Abstract
Soil salinity affects the plant growth and productivity detrimentally, but Solanum chilense, a wild relative of cultivated tomato (Solanum lycopersicum L.), is known to have exceptional salt tolerance. It has precise adaptations against direct exposure to salt stress conditions. Hence, a better understanding of the mechanism to salinity stress tolerance by S. chilense can be accomplished by comprehensive gene expression studies. In this study 1-month-old seedlings of S. chilense and S. lycopersicum were subjected to salinity stress through application of sodium chloride (NaCl) solution. Through RNA-sequencing here we have studied the differences in the gene expression patterns. A total of 386 million clean reads were obtained through RNAseq analysis using the Illumina HiSeq 2000 platform. Clean reads were further assembled de novo into a transcriptome dataset comprising of 514,747 unigenes with N50 length of 578 bp and were further aligned to the public databases. Genebank non-redundant (Nr), Viridiplantae, Gene Ontology (GO), KOG, and KEGG databases classification suggested enrichment of these unigenes in 30 GO categories, 26 KOG, and 127 pathways, respectively. Out of 265,158 genes that were differentially expressed in response to salt treatment, 134,566 and 130,592 genes were significantly up and down-regulated, respectively. Upon placing all the differentially expressed genes (DEG) in known signaling pathways, it was evident that most of the DEGs involved in cytokinin, ethylene, auxin, abscisic acid, gibberellin, and Ca2+ mediated signaling pathways were up-regulated. Furthermore, GO enrichment analysis was performed using REVIGO and up-regulation of multiple genes involved in various biological processes in chilense under salinity were identified. Through pathway analysis of DEGs, “Wnt signaling pathway” was identified as a novel pathway for the response to the salinity stress. Moreover, key genes for salinity tolerance, such as genes encoding proline and arginine metabolism, ROS scavenging system, transporters, osmotic regulation, defense and stress response, homeostasis and transcription factors were not only salt-induced but also showed higher expression in S. chilense as compared to S. lycopersicum. Thus indicating that these genes may have an important role in salinity tolerance in S. chilense. Overall, the results of this study improve our understanding on possible molecular mechanisms underlying salt tolerance in plants in general and tomato in particular.
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Introduction
Soil salinity is one of the major abiotic stresses that significantly affects the plant growth and crop yields. Salinity stress can have a major impact on the global food production1,2. To date, approximately 10% of the world’s total land area (950 Mha), 20% of the world’s aerable land (300 Mha), and 50% of the total irrigated land (230 Mha) are affected by soil salinization. Further, it is expected to influence 50% of total cultivated land in 2050 at a disquieting rate3,4. Every year almost 12 billion US$ are globally lost due to salt stress that significantly affects the agricultural production5,6. High soil salinity stress affects plants, mainly in three ways: (a) ionic stress arising from increased levels of toxic ions such as Na+ and Cl− causing serious ionic imbalance, (b) osmotic stress due to the limited water availability leading to augmented osmotic pressure, and (c) oxidative damage, ultimately leading to the plant growth retardation and death7,8,9. Plants have to generate a series of significant salt-tolerance mechanisms to minimize the adverse effects of salt stress, and develop various physiological, biochemical, and molecular processes. This is acheived by regulating the expression of salt-responsive genes and other sophisticated strategies for proper membrane transport, ion transport, signal transduction, redox regulation, metabolic networks, transcription factors, amine and polyamine metabolism, oxidation–reduction, phyto-hormones, and reactive oxygen species (ROS) scavenging10,11,12.
Mechanisms for improved salt tolerance are well-recognized in Arabidopsis and other crops, which mainly comprise of calcium-dependent protein kinase (CDPK) pathway, salt overly sensitive (SOS) pathway, and mitogen-activated protein kinase (MAPK) pathway13,14. Plasma membrane Na+/H+ antiporter such as SOS1 is involved in sodium exclusion from the cytosol15. SOS2 [Calcineurin B-like protein (CBL)-CBL-interacting protein kinase (CIPK)] is a serine/threonine-protein kinase which interacts with SOS3-calcium sensor (CBL) to regulate the activity of Na+/H+ exchanger SOS1 by which these complexes confer salt resistance15,16,17. Interaction of CBL1 with CIPK24, CIPK25, and CIPK26 is known to regulate the Na+/K+ homeostasis18. Plasma membrane Na+/H+ antiporters (SOS1) that are generated in response to salt stress were also identified in Populus euphratica19. Additionally, several specific genes/gene families such as high-affinity K+ transporter (HKT) and Na+/H+ antiporters (NHX) conferring salinity tolerance have been reported in Arabidopsis thaliana20. Moreover, the contribution of cytochrome P450 genes in salt stress response has been established in the salt-tolerant tree species Populus euphratica21. Besides these, several transcription factors (TFs) belonging to bZIP, MYB, bHLH, C2H2-Dof, AP2-EREBP, and WRKY super families have been reported to play crucial role in imparting salt tolerance response in plants22,23,24.
Ca2+ plays a key role in plant adaptation against critical stress conditions and works as a second messenger in regulating growth and development of plants25. Ca2+ sensors are classified mainly into three families, comprising calcineurin B-like proteins, Ca2+-dependent protein kinases (CDPKs), and calmodulin26,27,28. Many transcription factors are directly activated by Ca2+/calmodulin, containing MYBs29, calmodulin binding transcription activators (CAMTAs)30 and GT-element-binding-like proteins31. Sulphur acclimatization also plays an essential role in the salt tolerance mechanism in plants through adaptation of metabolic modifications32,33. S-adenosyl methionine and arginine decarboxylase (ADC) are involved in polyamines (PAs) synthesis such as putrescine (Put) which is known to improve tolerance against salinity stress34,35.
Tomato (Solanum lycopersicum L.) is the second most consumed vegetable crop after potato36 and certainly the globally-widespread garden crop37. Tomato fruits are rich source of natural pigments such as lycopene, β-carotene, dietary fiber, vitamin A, vitamin E, potassium, and vitamin C. Lycopene, a pigment enriched with antioxidant properties is used for the therapeutic purpose to prevent cancer38. Most of the cultivated tomatoes are known to be highly sensitive to salinity at different stages of plant growth and fail to produce higher yield37. Therefore, it becomes necessary to identify the key salt responsive genes that confer salt tolerance and facilitate plant survival during high salinity39. Estimation by Miller and Tanksley40 suggested that cultivated tomato families within S. lycopersicum have less than five per cent of the total genetic variation. Therefore, genes involved in salt tolerance may not occur in the cultivated tomato species37. However, wild relatives of tomato such as S. chilense, S. pimpinellifolium, S. peruvianum, S. hirsutum, and S. pennellii are known to possess genes essential for improvement of abiotic stress tolerance.
However, till date, there is no sufficient information available about the salinity tolerance in S. chilense. Hence, it becomes more important to know stress-responsive mechanisms evolved by S. chilense that allows it to survive in life-threatening aridity, high temperature, drought, and salt stress environment in Northern Chile of Atacama Desert41. Earlier studies have suggested that S. chilense shows a promising response to drought and salt-tolerance as compared to the cultivated tomato42,43,44. Besides these, enhanced salt adaptation and tolerance activities such as mobilization of H2O2 components, higher plant water holding capacity, growth, and increased enzymatic antioxidant capacity are well-documented in S. chilense42,44,45,46. Unfortunately, the information on molecular salt stress mechanisms of this species during salinity is lacking. Therefore, it is imperative to elucidate gene expression profiles of this species under salt stress.
Capturing expression pattern of salt-responsive genes by high-throughput sequencing technologies makes it possible to resolve important questions realated to salinity stress tolerance47,48. RNASeq technology remains an essential tool for elucidating responsive mechanisms against salinity conditions in tomato. Furthermore, the assembly of de novo transcript sequences represents an efficient and cost-effective method to recognize salt responsive genes in tomato wild relative S. chilense. Transcriptome studies in plants with salinity stress have been documented in many crops such as canola49, common bean50, ice plant51, Kentucky bluegrass52, wheat53 and desert poplar54. In this article, we carried out a comparative transcriptome analysis of S. lycopersicum and a wild relative of tomato, S. chilense based on the high-throughput Illumina HiSeq 2000 platform to deliver a comprehensive molecular data for identification of molecular markers, genes and pathway, and biochemical, molecular, and physiological basis of salt tolerance in S. chilense. These findings will provide valuable information for breeding salt tolerance in tomatoes.
Results and discussion
An overview of the de novo assembly of S. chilense transcriptome
Illumina HiSeq 2000 platform was used for RNASeq. A total of 88.37 million reads were generated in Chilense_Control, 101.23 million in Chilense_Treated, 101.50 million in DVRT-1_Control, and 100.23 million in DVRT-1_Treated. Over 96% of the reads had Phred-like quality scores at the Q30 level (error < 0.1%) (Table S1). We obtained 87.08 million clean reads in Chilense_Control, 100.01 million in Chilense_Treated, 100.08 million in DVRT-1_Control, and 98.82 million in DVRT-1_Treated, with 44.97% average GC content (Table S1). Further, all clean reads with high-quality regions were assembled into 514,747 unigenes with a maximum size of 46,034 bp, a minimum unigene size of 300 bp, 591.57 bp mean length, and a 578 bp of N50 value (Table S2). The unigene length, random distribution, classification, and annotation are presented in Fig. S1 and Fig. S2a to Fig. S2d.
