Abstract
The Vel blood group antigen is expressed on the red blood cells of most individuals. Recently, we described that homozygosity for inactivating mutations in SMIM1 defines the rare Vel-negative phenotype. Still, Vel-positive individuals show great variability in Vel antigen expression, creating a risk for Vel blood typing errors and transfusion reactions. We fine-mapped the regulatory region located in SMIM1 intron 2 in Swedish blood donors, and observed a strong correlation between expression and rs1175550 as well as with a previously unreported tri-nucleotide insertion (rs143702418; C > CGCA). While the two variants are tightly linked in Caucasians, we separated their effects in African Americans, and found that rs1175550G and to a lesser extent rs143702418C independently increase SMIM1 and Vel antigen expression. Gel shift and luciferase assays indicate that both variants are transcriptionally active, and we identified binding of the transcription factor TAL1 as a potential mediator of the increased expression associated with rs1175550G. Our results provide insight into the regulatory logic of Vel antigen expression, and extend the set of markers for genetic Vel blood group typing.
Similar content being viewed by others
Introduction
Recently, we and two other research groups identified a previously unreported erythroid gene, Small Integral Membrane Protein 1 (SMIM1), as the locus of the Vel blood group system1,2,3. We showed that individuals who lack the Vel blood group antigen (about 1/1,200 in Sweden and 1/4,000 globally) are homozygous for an inactivating 17-bp deletion in SMIM1 exon 3.
While these findings provide a molecular background for the Vel-negative (Vel−) phenotype, they do not explain the recurrent clinical observation that Vel-positive (Vel+) individuals show great variability in antigen expression. Weakly Vel+ erythrocytes (Vel+weak) can be mistyped as Vel− by routine serological phenotyping, creating risk for transfusion reactions in patients with anti-Vel inadvertently transfused with Vel+weak erythrocytes. While some of the variation can be attributed to heterozygosity for the 17-bp deletion1, this mutation does not account for all the variation. The common SNP rs1175550 (A > G, minor allele frequency, MAF = 0.22 in Caucasians), located in intron 2 of SMIM1, associates with mean corpuscular hemoglobin concentration (MCHC)4, and individuals who carry the minor allele (rs1175550G) express SMIM1 at a higher level than individuals who carry the major allele (rs1175550A)2,5,6. However, it is not known whether rs1175550 itself is causal, or a proxy for another (as yet undetected) causal variant. Moreover, the molecular effects of this variant have not been defined, or whether additional variants modulate SMIM1 expression.
To address these questions, we fine-mapped a candidate regulatory region in SMIM1 intron 2 containing rs1175550 and multiple erythroid transcription factor binding sites in Swedish and African American blood donors. We identified rs1175550 and a previously unreported tri-nucleotide insertion (rs143702418; C > CGCA) as correlated with SMIM1 and Vel antigen expression. While these variants, and their effects on expression, were inseparable in the Swedish samples, their effects could be separated in the African American samples where the linkage disequilibrium in SMIM1 intron 2 was not as tight. Our data show that rs1175550 and rs143702418 independently modulate SMIM1 expression, although the effect of rs1175550 dominated that of rs143702418. Using gel shift assays, we found that both variants influence nuclear protein binding, and that the strong effect of rs1175550 may be mediated by differential binding of TAL1.
Results
rs1175550 is located in a regulatory region in SMIM1 intron 2
To understand the role of rs1175550, we examined its neighborhood in SMIM1 intron 2 for transcription factor binding sites using ChIP-seq data in the Encyclopedia of DNA Elements compendium (ENCODE)7. This revealed a 500 bp region with increased acetylation of lysine 27 on histone 3, increased DNaseI hypersensitivity, and binding sites for multiple transcription factors, including the erythroid factors GATA-1, TAL1 and ZBTB7A. The region contained eight sequence variants annotated with a MAF of more than 1% in dbSNP 1448 (Fig. 1a).
