Most DNA methods for species identification of animal tissues test the presence/absence of one species per assay, requiring several tests for a complete analysis and prior knowledge of the species that are potentially present in the sample. Here we demonstrate that PCR with fluorescently labeled MIR (mammalian-wide interspersed repeat) primers generate fingerprints that are suitable for rapid identification of known and unknown species on an automatic sequencing apparatus and with computer-assisted data processing. The method allows the analysis of processed meat samples and offers a convenient alternative to sequencing of mitochondrial DNA.
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Received 19 December 1997/ Accepted in revised form 15 June 1998
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Buntjer, J., Lenstra, J. Mammalian species identification by interspersed repeat PCR fingerprinting. J Ind Microbiol Biotech 21, 121–127 (1998). https://doi.org/10.1038/sj.jim.2900540
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DOI: https://doi.org/10.1038/sj.jim.2900540