Functional annotation and classification of transcriptome sequences
To perform the functional annotation of unigene dataset, we compared each unigene against the Genebank Nr, Viridiplantae, Gene Ontology (GO), euKaryotic Orthologous Groups (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Among the 514,747 unigenes, 201,803 (39.20%) were significantly annotated in the Genebank Nr database with the maximum hit rate against all four public databases (E value < 1.0 E−50). The annotated unigenes in Viridiplantae, GO, KOG, and KEGG databases were 160,452 (31%), 108,355 (21.05%), 126,937 (24%), and 7,923 (1.53%), respectively. Since the Whole Genome Sequence (WGS) of S. chilense was unavailable, annotation of unigenes was done by comparing the assembled transcriptome data to the public domain databases. Among the 514,747 assembled unigenes, 108,355 unigenes were assigned into 30 GO categories according to their sequence homology under three main categories, viz., cellular component (CC), biological process (BP) and molecular function (MF) each separately with 10 GO terms (Fig. S3). With respect to cellular components, integral component of membrane (41.18%) were the most dominant groups followed by nucleus (17.23%) and cytoplasm (11.94%) (Fig. S3). Among biological process, ATP binding process (31.23%), DNA binding (10.01%) and metal ion binding (9.82%) were highly represented (Fig. S3). Under molecular function term, transcription (28.82%), regulation of transcription (26.09%) and translation (20.85%) were most represented (Fig. S3). To further identify the biological pathways that were activated in S. chilense, 7,923 annotated unigenes were mapped to the reference canonical pathways in the KEGG and were assigned to 127 KEGG pathways. The most represented pathways were genetic information processing (56.39%), signaling and cellular processes (18.75%) and metabolism (17.56%) (Fig. S4 and Table S3). Furthermore, KOG classification indicated that 126,937 (24%) transcriptome sequences were gathered into 26 functional categories. Among the 25 groups, general function prediction represented the most enriched term (19.72%), which was followed by signal transduction mechanism (14.80%) and post-translational modification (Fig. S5 and Table S4).
Identification of SSR
By screening 514,747 transcriptome sequences, a total of 106,239 potential SSRs were identified, which distributed among 81,256 unigenes, and including 18,593 unigenes with more than one SSR. Among them, 8,784 SSRs were compound SSRs. The most abundant SSR type was mono-nucleotide (65.53%), followed by di-nucleotide (8.52%), tri-nucleotide (6.70%), tetra-nucleotide (0.59%), penta-nucleotide (0.09%), and hexa-nucleotide (0.06%) (Fig. S6, Table S5, and Table S6).
Identification of transcription factors families
Transcription factors (TF) play a significant role in plant development processes and regulate the expression of specific genes under different stress conditions55. Plant TFDB online tool was used for identification of potential transcription factor families. A total of 57 transcription factor families, containing 6,353 unigenes were identified (Table S7). Out of these, bHLH (8.99%) was found to be the most abundant TF family, followed by C2H2 (7.123%), MYB (6.02%), NAC (5.45%), ERF (5.05%), bZIP (4.71%), WRKY (4.63%), and MYB-related (4.61%) (Fig. 1).
Analysis of DEGs
Differentially expressed gene (DEGs) analysis is an important approach to identify the salinity tolerance responsive genes in S. chilense. Therefore, DEGs were identified from the RNA-seq data based on the criteria of fold change ≥ 2 for the comparisons salinity Chilense_Treated versus Chilense_Control, DVRT-1_Treated versus DVRT-1_Control, Chilense_Treated versus DVRT-1_Treated and an FDR < 0.05 (Tables 1, 2, 3, 4, 5). In studying expression pattern of DEGs in Chilense_Treated versus Chilense_Control , total of 67,293 DEGs (30,924 up-regulated and 36,369 down-regulated genes) were identified (Fig. S7a, Table S8, and Table S9), while 81,882 DEGs (42,426 up-regulated and 39,456 down-regulated genes) presented noteworthy changes in DVRT-1_Treated versus DVRT-1_Control comparison group (Fig. S7b, Table S8, and Table S10). In Chilense_Treated versus DVRT-1_Treated comparison group, 1,15, 983 DEGs (61,216 genes up-regulated and 54,767 down-regulated genes) were identified (Fig. S7c, Table S8, and Table S11). Heat map and cluster analysis revealed that the number of genes up-regulated under salinity in both S. chilense as well as DVRT-1 was higher compared to the down-regulated genes (Fig. S8).
Functional categorization of salinity-treated S. chilense and DVRT-1 DEGs
Blast2GO was used for GO term enrichment analysis for further understanding the biological function of the DEGs. The identified DEGs were highly abundant in 27 GO terms (p < 0.05), which showed that these DEGs were highly expressed in cellular processes in response to a stimulus metabolic process, localization, biological regulation and protein containing-complex (Fig. S9). Most of the upregulated DEGs under salt stress were abundant in catalytic activity, transcription regulator and molecular transducer. Whereas all the down-regulated DEGs were involved in binding, molecular function regulator and catalytic activity (Fig. S9). GO enrichment pathway analyses of these DEGs was conducted for pathway prediction. It was revealed that the DEGs were overrepresented in pathway terms [Wnt signaling (“Wingless-related integration site”), Epidermal growth factor (EGF) receptor signaling, Platelet-derived growth factor (PDGF) signaling], de novo purine biosynthesis, and ascorbate degradation (Fig. S10). Among these pathways, the “Wnt signaling pathway” was over-represented and has not been reported previously with regard to the salinity function. Nevertheless, presence of Wnt or Wnt-like signaling pathway in plants system is still unknown. Armadillo repeat proteins (ARM) play an important role in various developmental and stress signaling pathways in plants such as rice (Oryza sativa L.)56, Arabidopsis57, cotton (Gossypium hirsutum)58, and Physcomitrella patens59. Functional characteristics of ARM are similar to β-catenin, the key transcriptional modulator of Wnt signaling and Wnt-like signaling pathway in metazoan. For instance, in Arabidopsis SHAGGY-related protein kinase (ASK), 70% is similar to glycogen synthase kinase-3(GSK3) from mammals, the principal modulator of diverged biological functions in both plants and animals60. In plants, mostly GSKs participate in salt stress response and brassinosteroid signaling61. In consequence of these statements, SHAGGY-related protein kinase (ASK) and GSK3 were found to have higher expressions displayed in Chilense_Treated versus DVRT-1_Treated, which are the key genes responsible for the Wnt signaling (Table S9, S10 and S11). Therefore, our results suggest that Wnt signaling plays a crucial role in imparting salt tolerance in S. chilense. Pathway analyses of unigenes revealed that protein ubiquitination pathway was over-represented followed by glycan metabolism and carbohydrate degradation (Table S12, Fig. S11a to Fig. S11d).
REVIGO tools were used for summarizing the functional categorization of biological process (BP) and molecular function (MF) of top 15 GO terms enriched in DEGs of the S. chilense_Treated versus DVRT-1_Treated group according to the lowest p values62. For up-regulated genes in terms of biological process (BP), oxidation–reduction process, response to stress and protein metabolism were the considerably enriched GO terms (p < 0.05) (Fig. 2a). Similarly, other previous transcriptome analyses that are onsistent with our results have suggested that the above processes are associated with salt tolerance in several plants49,52,54. For down-regulated DEGs in terms of BP, enriched significant GO terms (p < 0.05) comprised mainly lipid biosynthesis process, transcription (DNA-template) and DNA repair (Fig. 2b). For the MF GO term with up-regulated genes, transferase activity and protein kinase activity was significantly enriched (Fig. 2c). The enriched augmented significant GO terms for down-regulated DEGs were contained within a catalytic activity and phosphoprotein phosphatase (Fig. 2d). For up-regulated DEGs, 93 GO BP terms and 47 GO MF terms were significantly enriched in Chilense_Treated versus DVRT-1_Treated group (Table S13). The outcome of our result suggest the possible mechanism for salinity tolerance through the up-regulation of numerous genes linked with many BP’s and MF’s in S. chilense under salinity.
Gene Ontology (GO) enrichment analysis of differentially expressed genes using REVIGO. The top fifteen GO terms enriched in Chilense_Treated_vs_DVRT-1_Treated DEGs, determined based on the lowest p values, were analyzed by REVIGO. (a,b) The enriched GO terms from the REVIGO analysis for up-regulated (a) and down-regulated (b) genes in the Chilense_Treated_vs_DVRT-1_Treated comparison group for Biological Process. (c,d) The enriched GO terms from the REVIGO analysis for up-regulated (c) and down-regulated (d) genes in the Chilense_Treated_vs_DVRT-1_Treated comparison group for Molecular Function. Circles in closer proximity have more closely related GO terms. The size of the circles indicates the number of child GO terms. The color of the circle represents the significance of the enriched GO terms.