rs1175550 and rs143702418 associate with SMIM1 and Vel expression
To test for correlations with SMIM1 and Vel expression, we sequenced the identified region in 150 Vel+ Swedish blood donors. In the same individuals, we quantified SMIM1 and Vel antigen expression by quantitative PCR and flow cytometry. We detected all eight candidate sequence variants at frequencies similar to those reported in the 1000 Genomes catalog, phase 39 containing fully sequenced genomes from 2,504 individuals grouped into five superpopulations (African, American, East Asian, European and South Asian) (Table 1). The rs1175550 variant was strongly associated with SMIM1 mRNA levels (p = 2.1∙10−8), Vel antigen expression (p = 4.0∙10−15) and SMIM1 protein on erythrocyte membranes, with the G allele correlated with higher expression (Fig. 1b–d). Interestingly, we found almost identical allele frequencies and correlation values for a previously unreported C > CGCA insertion rs143702418 (rs70940313 in reverse complement), located only 96 basepairs upstream of rs1175550 (Table 1, Supplementary Figs S1 and S2). We found no significant correlations for the remaining six variants (Supplementary Figs S1 and S2). The two variants showed near-perfect linkage disequilibrium (LD), with rs1175550G being linked to rs143702418CGCA in 149 out of 150 samples (Table 1 and Supplementary Data S1). Furthermore, in the three predominant SMIM1 intron 2 alleles (comprising 96% of all identified alleles) in Europeans in the 1000 Genomes project the rs1175550G/rs143702418CGCA and rs1175550A/rs143702418C genotypes were always linked (Supplementary Fig. S3). Thus, both rs1175550 and rs143702418 associate with SMIM1 and Vel antigen expression, yet the strong LD precluded statistical separation of their effects using samples from individuals of European ancestry.
rs1175550 and rs143702418 independently influence SMIM1 and Vel antigen expression
Hypothesising that the LD between rs1175550 and rs143702418 might be different in other populations, we examined the repertoire of SMIM1 intron 2 alleles in the 1000 Genomes catalog. We observed 32 different alleles, although nine of these were only present in a single individual in 1000 Genomes, and could therefore represent sequencing artifacts. Analysis of alleles detected in at least two individuals showed that these cluster mainly by rs1175550 genotype, indicating that this variant appeared evolutionarily before the other variants in the region (Supplementary Fig. S4).
Analysing the 1000 Genomes data, we noted a difference in genotype frequency between the two variants in the African super population (allele frequency 0.60 and 0.26 for rs1175550G and rs143702418CGCA, respectively), meaning the two variants are not in tight LD: the frequency of the unlinked rs1175550G/rs143702418C allele was 0.26 in African Americans, compared to 0.01 in Europeans (Fig. 2a). To deconvolve the effects of rs1175550 and rs143702418, and thereby assess their causality, we analysed samples from 202 African American blood donors. These samples showed a broader repertoire of SMIM1 intron 2 alleles when compared to the Swedish samples, including alleles where the linkage between rs1175550 and rs143702418 was broken (Supplementary Data S2).
Genotype frequencies in the African American sample set matched those in the 1000 Genomes catalog (Table 1). In this sample set, we found rs1175550 to be associated with SMIM1 mRNA and Vel antigen expression (p = 1.0∙10−15 for Vel antigen expression; p = 7.0∙10−8 for SMIM1 mRNA), whereas rs143702418 showed weaker association (p = 0.0002112 for SMIM1 mRNA; p = 0.0103 for Vel antigen expression) (Fig. 2b–e). None of the other candidate variants showed any significant correlation (Supplementary Figs S5 and S6).
To test if the association with rs143702418 represents an independent effect, we used multiple linear regression including both rs1175550 and rs143702418 in the model. We found that samples heterozygous for rs143702418 had significantly lower Vel antigen expression (effect estimate, p = 0.00398) compared to homozygosity for rs143702418C (Fig. 3a), and observed a trend towards further decrease for samples homozygous for rs143702418CGCA (effect estimate, p = 0.08939). This was consistent with an increase in the model R2 as compared to a model including rs1175550 alone (adjusted R2 = 0.5237 vs. 0.5064 for rs1175550 alone; p < 2.2∙10−16 for model), indicating that rs143702418 has a small but independent effect (Fig. 3a, Supplementary Table S1). This trend was mirrored by the mRNA expression levels, although it was not statistically significant, possibly due to the limited sample size (Fig. 3b). No independent effects were observed for the other six candidate SNPs (Supplementary Table S1).
rs1175550 and rs143702418 are transcriptionally active
Sequence analysis showed that rs1175550 and rs143702418 are located at predicted binding sites for erythroid transcription factors. For rs1175550, the major allele predicts a non-canonical GATA-1 site, GATT, modified by the minor allele to GGTT. For rs143702418, we observed that the major allele comprises a core motif for KLF1 (CACCC), which is modified by the minor allele to CACGCACC.
