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Reactive oxygen species and UV-B: effect on cyanobacteria

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Abstract

Reactive oxygen species (ROS) are involved in the damage and response of cyanobacteria to UV-B irradiation. In cyanobacteria, there are several targets for the potentially toxic ROS such as lipids, DNA and protein. The damage to photosynthetic apparatus induces the inhibition of photosynthesis that is mediated partially by ROS. UV-B-induced oxidative stress and oxidative damage increases with irradiation time and can be reversed after long-term irradiation. This raises the interesting question of whether cyanobacteria can acclimatize to the present UV-B stress. On one hand, ROS may also act as signal molecules and mediate the genetic regulation of photosynthetic genes and the induction of antioxidant enzymes. On the other hand, the efficient defense and repair system allows cyanobacteria to recover from the oxidative damage under moderate UV-B irradiation. In addition, the following methods are discussed: the fluorogenic probe 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), used to detect oxidative stress induced by UV-B; thiobarbituric acid reactive substances (TBARS), used to determine lipid peroxidation in cyanobacteria; fluorimetric analysis of DNA unwinding (FADU), used to quantify DNA strand breaks induced by ROS formation under UV-B stress.

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Abbreviations

APX:

ascorbate peroxidase

DCF:

2′,7′-dichlorofluorescein

DCFH:

2′,7′-dichlorodihydrofluorescein

DCFH-DA:

2′,7′-dichlorodihydrofluorescein diacetate

dsDNA:

double-strand DNA

FADU:

fluorimetric analysis of DNA unwinding

H2O2:

hydrogen peroxide

OH˙:

hydroxyl radical

MDA:

malondialdehyde (propanedial)

NAC:

N-acetylcysteine

PAR:

photosynthetically active radiation (400–700 nm)

PSI:

photosystem I

PSII:

photosystem II

ROS:

reactive oxygen species

1O2:

singlet oxygen

SOD:

superoxide dismutase

O2˙−:

superoxide radical

TBARS:

thiobarbituric acid reactive substances

UV-A:

ultraviolet A (315–400 nm)

UV-B:

ultraviolet B (280–315 nm).

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  1. Institut für Botanik und Pharmazeutische Biologie, Friedrich-Alexander-Universität, Staudtstr. 5, D-91058, Erlangen, Germany

    Yu-Ying He & Donat-P. Häder

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  1. Yu-Ying He
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  2. Donat-P. Häder
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Correspondence to Donat-P. Häder.

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He, YY., Häder, DP. Reactive oxygen species and UV-B: effect on cyanobacteria. Photochem Photobiol Sci 1, 729–736 (2002). https://doi.org/10.1039/b110365m

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