Identification of key salt tolerance-related genes
In the present study, numerous salt-responsive genes were identified that could positively contribute to salt tolerance in S. chilense. It could be predicted that responsive genes for salt-tolerance displaying differential pattern of expression in the different comparison groups might be playing a key role in salinity tolerance. Subsequently, the overlay among DEGs from the different comparison groups were studied in detail, particularly for the genes which are up-regulated (Tables 1, 2, 3, 4, 5). Validation and confirmation of RNA-seq data were done by qPCR of selected DEGs and gene numbers (Q1–Q69) correspond to the gene labels in Tables 1, 2, 3, 4 and 5 (Fig. 3, Fig. S12 to Fig. S13). Most of the up-regulated genes and major DEGs actively participated in proline and arginine metabolism (Table 1, Fig. 3, Q1 to Q4), oxidoreductase activity (Table 1, Fig. 3, Q5 to Q7), hormone metabolism (Table 1, Fig. 3, Q8 to Q14), ROSscavenging system (Table 2, Fig. 3, Q15 to Q21), signaling regulation (Table 2, Fig. 3, Q22 and Fig. S12, Q23 to Q34), transporters (Table 3, Fig. S12, Q35 to Q43 and Fig. S13, Q44 to Q48), osmotic regulation (Table 4, Fig. S13, Q49 to Q52), defense and stress response (Table 4, Fig. S13, Q53 to Q56), homeostasis (Table 4, Fig. S13, Q57), and transcription factors (Table 5, Fig. S13, Q58 to Q69). Furthermore, genes involved in secondary metabolite biosynthetic process, regulation of systemic acquired resistance, glutathione biosynthesis, glycan metabolism, starch biosynthesis, carbohydrate metabolism, glutathione metabolism, and flavonoid metabolism were also significantly up-regulated (Tables 1, 2, 3, 4 and 5). In our results, we reported down-regulation of polyubiqutin gene in S. chilense as well as cultivated DVRT-1. However, this down regulation was more pronounced in S. chilense compared to DVRT-1. In one of the studies, Krishnamurthy et al.63 reported down regulation of polyubiqutin gene during salt stress in halophyte Avicennia officinalis. Likewise, we found significant down-regulation of gene encoding pentatricopeptide repeat-containing protein in S. chilense compared to its counterparts DVRT-1. In this context, Wang et al.64 demonstrated that gene encoding pentatricopeptide repeat-containing protein was down-regulated to provide defense against salinity stress in Eucommia ulmoides.
Expression patterns of selected candidate genes related with amine, polyamine metabolism, oxidation–reduction, hormone, ROS scavenging and signaling transduction in Chilense and DVRT-1 with and without salt treatment determined by RNA-seq and qPCR. The RNA-seq values represent the ratio of the expression level in chilense to the expression level in DVRT-1. Bars with distinct letters are significantly different at p ≤ 0.05 applying the DMRT test.
Most of the halophytes have been reported to accrue the greater amount of solutes like glycine betaine, proline, sugars, and polyols against osmotic stress during the salinity stress65. In support of this, we detected that the expression of those gene groups which affect the synthesis and metabolites of salt-responsive solutes were significantly higher, for example, glycine betaine biosynthesis encoded by genes betaine aldehyde dehydrogenase (BADH) and choline monooxygenase (CMO)66, and trehalose biosynthesis encoded by genes trehalose 6-phosphate phosphatase (TPPA) and Hexokinase1 (HXK1)67 in mangrove (Avicennia officinalis) treated with salt. Greater accumulation of CMO and BADH has been reported earlier in many other species such as sugar beet, Atriplex, Suaeda, Halogeton, etc.68,69,70,71. In the present study too, the expression of BADH, CMO, trehalose 6-phosphate phosphatase (TPPA), and Pyrroline-5-carboxylate reductase were up-regulated in S. Chilense (Table 1).
Among genes related with the metabolism of proline and arginine, transcript levels of polyamine oxidase (PAO) genes that are directly involved in proline metabolism were augmented in S. Chilense (Table 1). The qPCR analysis revealed that the expression patterns of these genes were similar to the transcript levels of the transcriptome data (Fig. 3, Q1–Q4). The transcript levels of the arginine decarboxylase (ADC) encoding the genes regulating the synthesis of putrescine were also identified to have a higher expression in S. Chilense (Table 1). Polyamine oxidase and arginine decarboxylase genes play important roles in polyamine and arginine metabolism, and are essential during different environmental stresses72,73,74,75. Polyamine oxidase has been well-documented in grapevine for drought tolerance improvement76. Putrescine plays an important role to minimize the harmful effect of salinity in soybean roots by reducing the oxidative damage34. Additionally, numerous studies have depicted that the polyamine catabolism and synthesis are important factors for mitigating the adverse-effect of salinity77,78. In our findings, the transcripts expression level of significant genes in polyamine metabolism were augmented in S. Chilense, indicating that the polyamine modulation is essential for salt tolerance in S. chilense.
Oxidation–reduction processes are another of important factors crucial for salinity tolerance in many plants. GO enrichment analysis revealed that many up-regulated genes were involved in the oxidation–reduction process, which contained the genes mainly associated with glutaredoxin, cytochrome P450 (CYP) and ascorbate oxidoreductase, with relatively higher expression in S. chilense (Table 1). Specifically, seven genes that participate in oxidoreductase activity through encoding cytochrome P450 were up-regulated in the S. chilense (Table 1). Expression patterns of these genes in qPCR analysis were similar to RNASeq transcript data (Fig. 3, Q5–Q7). Cytochrome P450 regulates the activation of molecular oxygen that catalyzes the reaction of bio-oxidation of numerous substrates and controls the metabolic processes of the plant in responses to stress79. Higher expression of CYP94 genes improves jasmonate response which is reported to increase salt tolerance in rice80. L-ascorbate oxidase genes which participate in the recycling of ascorbate had higher expression recoded in S. Chilense (Table 1). Ascorbate is an essential component for detoxification of cellular H2O2 through catalytic Halliwell-Asada cycle that regulates the reduction of H2O2 to water and thus play critical roles in plant survival against different stresses81. Overexpression of glutaredoxin in tomato plants against salt, drought, and oxidative stresses improves tolerance to abiotic stress82. In terms of the entire stress-tolerances scenario, it was concluded that the oxidoreductase process actively participates in ROS scavenging and is important for sustaining homeostasis of oxidation–reduction83, and therefore protects the plants from adverse effects of salinity.
Mechanism of salt tolerance in S. chilense
We reasoned that phytohormones, Ca2+, ROS, and specific TFs may play critical role in the regulation of different signaling pathways under salt stress. Therefore, total DEGs identified in our study which are expected to be associated with or directly involved in cytokinin, ethylene, auxin ABA and gibberellin signaling pathways were critically analyzed for better understanding of important signaling modules in higher salt stress environments.
Role of phytohormone signaling
Hormones like cytokinins, ethylene, jasmonate, and abscisic acid also play a important role in the salinity tolerance regulation mechanism in plants84. In term of hormone-related signaling, genes corresponding to jasmonate, ethylene, abscisic acid, auxin, gibberellin, and cytokinins were found to have greater expression in salt-treated S. chilense, which may contribute to improved salt-tolerance (Table 1, Fig. 3, Q8 to Q14). Improved salinity tolerance through higher expression of jasmonate content by allene oxide cyclase gene has been reported in wheat and Arabidopsis85. In consequence of these results, allene oxide synthase 2-like, three lipoxygenase family genes, and one 12-oxophytodienoate reductase gene were found to have augmented expressions displayed in salt-treated S. chilense, which are the key genes responsible for the jasmonic acid biosynthesis (Table 1). Similar results are also found in our qPCR analysis that 12-oxophytodienoate reductase, lipoxygenase family genes and allene oxide synthase 2-like gene had considerably greater expressions in the salt-treated S. chilense compared to salt-treated DVRT-1 (Fig. 3, Q8, Q13A, Q13B and Q14). Possibly, the augmented expression levels of jasmonate biosynthetic genes under high salinity may play as one of the important factots for enhanced salinity tolerance in S. chilense. Makhlouf et al. described the critical role of ethylene response factor genes which are probably related with salinity-tolerance in wheat86. Similarly, ethylene response factor genes have also been reported to play a crucial role in response to high salt concentration in different plants84,87,88. Regulation and biosynthesis of ethylene by 1-aminocyclopropane-1-carboxylate oxidase gene were up-regulated in the Chilense_Treated group (Table 1), which was also confirmed by qPCR expression patterns (Fig. 3, Q9). Additionally, the expression pattern of numerous genes related with ethylene biosynthesis like serine hydroxy methyltransferase (SHMT), S-Adenosyl methionine synthetase (SAM2), and methionine synthase were detected to be greater in the Chilense_Treated group in both the qRT-PCR and RNASeq results (Table 2, Fig. S12, Q32–Q34). Moreover, ethylene signaling encoded by hexokinase89 and proline synthesis encoded by glutamate synthase90,91 are well-identified genes for salt tolerance (Table 2). Alterations in the expression pattern of ethylene-associated genes suggested that ethylene may act as an important factor for tolerance to salinity in S. chilense. Hence, we theorize that the ethylene signaling plays an important role for salt tolerance in S. chilense under high salinity. In the present study, many genes associated with auxin biosynthesis and metabolism such as 14-3-3 protein zeta were up-regulated (Table 2, Fig. S12, Q28). This strongly indicates that the auxin signaling was highly involved in salinity tolerance in S. chilense. Besides, genes involved in biosynthesis or regulation of abscisic acid, cytokinin, auxin, and gibberellin showed higher expression levels in salt treated S. chilense.