For functional validation of rs1175550 and rs143702418, we made reporter constructs corresponding to the four theoretical alleles containing the combinations of these two biallelic variants (Fig. 4a). Consistent with our association data, luciferase assays in K562 and HEL erythroleukemia cells showed higher activity with both rs1175550G and rs143702418C (Fig. 4b), and both constructs with rs143702418C showed higher activity than those with rs143702418CGCA (K562 p = 0.000683; HEL p = 0.002703). The highest luciferase activity was found for the construct also carrying the rs1175550G (p = 0.03448 in the HEL cells). Gel shift assays with nuclear extracts from K562 cells showed distinct binding patterns with probes mapping to the minor and major alleles for both rs143702418 and rs1175550 (Fig. 5a,b), most notably a distinct, shifted band only seen with the rs1175550G probe (Fig. 5b, lane 4). These results further support that the two variants independently modulate SMIM1 and Vel antigen expression, although rs1175550 has a more powerful effect.
Identification of TAL1 as a candidate factor underlying the increased SMIM1 expression associated with rs1175550G
Based on ChIP-seq data from ENCODE (Fig. 1a), we identified GATA-1, KLF1, TAL1 and ZBTB7A as candidate factors for regulating SMIM1 expression. These factors have been previously identified as erythroid, and their temporal expression profiles in erythrocyte development follow that of SMIM1 (Supplementary Fig. S7)10. We also included Gfi-1B as its binding motif AATC (reverse-complement GATT) matched the sequence at rs1175550, and it has been shown to associate with GATA-1 and mediate transcriptional repression11,12,13.
We carried out gel shift analyses with antibodies to the selected transcription factors. Firstly, we observed a supershift with anti-GATA-1 for rs1175550 (Fig. 5b, lanes 3,6). However, this signal was not allele-specific and additional analyses with probes mutated at the predicted non-canonical GATA-1 site at rs1175550 yielded similar results, indicating that GATA-1 does not bind directly to rs1175550 (Fig. 5b, lanes 8,10). Secondly, with anti-Gfi-1B, we observed that the strong band seen with both rs1175550A and rs1175550G probes was weakened with anti-Gfi-1B, whereas the allele-specific band observed only with the rs117550G probe remained unaffected (Fig. 5a, lanes 3,9). These data indicate that GATA-1 and Gfi-1B bind near rs1175550 (directly or indirectly) regardless of genotype, making it unlikely that the increased expression associated with rs1175550G is explained by altered binding of GATA-1 or Gfi-1B.
In contrast to the non-allele-specific reactions seen with anti-GATA-1 and anti-Gfi-1B, we achieved suppression of the rs1175550G-specific signal with anti-TAL1 (Fig. 6a, lane 10). No similar effects were seen with anti-Gfi-1B, anti-ZBTB7A or anti-KLF1 (Fig. 6a), while the TAL1 effect was dose-dependent (Fig. 6b,c). This is particularly interesting since only three bps upstream of the rs1175550 is a near-perfect match for the E-box motif CAGNTG, which is a known binding site for TAL1 in heterodimer complexes with E12 and E4714. Finally, we observed no allele-specific supershift or suppression for rs143702418 using these antibodies (Fig. 5a). In summary, our results suggest that differential binding of TAL1, or a TAL1-containing complex, could mediate the increase in SMIM1 and Vel antigen expression associated with rs1175550G.
Discussion
The variation in antigen expression among Vel+ individuals is clinically important, as it may lead to erroneous typing of Vel+ blood as Vel− with anti-Vel sera15,16. While rs1175550 zygosity may explain some of this variation, the causality of this sequence variant has been unclear. Fine-mapping the genomic neighbourhood of rs1175550, a regulatory region in SMIM1 intron 2, we discovered that the previously unreported trinucleotide insertion rs143702418 also correlates with SMIM1 and Vel antigen expression. While the effects of rs1175550 and rs143702418 were inseparable in Swedish samples, deconvolution in African American samples revealed that rs1175550 and rs143702418 both modulate Vel expression, although rs1175550 has the strongest effect. Luciferase and gel shift assays supported that both variants are transcriptionally active.
Although the exact mechanisms remain to be elucidated, we identified increased binding of TAL1 as a potential explanation for the increased SMIM1 expression associated with rs1175550G. This is in concordance with recently published data suggesting that TAL1 preferentially binds to rs1175550G17. Since the discussed TAL1 and GATA-1/Gfi-1B binding motifs are only three base pairs apart, one could speculate that steric hindrance does not allow these factors to bind simultaneously and that the A > G substitution favours TAL1 binding.
