A bioluminescent reporter strain, Acinetobacter sp. DF4-8, was constructed for the detection of phenol by inserting a mopR-like promoter upstream of the Vibrio fischeri bioluminescent luxCDABE gene cassette in a modified mini-Tn5 construct. When introduced into the chromosome of Acinetobacter sp. DF4, the bioreporter produced a sensitive bioluminescent response to phenol at concentrations ranging from 2.5 to 100 ppm. This response was linear (R 2=0.986) in the range from 20 to 90 ppm. A significant bioluminescent response was also recorded when strain DF4-8 was incubated with slurries from aged, phenol-contaminated soil.
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Received 13 February 2002/ Accepted in revised form 11 July 2002
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Abd-El-Haleem, D., Ripp, S., Scott, C. et al. A luxCDABE-based bioluminescent bioreporter for the detection of phenol. J Ind Microbiol Biotech 29, 233–237 (2002). https://doi.org/10.1038/sj.jim.7000309
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DOI: https://doi.org/10.1038/sj.jim.7000309