Role of ROS
Several genes that regulate the activity of enzymes and are involved in detoxification and scavenging of reactive oxygen species (ROS) during salt stress were found up-regulated in S. chilense, in support of earlier reports92,93 (Table 2 Fig. 3, Q15 to Q21). These contain superoxide dismutase, catalase, peroxidases, glutathione peroxidase, glutaredoxin, and thioredoxin reductase. Up-regulation of antioxidant genes revealed that the severe oxidative stress is prompted by salt stress, and thus scavenging and ROS detoxifying enzymes are responsible for tolerance and metabolic adaptation with respect to salinity in S. chilense. Significant up-regulation of antioxidants is also noticed in various crops under salt stress like jatropha, calendula, tomato, pea, maize seedlings, and bean seedlings94,95,96,97,98,99,100,101,102.
Role of Ca2+signaling
Various types of signal transduction genes were up-regulated in S. chilense (Table 2). These transcript results were validated by the qPCR analysis of selected genes (Fig. 3, Q22 and Fig. S12, Q23–Q34). Genes encoding signal transduction function such as CBL-interacting protein kinases (CIPK), protein histidine kinase, protein serine/threonine kinase, calmodulin, calcium/calmodulin-dependent protein kinase along with other genes related to signal transduction were up-regulated in S. chilense (Table 2). In Arabidopsis, the network cascade of CBL/CIPK has been well-known for regulation of Na+ efflux transporter SOS1 activity17,103,104. Numerous genes associated with CIPK in many plants have already been established for their salt tolerance function18,104,105, and it is assumed that the higher expression level of CIPK genes endorses greater tolerance under salinity environment in S. chilense.
Under different abiotic stresses, the most vital second messenger for plant signaling networks is calcium. Various stress-stimuli (such as salt stress) results in an upsurge of Ca2+ levels in the plant cytosol within few seconds by means of Ca2+ pumps and transporters63. In our study, many genes associated with the signaling of Ca2+ were up-regulated (Tables 2, 3). Ca2+/H+ exchangers (CAXs), cyclic nucleotide-gated channels (CNGCs), and calcium-transporting ATPases (ACAs) were significantly up-regulated in S. Chilense, which could be the main factor of the Ca2+ fluxes during salinity (Table 3, Fig. S12, Q39 and Q43). Cyclic nucleotide-gated channels (CNGCs) are accountable for the uptake of Ca2+, Na+, and K+, whereas Sodium/hydrogen exchanger or NHXs are known to be involved in the compartmentalization of K+, Na+, and pH homeostasis. All these functions are regulated by pH gradient produced by V-ATPases106. K+ transporters also play an important role in ionic balance process which is otherwise hampered due to salinity stress107. ABC transporters are concerned in several functions comprising transport of Na+, osmolytes, heavy metals, auxin and fatty acids108,109,110. While the major role of cyclic nucleotide-gated channels during salt stresses is to influx the Ca2+ ion across the plasma membrane, the Ca2+/H+ exchangers and calcium-transporting ATPases perform efflux process of Ca2+ across the plasma membrane and tonoplast, correspondingly. As a result of the higher level of Na+/Cl-, the concentration level of Ca2+ ion is increased and sensed immediately by the calcium sensors like CBLs, CIPKs, CAM kinases, and SOS3103,111, all of these being significantly up-regulated in S. Chilense (Table 2, Fig. S12, Q26, Q27, Q29A, Q29B and Q31). Further, Ser/Thr protein kinases (CIPKs/SOS2) interact with SOS3 resulting in formation of a specific SOS3-SOS2 complex that is adequate to trigger several downstream targets against salinity112. The NHX1 and Na+/H+ antiporters SOS1, triggered by the activated SOS3–SOS2 complex initiates efflux of Na+ across the plasma membrane and compartmentalization of Na+ into vacuoles at the same time112,113. Additionally, HKT is known to prevent the entry of Na+ in the roots, thus providing salt tolerance in plants. In our study, it was found that both HKT and Na+/H+ antiporter activities were up-regulated in S. Chilense (Table 3, Fig. S13, Q48); whereas many other regulatory components were also highly up-regulated in S. Chilense (Table 3, Fig. S12, Q35 to Q43 and Fig. S13, Q44 to Q48). Besides these, calcium ion binding proteins encoded by calmodulin genes and Ca+/H+ exchangers also proved their significant roles in salt tolerance in S. chilense (Tables 2, 3). Comprehensively, our results indicate that signaling of Ca2+ play a crucial role in salt tolerance in S. chilense in addition to the role of salt-responsive phytohormones.
Role of transcription factors
Most of the biological processes, specifically stress response is under direct influence by transcriptional gene expression114. In the current study, a total of 6,353 TFs were identified that grouped into 57 TFs family (Table S7). In our study, more than twelve different types of TFs and salt-induced genes encoded by their-respective proteins were recorded in higher expression in salt-treated S. chilense compared to the salt-treated DVRT-1 plants (Table 5), advising that these TFs may contribute to more tolerance against salinity conditions in S. chilense. The TFs bZIP, MYBs, bHLH, C2H2-Dof, G2-like, and AP2-EREBP are well-known to play a major role in salt-stress responses of common bean, Oryza sativa, Raphanus sativus, A. thaliana, and Populus trichocarpa22,23,24,115. In our findings, TFs from the Dof zinc finger protein, C3HC4-type zinc RING finger, WRKY, bHLH, TCP transcription factor 23, NAC, GATA, ARF, ERF, bZIP, and MYB families were significantly up-regulated in S. chilense, proposing that these TFs must be regulating salt-stress response in S. chilense (Table 5, Fig. S13, Q58 to Q69).
Role of bHLH TF in salinity response has been reported earlier in other plant species116. Up-regulation of bHLHs TFs with diverse peroxidase antioxidants genes in the present study proposes their possible participation in peroxidase-mediated ROS detoxifying-regulation under salt treatment in S. chilense. Involvement of bHLH TFs in SOS1 regulation through genes encoding Na+/H+ plasma membrane antiporter is important for salt tolerance and has been documented in other plants117. Shi and Chan 118 reported that the expression of AtZAT6 and C2H2 zinc-finger protein can be triggered by salt, drought, and cold stresses in Arabidopsis. In compliance with this, a total of 11 different zinc-finger TFs were up-regulated in our experiments suggesting the possible role of zinc-finger proteins in salt tolerance in S. chilense. MYB TFs are involved in secondary metabolism, abiotic stress tolerance, hormone signal transduction, and disease resistance in plants119. MYB59, MYB48-like, MYBR1, MYB76, and MYB12 were found to have higher expression under salt stress (Table 5), and could play a crucial role in the salt tolerance in S. chilense. Besides these, WRKY TFs also actively participate in the regulation of abiotic stress process and have been reported in several crops. In tomato, SlWRKY3 is significantly up-regulated by the induction of drought and salt stress120. In soybean, 25 WRKY genes are involved in drought and high salt stress treatment121. Similar to these results, three WRKY TFs , viz., WRKY1, WRKY3 and WRKY72 displayed observably higher expression during salt stress in our study (Table 5), justifying the significant role of WRKY TFs in salt tolerance of S. chilense. Earlier studies have successfully established the correlation among the DEGs from transcriptome and qPCR data49,122. The validation of selected genes through qPCR revealed the similar expression patterns corresponding to the DEGs from transcriptome data, but the fold changes were not accurately the same.
Overall, the findings of our experiments directly advocate that different genes, which are actively-involved in ion homeostasis, phytohormone, oxidation–reduction, ROS scavenging, detoxification, signaling transduction, transporters, osmotic regulation, defense and stress response, and other metabolic processes are significantly up-regulated, and play important role in salt tolerance of S. chilense. The functionality of salt-responsive genes identified in the present study is required to be ascertained for their further validation through different experimentation. With the support of comparative transcriptomic approach, strategic salt tolerance-responsive genes in S. chilense were identified, and the chronological expression patterns of selected genes were validated through qRT-PCR. In addition to Ca2+ signaling, phytohormones and transporters are well known to portray crucial roles in plants salt tolerance123. The vacuolar ATPases and NHXs also play important roles in the homeostasis of Na+/K+ in S. chilense, with their functions much similar in other plants124. In the same way, up-regulation of numerous genes related to signaling pathways emphasized their significance and importance in S. chilense.
Conclusion
In this study, a comparative transcriptomic analysis of S. chilense and cultivated tomato, in response to 500 mM NaCl was carried out to identify the various genes and pathways associated with salinity stress, as well as to deliver a valuable catalog of RNA-Seq difference caused by salinity. Our results have revealed the interactions among the genes involved in Ca2+, auxin, and ethylene mediated signaling network in response to salt-stress. This information will provide worthy genomics and molecular knowledge for future research on salt tolerance in S. chilense and other related species of tomato.