In conclusion, our findings provide novel insight into the regulation of SMIM1 and the Vel blood group antigen, and provide further reason to take rs1175550 and rs143702148 into account when evaluating the correlation between Vel blood group phenotype and genotype. Insight into what governs blood group expression levels on erythroid cells can be important for our understanding of host-pathogen interactions since many blood group molecules serve as involuntary receptors for microbial agents and may therefore act as susceptibility markers for disease. Even if no such role has yet been proven for SMIM1, it has been hypothesised to be a long-sought malaria receptor1.
Recently, trans-ancestry association analysis has been proposed as a way to refine associations between genetic variants, quantitative traits and human diseases, yet only a few examples have been published so far18,19,20. Here we exploited pre-existing population-based data sets and trans-ancestry association analysis to deconvolve highly correlated effects. We predict that these approaches will be increasingly important for identifying the molecular-genetic effects of GWAS loci.
Methods
Blood samples
We used anonymised, peripheral blood samples from 150 Swedish blood donors (routine donations at Clinical Immunology and Transfusion Medicine, Lund, Sweden), and 202 self-declared African-American blood donors (routine donations at New York Blood Center, New York, NY, USA). No donors were approached solely for the purpose of this study. Genomic DNA and total RNA were isolated using standard methods. Samples were screened for the 17-bp deletion in SMIM1 exon 31 and carriers were excluded.
Sanger sequencing
For Sanger sequencing of the 500-bp candidate regulatory region (hg38 coordinates chr1:3,774,672–3,775,171), we used the following primers: forward primer 1: 5′-TCTGTAGGGCCTCCCCAA-3′, forward primer 2: 5′-ACAGGGACCGGTCTAGCTGTA-3′, reverse primer 1: 5′-CTCCTAACAGCAGCCTCAGAG-3′ and reverse primer 2: 5′-TGTTAAGTCAGGCGACAGACC-3′. PCR products were sequenced using the BigDye Terminator v3.1 Cycle Sequencing kit on an ABI 3500 Dx Genetic Analyzer (Applied Biosystems). Additional Sanger sequencing was done at GATC Biotech, Germany. For peak calling and base determination we used CodonCode Aligner v4.1 software (CodonCode Corporation, USA).
Reverse transcription quantitative PCR
Total RNA was extracted by Trizol LS purification (Ambion) and converted to cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative PCR was carried out on cDNA using an assay mix specific for SMIM1 (Applied Biosystems assay no. Hs01369635_g1). SMIM1 expression was quantified as 2−ΔΔCT against the sample having the highest expression value. For normalisation across multiple qPCR runs these samples were included in each experiment.
Flow cytometry
Red blood cells (RBCs) were incubated with human polyclonal anti-Vel followed by PE-conjugated F (ab′)2 Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, #109–116–098) as described previously1. Cells were analysed on a FACS Calibur flow cytometer using CellQuest v3.3 and FACS Diva v6.1.3 software (Becton Dickinson). Values were taken as median fluorescence intensity (MFI) and all samples were normalised against the sample with the highest value in either collection.
Western blot analysis
RBC membranes were prepared as described previously1, and analysed using Any kD Mini-PROTEAN TGX Stain-Free gels and the V3 Western Workflow (BioRad Laboratories). Following transfer, PVDF membranes were incubated with rabbit polyclonal anti-SMIM1 followed by horseradish peroxidase (HRP)-labelled goat anti-rabbit IgG (Agrisera, Sweden). Bands were visualised on ChemiDoc Touch (BioRad Laboratories) and analysed using ImageLab software v5.2 (BioRad Laboratories).
Luciferase assay
Four DNA fragments of 117 bp or 120 bp (synthesised by Integrated DNA Technologies, USA) corresponding to the possible combinations created by the two variants rs143702418C/CGCA and rs1175550A/G (Supplementary Table S2) were inserted upstream of the luciferase gene in the pGL3-Basic luciferase vector (Promega) using restriction enzymes NheI and HindIII. DH5α competent E. coli cells were transformed with each construct, correct insertion was confirmed by sequencing and positive clones were amplified and purified with Plasmid Midi Kit (Qiagen).
