Skip to main content
SpringerLink
Log in

Induction of endogenous β-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice

  • Short Report
  • Published:
Oncogene Submit manuscript

Abstract

Studies of the response of p53-lacZ transgenic mice have uncovered an unexpected induction of endogenous acid-β-galactosidase activity following whole body irradiation. Strong induction of endogenous enzyme activity is seen in a variety of mouse strains commonly used in the production of transgenes. The induction of endogenous enzyme activity therefore complicates the analysis of p53-lacZ transgenes and may also influence the analysis of radiation responses in other lacZ-reporter mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Gottlieb E, Haffner R, King A, Asher G, Gruss P, Lonai P, Oren M . 1997 EMBO J. 16: 1381–1390

  • Hatton JD, Lin L . 1992 J. Neurosci. Meth. 45: 147–153

  • Hendrikx PJ, Martens ACM, Visser JWM, Hagenbeek A . 1994 Anal. Biochem. 222: 456–460

  • Komarova EA, Chernov MV, Franks R, Wang K, Armin G, Zelnick CR, Chin DM, Bacus SS, Stark GR, Gudkov AV . 1997 EMBO J. 16: 1391–1400

  • Lorimore SA, Coates PJ, Scobie GE, Milne G, Wright EG . 2001 Inflammatory-type responses after exposure to ionizing radiation in vivo: a mechanism for radiation-induced bystander effects? Oncogene 20: 7085–7095

    Article  CAS  Google Scholar 

  • MacCallum DE, Hupp TR, Midgley CA, Stuart D, Campbell SJ, Harper A, Walsh FS, Wright EG, Balmain A, Lane DP, Hall PA . 1996 Oncogene 13: 2576–2587

  • Nowroozi N, Kim S, Gupta A, Warita H, Zernik J . 1998 J. Craniofac. Genet. Dev. Biol. 18: 51–57

  • Weiss DJ, Liggit D, Clark JG . 1999 Histochem. J. 31: 231–236

Download references

Author information

Authors and Affiliations

  1. Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, Dundee, DD1 9SY, UK

    Philip J Coates, Sally A Lorimore, Brigitte A Rigat & Eric G Wright

  2. Department of Surgery and Molecular Oncology, University of Dundee, Ninewells Hospital and Medical School, Dundee, DD1 9SY, UK

    David P Lane

Authors
  1. Philip J Coates
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sally A Lorimore
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Brigitte A Rigat
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. David P Lane
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eric G Wright
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eric G Wright.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Coates, P., Lorimore, S., Rigat, B. et al. Induction of endogenous β-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice. Oncogene 20, 7096–7097 (2001). https://doi.org/10.1038/sj.onc.1204904

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1204904

  • Springer Nature Limited

Keywords

This article is cited by

Search

Navigation