Materials and methods
Plant materials, growth conditions, and salt treatment
Two tomato species, viz., cultivated tomato (S. lycopersicum L.) cv. DVRT-1/Kashi Amrit, and a wild relative of tomato (S. chilense L.) were used in this experimental study. Seeds of S. chilense (LA1972) were provided by Cranfield University, MK 43 0AL, United Kingdom. Seeds of both tomato genotypes were sown and raised inside a greenhouse (25.3521° N, 82.9502° E) at ICAR-Indian Institute of Vegetable Research, Varanasi, India. Tomato is a grown as a transplanted crop and 25–30 days old seed lings are transplanted in the main field. Tomato plants are more likely to be exposed to salinity in the main field than in nursery. Therfore, 1-month-old seedlings were transplanted into small pots (one pot/plant) filled with soil, mixture of vermiculite-perlite (1:3), and farmyard manure (5:1) under greenhouse conditions: 26 °C, 16 h for day, and 15 °C, 8 h for night, and 55% to 65% relative humidity. In order to reduce plasmolysis caused by the osmotic shock, NaCl was gradually increased at a rate of 50 mM each day until the desired concentration (500 mM) was reached (Fig. S14). A control treatment (0) was carried out without NaCl solution with electrical conductivity (EC) 3.8 dSm–1. NaCl treated seedlings were carried out with 500 mM NaCl solution with EC 26.8 dSm–1. 21 days after reaching 500 mM NaCl, samples were collected for RNA sequencing from the NaCl treated and control plants viz. S. chilense and S. lycopersicum plants. For each treatment, 9 seedlings (three plants from three replicates) each from the control and treated were harvested after 21 days of salt treatment.
RNA extraction and cDNA library preparation
Tissue samples from three plants for each treatment were pooled and ground to fine powder to create one pooled sample. One sample from each treatment was used for Illumina sequencing. Further, two technical replicates for each treatment derived from pool sample were used for total RNA isolation and qRT-PCR analysis. 200 mg ground leaf tissue was used to isolate total RNA using RNeasy Plant Mini Kit (Qiagen, USA) as per the protocol suggested by the manufacturer. RNA purification kit and DNase I (NEB, USA) were used for removing DNA contaminations from the total RNA. The purity and quality of RNA samples were measured on agarose gel electrophoresis and in a Nano-spectrophotometer (Implen, USA). Integrity of RNA was checked using the Nano 6000 Assay Kit with the Agilent 2100 Bioanalyzer (Agilent Technologies, USA). RNA concentration was evaluated using a Qubit RNA Assay Kit with Qubit 2.0 Fluorometer (Life Technologies, USA). RNA Library Prep Kit NEBNext Ultra for Illumina (NEB, USA) was used for the construction of sequencing libraries following the recommendations of the manufacturer. Further, Oligo(dT)-attached magnetic beads were used for mRNA purification. The cDNA library was prepared after fragmentation, and hybridization process was completed with ligation of NEBNext hairpin loop adaptors (Illumina, USA). AMPure XP system (Beckman Coulter Life Science, USA) was used in PCR products purification, and cDNA library quality was checked on an Agilent 2100 Bioanalyzer. Finally, a total of four cDNA libraries [For each individual treatments (salt treated and control)] were successfully assembled.
Sequencing and transcriptome de novo assembly
To identify and gain knowledge on the genetic and molecular mechanisms of salt tolerance in tomato species, four cDNA libraries were constructed. It included one assembly for each individual treatments of S. chilense accession and cultivated tomato cultivar (Chilense_Control, Chilense_Treated, DVRT-1_Control, and DVRT-1_Treated). Thus obtained cDNA libraries were deep sequenced using Illumina HiSeq 2000 sequencer to produce paired-end reads (150 bp). Illumina data processing pipeline125 was used to check the quality of raw reads. The rawreads were pre-processed to get the high-quality clean reads. The raw reads were filtered by removing reads with adaptor sequences, reads containing duplicated sequences, reads covering more than 10% ambiguous base reads (“N”), and low quality reads with more than 50% of low-quality bases (Q-value ≤ 10). All downstream assembly analyses were done on processed high-quality reads. De novo assembled contigs were generated from the clean reads using a de novo transcript assembly tool Trinity126. Redundancy was removed by TGICL software127, and thus the sequences were assembled into a non-redundant single contig. The longest contigs of chimeric and redundant transcripts were referred as unigenes (viz. 25 k-mer lengths), and were exposed to the downstream coding sequence (CDS) and functional annotation prediction. Assembled unigene sequences were aligned using following databases: BLASTx (E-value ≤ 1E-5) (https://www.ncbi.nlm.nih.gov/BLAST/) contrary to NCBI database of non-redundant protein (nr) (https://www.ncbi.nlm.nih.gov)128, the KEGG database (https://www.genome.jp/kegg)129,130, the Viridiplantae, and the KOG/COG database (https://www.ncbi.nlm.nih.gov/COG)131. ESTScan132 was used to align sequences for those unigenes that could not be aligned to any of the above databases. The RNA-Seq raw data generated from this study was submitted to the Sequence Read Archive (SRA), National Centre for Biotechnology Information (NCBI) website with the accession numbers PRJNA559982 and PRJNA560126.
Data analysis
To assign the gene functions of identified unigenes, sequences were explored in the aforesaid public databases with BLASTx program. Proteins with highest sequence similarity to the S. chilense and S. lycopersicum unigenes along with their functional annotations were retrieved. Gene Ontology (GO) annotation was completed after annotation of nr using software Blast2GO133. PANTHER14.1 tool was used to carry out functional classification of the unigenes and to assign the GO distribution of gene functions at the broad level134. The unigenes were further aligned using KEGG and COG/KOG annotation to get pathway information for an identified orthologous gene. For unigenes mapping, the clean read sequences were mapped deploying Bowtie version 2.1.0135 and Tophat version 2.0.8136 on tomato genome SL 2.5 (version 2.50) https://solgenomics.net/. Further, quantification of the reads abundance per each gene (exon level) was performed using AIR (https://transcriptomics.sequentiabiotech.com/) and were obtained through iTAG gene annotation (version 2.5). Gene expression analysis was carried out using reads per kilobase per million reads (RPKM) method137. Differential expression analysis was performed using the DESeq R package (1.18.0). False discovery rate (FDR) ≤ 0.001, the absolute value of log2Ratio ≥ 2 and p-value ≤ 0.001 was used as the threshold to judge the significance of differential gene expression in salt-treated and untreated samples of both S. chilense and S. lycopersicum138. For GO enrichment and pathway analysis, all the DEGs were mapped in GO and KEGG databases (https://www.geneontology.org/) and further analysis was completed with GOseq R package. Enrichment analysis of KEGG pathways of DEGs was done by KOBAS software139. DEGs sequences were further aligned to the published microarray/transcriptome data to categorize the important genes related to salt-tolerance in S. chilense and S. lycopersicum.
Identification of transcription factor families
The unigenes sequence was analyzed using online tool PlantTFcat (https://plantgrn.noble.org/PlantTFcat)140 to identify the putative TFs families in the public domain transcriptome databases.
SSRs identification
The identification and distribution of various types of simple sequence repeats (SSRs) were analyzed using the MIcroSAtellite tool (MISA version 1.0; https://pgrc.ipkgatersleben.de/misa/misa.html)141.
Quantitative real-time PCR (qRT-PCR) analysis
For analysis of quantitative qRT-PCR, the first-strand cDNA was synthesized from one microgram of total RNA with iScript cDNA synthesis kit (Bio-Rad Laboratories, USA) according to manufacturer’s protocol. SsoFast EvaGreen Supermix (BioRad Laboratories, USA) was used to study expression analysis of total 69 salt stress related genes using qRT-PCR following the manufacturer's instructions with an iQ5 thermal cycler (BioRad Laboratories, USA). The primers used for qRT-PCR are detailed in supplementary Table S14. Quantitative PCR reactions were performed in 8 strips optical flat PCR tube and each reaction mixture containing 10 μl of SsoFast EvaGreen Supermix (2×), 1 μl of specific forward and reverse primers (200 nM), 2 μl of the reverse-transcribed cDNA template (25 ng), and 6 μl ddH2O. The qRT PCR conditions were performed with following steps: 95 °C for 10 min, followed by 45 cycles at 95 °C for 15 s, at 55 °C to 60 °C (primer melting temperature) for the 30 s, at 72 °C for 30 s and finally one cycle at 72 °C for 5 min. Every reaction was repeated three times and the relative level of gene expression quantification was calculated using the 2−ΔΔCT method131. NormFinder software was used for analysing the expression stability check values of reference genes, which is based on variance analysis to calculate the stable value of each gene142. According to the stability values of reference genes calculated by NormFinder (Table S14), Actin (0.142) was found the most stable genes under salinity stress condition. Constitutively expressed actin gene was used as internal control for normalization of the Ct value.
References
Dang, Z.-H. et al. Transcriptomic profiling of the salt-stress response in the wild recretohalophyte Reaumuria trigyna. BMC Genom. 14, 29 (2013).