K562 and HEL cells were cultured at 37 °C and 5% CO2 in RPMI 1640 medium (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (Gibco). 2∙106 cells were mixed with 2 μg construct and 0.2 μg Renilla luciferase construct and electroporated at 960 μFD and 280 V. Following incubation for 24 hours, dual luciferase assays were performed according to manufacturer’s protocol (Dual-Luciferase Reporter Assay System, Promega). All experiments were performed in triplicate.
Electrophoretic mobility shift assay
Nuclear proteins were extracted from human K562 erythroleukemia cells as described21 and gel shifts were performed as described previously22. We used the following double-stranded probes (variant underlined): for rs143702418: 5′-GCCCTGCCCCACCCCCCCCTCCC-3′ and 5′-GCCCTGCCCCACGCACCCCCCCTCCC-3′, and for rs1175550: 5′-CTGCAGCCTAGATTGGGCCACAA-3′ and 5′-CTGCAGCCTAGGTTGGGCCACAA-3′. Additionally, we used two “scrambled” probes with the region surrounding the rs1175550 variant mutated (underlined): 5′-CTGCAGCCTCTTCGGGGCCACAA-3′ and 5′-CTGCAGCCTCCGACGGGCCACAA-3′. All probes were biotin-labeled at the 5′ end of both strands, unlabeled competitor probes with identical sequences were used to test for specificity. Supershift assays were performed using anti-GATA-1 (ActiveMotif, #39319), anti-Gfi-1B (B-7, Santa Cruz Biotechnology), anti-KLF1 (H-210, Santa Cruz Biotechnology), anti-LRF (ZBTB7A, H-6, Santa Cruz Biotechnology) and anti-TAL1 (ActiveMotif, BTL-73) antibodies. 2 μg antibody was added to the reaction mix and incubated 1 hour on ice, before addition of labeled probes, which in turn incubated 30 minutes at room temperature.
Statistical analysis
Kruskal-Wallis one-way analysis of variance with Dunn’s multiple comparison tests as well as multiple linear regression models were used to test for association between genotypes vs. SMIM1 (RT-qPCR) or Vel antigen expression (flow cytometry). In a first analysis, we included only rs1175550 as the explanatory variable. To assess the effect of variants conditioned on rs1175550, we carried out additional analyses using bivariate models with each variant together with rs1175550. For the phylogenetic analysis, we used phased genotyping data from The 1000 Genomes Project, phase 3 for the studied region in SMIM1. To reconstruct the phylogeny, we calculated the Euclidean distance between the different alleles, and clustered the alleles using the neighbour-joining method23. R version 3.2.2, Graphpad Prism version 7.0a and Microsoft Excel were used for data analyses.
Additional Information
How to cite this article: Christophersen, M. K. et al. SMIM1 variants rs1175550 and rs143702418 independently modulate Vel blood group antigen expression. Sci. Rep. 7, 40451; doi: 10.1038/srep40451 (2017).
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
References
Storry, J. R. et al. Homozygosity for a null allele of SMIM1 defines the Vel-negative blood group phenotype. Nat Genet 45, 537–541, doi: 10.1038/ng.2600 (2013).
Cvejic, A. et al. SMIM1 underlies the Vel blood group and influences red blood cell traits. Nat Genet 45, 542–545, doi: 10.1038/ng.2603 (2013).
Ballif, B. A. et al. Disruption of SMIM1 causes the Vel- blood type. EMBO Mol Med 5, 751–761, doi: 10.1002/emmm.201302466 (2013).
van der Harst, P. et al. Seventy-five genetic loci influencing the human red blood cell. Nature 492, 369–375, doi: 10.1038/nature11677 (2012).
Haer-Wigman, L. et al. Impact of genetic variation in the SMIM1 gene on Vel expression levels. Transfusion 55, 1457–1466, doi: 10.1111/trf.13014 (2015).
Christophersen, M. K. et al. Genetic variants regulate expression of the Vel blood group antigen. Vox Sanguinis 107 Suppl 1, 16, doi: 10.1111/vox.12153 (2014).
ENCODE Project Consortium. An integrated encyclopedia of DNA elements in the human genome. Nature 489, 57–74, doi: 10.1038/nature11247 (2012).
Sherry, S. T. et al. dbSNP: the NCBI database of genetic variation. Nucleic Acids Res 29, 308–311 (2001).
The 1000 Genomes Project Consortium. A global reference for human genetic variation. Nature 526, 68–74, doi: 10.1038/nature15393 (2015).
