J. C. Love, Anal. Chem., 1984, 56, 331–336.

    Article  CAS  Google Scholar 

  16. A. Orstan and J. B. A. Ross, J. Phys. Chem., 1987, 91, 2739–2745.

    Article  CAS  Google Scholar 

  17. R. E. Galian, A. V. Veglia and R. H. de Rossi, Analyst, 1998, 123, 1587–1591.

    Article  CAS  Google Scholar 

  18. C. Donze, E. Rizzarelli and G. Vecchio, Supramol. Chem., 1998, 10, 33–42.

    Article  CAS  Google Scholar 

  19. M. A. El-Kemary and I. M. El-Mehasseb, Talanta, 2004, 62, 317–322.

    Article  CAS  Google Scholar 

  20. K. S. S. P. Rao, S. M. Hubig, J. N. Moorthy and J. K. Kochi, J. Org. Chem., 1999, 64, 8098–8104.

    Article  CAS  Google Scholar 

  21. K. Lotte, R. Plessow and A. Brockhinke, Photochem. Photobiol. Sci., 2004, 3, 348–359.

    Article  CAS  Google Scholar 

  22. A. Chattopadhyay, R. Rukmini and S. Mukerjee, Biophys. J., 1996, 71, 1952–1960.

    Article  CAS  Google Scholar 

  23. S. W. Botchway, I. Barba, R. Jordan, R. Harmston, P. M. Haggie, S. P. Williams, A. M. Fulton, A. W. Parker and K. M. Brindle, Biochem. J., 2005, 390, 787–790.

    Article  CAS  Google Scholar 

  24. M. L. Gostkowski, R. Allen, M. L. Plenert, E. Okerberg, M. J. Gordon and J. B. Shear, Biophys. J., 2004, 86, 3223–3229.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Biosciences Research Institute, University of Salford, Salford, UK, M5 4WT

    Roger H. Bisby & Shakeela Dad

  2. Lasers for Science Facility, Rutherford Appleton Laboratory, Chilton, UK, OX11 0QX

    Stanley W. Botchway & Anthony W. Parker

Authors
  1. Roger H. Bisby
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  2. Stanley W. Botchway
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  3. Shakeela Dad
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  4. Anthony W. Parker
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Correspondence to Roger H. Bisby.

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Bisby, R.H., Botchway, S.W., Dad, S. et al. Single- and multi-photon excited fluorescence from serotonin complexed with ß-cyclodextrin. Photochem Photobiol Sci 5, 122–125 (2006). https://doi.org/10.1039/b508602g

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  • DOI: https://doi.org/10.1039/b508602g

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