Xin, S. et al. Expression of tomato SlTIP2; 2 enhances the tolerance to salt stress in the transgenic Arabidopsis and interacts with target proteins. J. Plant. Res. 127, 695–708 (2014).
Munns, R. & Tester, M. Mechanisms of salinity tolerance. Annu. Rev. Plant Biol. 59, 651–681 (2008).
Ruan, C.-J., da Silva, J. A. T., Mopper, S., Qin, P. & Lutts, S. Halophyte improvement for a salinized world. Crit. Rev. Plant Sci. 29, 329–359 (2010).
Qadir, M. et al. Productivity enhancement of salt-affected environments through crop diversification. Land Degrad. Dev. 19, 429–453 (2008).
Flowers, T. J. et al. Salt sensitivity in chickpea. Plant Cell Environ. 33, 490–509 (2010).
Flowers, T. J. & Colmer, T. D. Salinity tolerance in halophytes. New Phytol. 179, 945–963 (2008).
Gupta, B. & Huang, B. Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. Int. J. Genom. 2014, 701596 (2014).
Roy, S. J., Negrão, S. & Tester, M. Salt resistant crop plants. Curr. Opin. Biotechnol. 26, 115–124 (2014).
Magnan, F. et al. Mutations in AtCML9, a calmodulin-like protein from Arabidopsis thaliana, alter plant responses to abiotic stress and abscisic acid. Plant J. 56, 575–589 (2008).
Zhang, H. et al. Mechanisms of plant salt response: insights from proteomics. J. Proteome Res. 11, 49–67 (2011).
Deinlein, U. et al. Plant salt-tolerance mechanisms. Trends Plant Sci. 19, 371–379 (2014).
Ludwig, A. A., Romeis, T. & Jones, J. D. CDPK-mediated signalling pathways: specificity and cross-talk. J. Exp. Bot. 55, 181–188 (2004).
Nakagami, H., Pitzschke, A. & Hirt, H. Emerging MAP kinase pathways in plant stress signalling. Trends Plant Sci. 10, 339–346 (2005).
Shi, H., Ishitani, M., Kim, C. & Zhu, J.-K. The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter. Proc. Natl. Acad. Sci. 97, 6896–6901 (2000).
Mahajan, S., Pandey, G. K. & Tuteja, N. Calcium-and salt-stress signaling in plants: shedding light on SOS pathway. Arch. Biochem. Biophys. 471, 146–158 (2008).
Kudla, J., Batistič, O. & Hashimoto, K. Calcium signals: the lead currency of plant information processing. Plant Cell 22, 541–563 (2010).
Zhang, H., Lv, F., Han, X., Xia, X. & Yin, W. The calcium sensor PeCBL1, interacting with PeCIPK24/25 and PeCIPK26, regulates Na+/K+ homeostasis in populus euphratica. Plant Cell Rep. 32, 611–621 (2013).
Sun, J. et al. NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar species. Plant Physiol. 149, 1141–1153 (2009).
Horie, T., Hauser, F. & Schroeder, J. I. HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plants. Trends Plant Sci. 14, 660–668 (2009).
Wang, C. et al. Ectopic expression of a cytochrome P450 monooxygenase gene PtCYP714A3 from populus trichocarpa reduces shoot growth and improves tolerance to salt stress in transgenic rice. Plant Biotechnol. J. 14, 1838–1851 (2016).
Sharma, M. K. et al. Identification, phylogeny, and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato. Mol. Genet. Genom. 284, 455–475 (2010).
Kavas, M. et al. Genome-wide characterization and expression analysis of common bean bHLH transcription factors in response to excess salt concentration. Mol. Genet. Genom. 291, 129–143 (2016).
Sun, X. et al. Transcriptome-based gene expression profiling identifies differentially expressed genes critical for salt stress response in radish (Raphanus sativus L.). Plant Cell Rep. 35, 329–346 (2016).
Sanders, D., Pelloux, J., Brownlee, C. & Harper, J. F. Calcium at the crossroads of signaling. Plant Cell 14, S401–S417 (2002).
Cheng, S.-H., Willmann, M. R., Chen, H.-C. & Sheen, J. Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol. 129, 469–485 (2002).
Harper, J. F., Breton, G. & Harmon, A. Decoding Ca2+ signals through plant protein kinases. Annu. Rev. Plant Biol. 55, 263–288 (2004).
Bouché, N., Yellin, A., Snedden, W. A. & Fromm, H. Plant-specific calmodulin-binding proteins. Annu. Rev. Plant Biol. 56, 435–466 (2005).
Yoo, J. H. et al. Direct interaction of a divergent CaM isoform and the transcription factor, MYB2, enhances salt tolerance in Arabidopsis. J. Biol. Chem. 280, 3697–3706 (2005).
Pandey, N. et al. CAMTA 1 regulates drought responses in Arabidopsis thaliana. BMC Genom. 14, 216 (2013).
Weng, H., Yoo, C. Y., Gosney, M. J., Hasegawa, P. M. & Mickelbart, M. V. Poplar GTL1 is a Ca2+/calmodulin-binding transcription factor that functions in plant water use efficiency and drought tolerance. PLoS ONE 7, e32925 (2012).
Astolfi, S. & Zuchi, S. Adequate S supply protects barley plants from adverse effects of salinity stress by increasing thiol contents. Acta Physiol. Plant. 35, 175–181 (2013).
Fatma, M., Masood, A., Per, T. S., Rasheed, F. & Khan, N. A. Interplay between nitric oxide and sulfur assimilation in salt tolerance in plants. Crop J. 4, 153–161 (2016).
Zhang, G.-W., Xu, S. C., Hu, Q.-Z., Mao, W.-H. & Gong, Y.-M. Putrescine plays a positive role in salt-tolerance mechanisms by reducing oxidative damage in roots of vegetable soybean. J. Integr. Agric. 13, 349–357 (2014).
Chen, M., Chen, J., Fang, J., Guo, Z. & Lu, S. Down-regulation of S-adenosylmethionine decarboxylase genes results in reduced plant length, pollen viability, and abiotic stress tolerance. Plant Cell Tissue Organ Cult. (PCTOC) 116, 311–322 (2014).
Zahedi, S. M., Abdelrahman, M., Hosseini, M. S., Hoveizeh, N. F. & Tran, L.-S.P. Alleviation of the effect of salinity on growth and yield of strawberry by foliar spray of selenium-nanoparticles. Environ. Pollut. 253, 246–258 (2019).
Foolad, M. R. Genome mapping and molecular breeding of tomato. Int. J. Plant Genom. 2007, 64358 (2007).
Rao, A. V. & Agarwal, S. Role of antioxidant lycopene in cancer and heart disease. J. Am. Coll. Nutr. 19, 563–569 (2000).
Flowers, T. J. & Colmer, T. D. Plant salt tolerance: adaptations in halophytes. Ann. Bot. 115, 327–331 (2015).
Miller, J. & Tanksley, S. RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor. Appl. Genet. 80, 437–448 (1990).
Chetelat, R. T., Pertuzé, R. A., Faúndez, L., Graham, E. B. & Jones, C. M. Distribution, ecology and reproductive biology of wild tomatoes and related nightshades from the Atacama Desert region of northern Chile. Euphytica 167, 77–93 (2009).
Martínez, J. P. et al. Salt stress differently affects growth, water status and antioxidant enzyme activities in Solanum lycopersicum and its wild relative Solanum chilense. Aust. J. Bot. 62, 359–368 (2014).
Tapia, G., Méndez, J. & Inostroza, L. Different combinations of morpho-physiological traits are responsible for tolerance to drought in wild tomatoes Solanum chilense and Solanum peruvianum. Plant Biol. 18, 406–416 (2016).
Kashyap, S. et al. Transcriptional regulation-mediating ROS homeostasis and physio-biochemical changes in wild tomato (Solanum chilense) and cultivated tomato (Solanum lycopersicum) under high salinity. Saudi J. Biol. Sci. 27, 1999–2009 (2020).
Salazar, M., González, E., Casaretto, J. A., Casacuberta, J. M. & Ruiz-Lara, S. The promoter of the TLC1. 1 retrotransposon from Solanum chilense is activated by multiple stress-related signaling molecules. Plant Cell Rep. 26, 1861–1868 (2007).
Martinez, J.-P. et al. Effects of saline water on water status, yield and fruit quality of wild (Solanum chilense) and domesticated (Solanum lycopersicum var. Cerasiforme) tomatoes. Exp. Agric. 48, 573–586 (2012).
Mardis, E. R. The impact of next-generation sequencing technology on genetics. Trends Genet. 24, 133–141 (2008).
Bräutigam, A. & Gowik, U. What can next generation sequencing do for you? Next generation sequencing as a valuable tool in plant research. Plant Biol. 12, 831–841 (2010).
Long, W., Zou, X. & Zhang, X. Transcriptome analysis of canola (Brassica napus) under salt stress at the germination stage. PLoS ONE 10, e0116217 (2015).
Büyük, İ et al. Genome-wide identification of salinity responsive HSP70s in common bean. Mol. Biol. Rep. 43, 1251–1266 (2016).
Tsukagoshi, H. et al. RNA-seq analysis of the response of the halophyte, Mesembryanthemum crystallinum (ice plant) to high salinity. PLoS ONE 10, e0118339 (2015).