Keller, M. A. et al. Transcriptional regulatory network analysis of developing human erythroid progenitors reveals patterns of coregulation and potential transcriptional regulators. Physiol Genomics 28, 114–128, doi: 10.1152/physiolgenomics.00055.2006 (2006).
Huang, D. Y., Kuo, Y. Y. & Chang, Z. F. GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells. Nucleic Acids Res 33, 5331–5342, doi: 10.1093/nar/gki838 (2005).
Rodriguez, P. et al. GATA-1 forms distinct activating and repressive complexes in erythroid cells. EMBO J 24, 2354–2366, doi: 10.1038/sj.emboj.7600702 (2005).
Kuo, Y. Y. & Chang, Z. F. GATA-1 and Gfi-1B interplay to regulate Bcl-xL transcription. Mol Cell Biol 27, 4261–4272, doi: 10.1128/MCB.02212-06 (2007).
Hsu, H. L., Cheng, J. T., Chen, Q. & Baer, R. Enhancer-binding activity of the tal-1 oncoprotein in association with the E47/E12 helix-loop-helix proteins. Mol Cell Biol 11, 3037–3042 (1991).
Issitt, P. D. et al. Anti-Vel 2, a new antibody showing heterogeneity of Vel system antibodies. Vox Sang 15, 125–132 (1968).
Sussman, L. N. & Miller, E. B. [New blood factor: Vel.]. Rev Hematol 7, 368–371 (1952).
Ulirsch, J. C. et al. Systematic Functional Dissection of Common Genetic Variation Affecting Red Blood Cell Traits. Cell 165, 1530–1545, doi: 10.1016/j.cell.2016.04.048 (2016).
Iyengar, S. K. et al. Genome-Wide Association and Trans-ethnic Meta-Analysis for Advanced Diabetic Kidney Disease: Family Investigation of Nephropathy and Diabetes (FIND). PLoS Genet 11, e1005352, doi: 10.1371/journal.pgen.1005352 (2015).
Morris, A. P. Transethnic meta-analysis of genomewide association studies. Genet Epidemiol 35, 809–822, doi: 10.1002/gepi.20630 (2011).
Kato, N. et al. Trans-ancestry genome-wide association study identifies 12 genetic loci influencing blood pressure and implicates a role for DNA methylation. Nat Genet 47, 1282–1293, doi: 10.1038/ng.3405 (2015).
Andrews, N. C. & Faller, D. V. A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. Nucleic Acids Res 19, 2499 (1991).
Ajore, R., Dhanda, R. S., Gullberg, U. & Olsson, I. The leukemia associated ETO nuclear repressor gene is regulated by the GATA-1 transcription factor in erythroid/megakaryocytic cells. BMC Mol Biol 11, 38, doi: 10.1186/1471-2199-11-38 (2010).
Saitou, N. & Nei, M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425 (1987).
Acknowledgements
We thank Britt Thuresson and Karina Vidovic for their assistance. The work was supported by research grants from the Swedish Foundation for Strategic Research (KF10–0009 and ICA08–0057), the Marianne and Marcus Wallenberg Foundation (2010.0112), the Knut and Alice Wallenberg Foundation (2012.0193 and 2014.0312), the Swedish Medical Research Council (2014–71X-14251), the Crafoord foundation, the Royal Swedish Academy of Science, governmental ALF grants from Region Skåne, the Medical Faculty at Lund University, and the Swedish Society of Medicine.
Author information
Authors and Affiliations
Contributions
M.K.C., M.J., J.R.S., M.L.O. and B.N. designed the research; M.K.C., M.J., R.A., J.R.S., K.W.L. and B.N. performed experiments and/or data analysis; C.M.W. and S.V. supplied African American samples. M.K.C. drafted the manuscript. All authors contributed to data interpretation and the final manuscript.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
About this article
Cite this article
Christophersen, M., Jöud, M., Ajore, R. et al. SMIM1 variants rs1175550 and rs143702418 independently modulate Vel blood group antigen expression. Sci Rep 7, 40451 (2017). https://doi.org/10.1038/srep40451
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/srep40451
- Springer Nature Limited
This article is cited by
-
Elucidation of the low-expressing erythroid CR1 phenotype by bioinformatic mining of the GATA1-driven blood-group regulome
Nature Communications (2023)
-
Functional dissection of inherited non-coding variation influencing multiple myeloma risk
Nature Communications (2022)
-
Cataloguing experimentally confirmed 80.7 kb-long ACKR1 haplotypes from the 1000 Genomes Project database
BMC Bioinformatics (2021)