Bushman, B. S., Amundsen, K. L., Warnke, S. E., Robins, J. G. & Johnson, P. G. Transcriptome profiling of Kentucky bluegrass (Poa pratensis L.) accessions in response to salt stress. BMC Genom. 17, 48 (2016).
Goyal, E. et al. Transcriptome profiling of the salt-stress response in Triticum aestivum cv Kharchia Local. Sci. Rep. 6, 27752 (2016).
Zhang, J. et al. Transcriptome dynamics of a desert poplar (Populus pruinosa) in response to continuous salinity stress. Plant Cell Rep. 33, 1565–1579 (2014).
Nakashima, K., Ito, Y. & Yamaguchi-Shinozaki, K. Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol. 149, 88–95 (2009).
Sharma, M. et al. Comprehensive expression analysis of rice Armadillo gene family during abiotic stress and development. DNA Res. 21, 267–283 (2014).
Mudgil, Y., Shiu, S.-H., Stone, S. L., Salt, J. N. & Goring, D. R. A large complement of the predicted Arabidopsis ARM repeat proteins are members of the U-box E3 ubiquitin ligase family. Plant Physiol. 134, 59–66 (2004).
Phillips, S. M., Dubery, I. A. & van Heerden, H. Molecular characterization of an elicitor-responsive Armadillo repeat gene (GhARM) from cotton (Gossypium hirsutum). Mol. Biol. Rep. 39, 8513–8523 (2012).
Miki, T., Nishina, M. & Goshima, G. RNAi screening identifies the armadillo repeat-containing kinesins responsible for microtubule-dependent nuclear positioning in Physcomitrella patens. Plant Cell Physiol. 56, 737–749 (2015).
Dornelas, M. C., Lejeune, B., Dron, M. & Kreis, M. The ArabidopsisSHAGGY-related protein kinase (ASK) gene family: structure, organization and evolution. Gene 212, 249–257 (1998).
Dornelas, M. C., Van Lammeren, A. A. & Kreis, M. Arabidopsis thaliana SHAGGY-related protein kinases (AtSK11 and 12) function in perianth and gynoecium development. Plant J. 21, 419–429 (2000).
Supek, F., Bonjak, M., Skunca, N. & Smuc, T. REVIGO summarizes and visualizes long lists of gene ontology terms. PLoS ONE 6, e21800 (2011).
Krishnamurthy, P. et al. Transcriptomics analysis of salt stress tolerance in the roots of the mangrove Avicennia officinalis. Sci. Rep. 7, 10031 (2017).
Wang, L., Du, H., Li, T. & Wuyun, T.-N. novo transcriptome sequencing and identification of genes related to salt stress in Eucommia ulmoides Oliver. Trees 32, 151–163 (2018).
Parida, A. K. & Jha, B. Salt tolerance mechanisms in mangroves: a review. Trees 24, 199–217 (2010).
Hibino, T. et al. Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaine-accumulating mangrove Avicennia marina (Forsk.) Vierh. Plant Mol. Biol. 45, 353–363 (2001).
Redillas, M. C. et al. Accumulation of trehalose increases soluble sugar contents in rice plants conferring tolerance to drought and salt stress. Plant Biotechnol. Rep. 6, 89–96 (2012).
McCue, K. F. & Hanson, A. D. Salt-inducible betaine aldehyde dehydrogenase from sugar beet: cDNA cloning and expression. Plant Mol. Biol. 18, 1–11 (1992).
Wang, J. et al. Transcriptomic profiling of the salt-stress response in the halophyte Halogeton glomeratus. BMC Genom. 16, 169 (2015).
Gharat, S. A., Parmar, S., Tambat, S., Vasudevan, M. & Shaw, B. P. Transcriptome analysis of the response to NaCl in Suaeda maritima provides an insight into salt tolerance mechanisms in halophytes. PLoS ONE 11, e0163485 (2016).
Mishra, A. & Tanna, B. Halophytes: potential resources for salt stress tolerance genes and promoters. Front. Plant Sci. 8, 829 (2017).
Hussain, S. S., Ali, M., Ahmad, M. & Siddique, K. H. Polyamines: natural and engineered abiotic and biotic stress tolerance in plants. Biotechnol. Adv. 29, 300–311 (2011).
Gupta, K., Dey, A. & Gupta, B. Plant polyamines in abiotic stress responses. Acta Physiol. Plant. 35, 2015–2036 (2013).
Rangan, P., Subramani, R., Kumar, R., Singh, A. K. & Singh, R. Recent advances in polyamine metabolism and abiotic stress tolerance. BioMed Res. Int. 2014, 239621 (2014).
Jiménez Bremont, J. F. et al. Physiological and molecular implications of plant polyamine metabolism during biotic interactions. Front. Plant Sci. 5, 95 (2014).
Hatmi, S. et al. Drought stress tolerance in grapevine involves activation of polyamine oxidation contributing to improved immune response and low susceptibility to Botrytis cinerea. J. Exp. Bot. 66, 775–787 (2014).
Qing, D.-J. et al. Comparative profiles of gene expression in leaves and roots of maize seedlings under conditions of salt stress and the removal of salt stress. Plant Cell Physiol. 50, 889–903 (2009).
Parvin, S. et al. Modulation of polyamine levels in ginseng hairy root cultures subjected to salt stress. Russ. J. Plant Physiol. 59, 757–765 (2012).
Feyereisen, R. & Werck-Reichhart, D. Cytochromes P450: a success story. Genome Biol. 1, 1–9 (2000).
Kurotani, K.-I. et al. Elevated levels of CYP94 family gene expression alleviate the jasmonate response and enhance salt tolerance in rice. Plant Cell Physiol. 56, 779–789 (2015).
Ishikawa, T. & Shigeoka, S. Recent advances in ascorbate biosynthesis and the physiological significance of ascorbate peroxidase in photosynthesizing organisms. Biosci. Biotechnol. Biochem. 72, 1143–1154 (2008).
Guo, Y., Huang, C., Xie, Y., Song, F. & Zhou, X. A tomato glutaredoxin gene SlGRX1 regulates plant responses to oxidative, drought and salt stresses. Planta 232, 1499–1509 (2010).
Parihar, P., Singh, S., Singh, R., Singh, V. P. & Prasad, S. M. Effect of salinity stress on plants and its tolerance strategies: a review. Environ. Sci. Pollut. Res. 22, 4056–4075 (2015).
Ryu, H. & Cho, Y.-G. Plant hormones in salt stress tolerance. J. Plant Biol. 58, 147–155 (2015).
Zhao, Y. et al. A wheat allene oxide cyclase gene enhances salinity tolerance via jasmonate signaling. Plant Physiol. 164, 1068–1076 (2014).
Makhloufi, E. et al. Isolation and molecular characterization of ERF1, an ethylene response factor gene from durum wheat (Triticum turgidum L. subsp. durum), potentially involved in salt-stress responses. J. Exp. Bot. 65, 6359–6371 (2014).
Cao, W. H. et al. Expression of tobacco ethylene receptor NTHK1 alters plant responses to salt stress. Plant Cell Environ. 29, 1210–1219 (2006).
Cao, Y.-R., Chen, S.-Y. & Zhang, J.-S. Ethylene signaling regulates salt stress response: an overview. Plant Signal. Behav. 3, 761–763 (2008).
Karve, A., Xia, X. & dMoore, B. Arabidopsis hexokinase-Like1 and hexokinase1 form a critical node in mediating plant glucose and ethylene responses. Plant physiol. 158, 1965–1975 (2012).
Pujade-Renaud, V. et al. Ethylene-induced increase in glutamine synthetase activity and mRNA levels in Hevea brasiliensis latex cells. Plant Physiol. 105, 127–132 (1994).
Hossain, M., Uddin, M., Ismail, M. R. & Ashrafuzzaman, M. Responses of glutamine synthetase-glutamate synthase cycle enzymes in tomato leaves under salinity stress. Int. J. Agric. Biol. 14, 509–515 (2012).
Parida, A. K., Das, A. B. & Mohanty, P. Defense potentials to NaCl in a mangrove, Bruguiera parviflora: differential changes of isoforms of some antioxidative enzymes. J. Plant Physiol. 161, 531–542 (2004).
Abogadallah, G. M. Insights into the significance of antioxidative defense under salt stress. Plant Signal. Behav. 5, 369–374 (2010).
Hernandez, J., Jimenez, A., Mullineaux, P. & Sevilia, F. Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defences. Plant Cell Environ. 23, 853–862 (2000).
Mittova, V., Guy, M., Tal, M. & Volokita, M. Salinity up-regulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii. J. Exp. Bot. 55, 1105–1113 (2004).
Chaparzadeh, N., D’Amico, M. L., Khavari-Nejad, R.-A., Izzo, R. & Navari-Izzo, F. Antioxidative responses of Calendula officinalis under salinity conditions. Plant Physiol. Biochem. 42, 695–701 (2004).
Gao, S. et al. Effects of salt stress on growth, antioxidant enzyme and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedlings. Plant Soil Environ. 54, 374–381 (2008).
Zhang, M., Fang, Y., Ji, Y., Jiang, Z. & Wang, L. Effects of salt stress on ion content, antioxidant enzymes and protein profile in different tissues of Broussonetia papyrifera. S. Afr. J. Bot. 85, 1–9 (2013).
Murshed, R., Lopez-Lauri, F. & Sallanon, H. Effect of salt stress on tomato fruit antioxidant systems depends on fruit development stage. Physiol. Mol. Biol. Plants 20, 15–29 (2014).
AbdElgawad, H. et al. High salinity induces different oxidative stress and antioxidant responses in maize seedlings organs. Front. Plant Sci. 7, 276 (2016).
Gharsallah, C., Fakhfakh, H., Grubb, D. & Gorsane, F. Effect of salt stress on ion concentration, proline content, antioxidant enzyme activities and gene expression in tomato cultivars. AoB Plants 8, plw055 (2016).
Farhangi-Abriz, S. & Torabian, S. Antioxidant enzyme and osmotic adjustment changes in bean seedlings as affected by biochar under salt stress. Ecotoxicol. Environ. Saf. 137, 64–70 (2017).
Quan, R. et al. SCABP8/CBL10, a putative calcium sensor, interacts with the protein kinase SOS2 to protect Arabidopsis shoots from salt stress. Plant Cell 19, 1415–1431 (2007).
Li, R. et al. HbCIPK2, a novel CBL-interacting protein kinase from halophyte Hordeum brevisubulatum, confers salt and osmotic stress tolerance. Plant Cell Environ. 35, 1582–1600 (2012).
Chen, X. et al. ZmCIPK21, a maize CBL-interacting kinase, enhances salt stress tolerance in Arabidopsis thaliana. Int. J. Mol. Sci. 15, 14819–14834 (2014).
Reguera, M., Bassil, E. & Blumwald, E. Intracellular NHX-type cation/H+ antiporters in plants. Mol. Plant 7, 261–263 (2014).
Dreyer, I. & Uozumi, N. Potassium channels in plant cells. FEBS J. 278, 4293–4303 (2011).
Yazaki, K. ABC transporters involved in the transport of plant secondary metabolites. FEBS Lett. 580, 1183–1191 (2006).
Kim, D. Y., Bovet, L., Maeshima, M., Martinoia, E. & Lee, Y. The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance. Plant J. 50, 207–218 (2007).
Kim, D. Y., Jin, J. Y., Alejandro, S., Martinoia, E. & Lee, Y. Overexpression of AtABCG36 improves drought and salt stress resistance in Arabidopsis. Physiol. Plant. 139, 170–180 (2010).
Yang, T. & Poovaiah, B. Calcium/calmodulin-mediated signal network in plants. Trends Plant Sci. 8, 505–512 (2003).
Peng, Z. et al. Comprehensive analysis of differentially expressed genes and transcriptional regulation induced by salt stress in two contrasting cotton genotypes. BMC Genom. 15, 760 (2014).
Gong, D., Guo, Y., Schumaker, K. S. & Zhu, J.-K. The SOS3 family of calcium sensors and SOS2 family of protein kinases in Arabidopsis. Plant Physiol. 134, 919–926 (2004).
Capella, M., Ribone, P. A., Arce, A. L. & Chan, R. L. Arabidopsis thaliana HomeoBox 1 (At HB 1), a Homedomain-Leucine Zipper I (HD-Zip I) transcription factor, is regulated by phytochrome-interacting factor 1 to promote hypocotyl elongation. New Phytol. 207, 669–682 (2015).
Dai, X. et al. Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis. Plant Physiol. 143, 1739–1751 (2007).
Zhou, J. et al. Basic helix-loop-helix transcription factor from wild rice (OrbHLH2) improves tolerance to salt-and osmotic stress in Arabidopsis. J. Plant Physiol. 166, 1296–1306 (2009).
Guan, Q., Wu, J., Yue, X., Zhang, Y. & Zhu, J. A nuclear calcium-sensing pathway is critical for gene regulation and salt stress tolerance in Arabidopsis. PLoS Genet. 9, e1003755 (2013).
Shi, H. & Chan, Z. The cysteine2/histidine2-type transcription factor zinc finger of Arabidopsis thaliana 6-activated C-repeat-binding factor pathway is essential for melatonin-mediated freezing stress resistance in Arabidopsis. J. Pineal Res. 57, 185–191 (2014).
Allan, A. C., Hellens, R. P. & Laing, W. A. MYB transcription factors that colour our fruit. Trends Plant Sci. 13, 99–102 (2008).
Hichri, I. et al. The Solanum lycopersicum WRKY3 transcription factor SlWRKY3 is involved in salt stress tolerance in tomato. Front. Plant Sci. 8, 1343 (2017).
Song, H. et al. Global analysis of WRKY genes and their response to dehydration and salt stress in soybean. Front. Plant Sci. 7, 9 (2016).
Zhang, Y.-F., Li, G.-L., Wang, X.-F., Sun, Y.-Q. & Zhang, S.-Y. Transcriptomic profiling of taproot growth and sucrose accumulation in sugar beet (Beta vulgaris L.) at different developmental stages. PLoS ONE 12, e0175454 (2017).
Reddy, A. S., Ali, G. S., Celesnik, H. & Day, I. S. Coping with stresses: roles of calcium-and calcium/calmodulin-regulated gene expression. Plant Cell 23, 2010–2032 (2011).
Jiang, X., Leidi, E. O. & Pardo, J. M. How do vacuolar NHX exchangers function in plant salt tolerance?. Plant Signal. Behav. 5, 792–795 (2010).
Raczy, C. et al. Isaac: ultra-fast whole-genome secondary analysis on Illumina sequencing platforms. Bioinformatics 29, 2041–2043 (2013).
Grabherr, M. G. et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat. Biotechnol. 29, 644 (2011).
Pertea, G. et al. TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets. Bioinformatics 19, 651–652 (2003).
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. Basic local alignment search tool. J. Mol. Biol. 215, 403–410 (1990).
Kanehisa, M. & Goto, S. KEGG: kyoto encyclopedia of genes and genomes. Nucl. Acids Res. 28, 27–30 (2000).
Kanehisa, M., Sato, Y., Kawashima, M., Furumichi, M. & Tanabe, M. KEGG as a reference resource for gene and protein annotation. Nucl. Acids Res. 44, D457–D462 (2016).
Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25, 402–408 (2001).
Iseli, C., Jongeneel, C. V. & Bucher, P. ESTScan: a program for detecting, evaluating, and reconstructing potential coding regions in EST sequences. Proc. Int. Conf. Intell. Syst. Mol. Biol. 99, 138–148 (1999).
Conesa, A. et al. Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21, 3674–3676 (2005).
Ye, J. et al. WEGO: a web tool for plotting GO annotations. Nucl. Acids Res. 34, W293–W297 (2006).
Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nat. Methods 9, 357 (2012).
Kim, D. et al. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biol. 14, R36 (2013).
Mortazavi, A., Williams, B. A., McCue, K., Schaeffer, L. & Wold, B. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat. Methods 5, 621 (2008).
Benjamini, Y., Drai, D., Elmer, G., Kafkafi, N. & Golani, I. Controlling the false discovery rate in behavior genetics research. Behav. Brain Res. 125, 279–284 (2001).
Mao, X., Cai, T., Olyarchuk, J. G. & Wei, L. Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary. Bioinformatics 21, 3787–3793 (2005).
Dai, X., Sinharoy, S., Udvardi, M. & Zhao, P. X. PlantTFcat: an online plant transcription factor and transcriptional regulator categorization and analysis tool. BMC Bioinform. 14, 321 (2013).
Wei, W. et al. Characterization of the sesame (Sesamum indicum L.) global transcriptome using Illumina paired-end sequencing and development of EST-SSR markers. BMC Genom. 12, 451 (2011).
Jin, Y., Liu, F., Huang, W., Sun, Q. & Huang, X. Identification of reliable reference genes for qRT-PCR in the ephemeral plant Arabidopsis pumila based on full-length transcriptome data. Sci. Rep. 9, 1–11 (2019).
Acknowledgements
This work is a part of In house project of ICAR-IIVR, Varanasi. Resources of DBT-sponsored project “Genomics-assisted selection of Solanum chilense introgression lines for enhancing drought resistance in tomatoes” funded by Ministry of science and technology, Department of Biotechnology, Government of India, New Delhi were used for this work. We are also thankful to the Indian Coucil of Agricultural Research (ICAR) and Director, Indian Institute of Vegetable Research, Varanasi for providing necessary facilities. We thank Dr. B L Patil, Division of Biotechnology, ICAR-IIHR, Bangalore for critical reading of the manuscript.
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H.C.P. and B.S. conceived the research plans; H.C.P. and N.K. supervised the experiments; S.P.K. and P.M. performed most of the experiments; S.P.K., H.C.P. and P.M. carried out bioinformatics analyses; H.C.P., S.P.K. and B.S. designed the experiments; H.C.P., S.P.K. and P.M. wrote the article; H.C.P., N.K. and B.S. edited and prepared final draft of the manuscript with contributions of all the authors.
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Kashyap, S.P., Prasanna, H.C., Kumari, N. et al. Understanding salt tolerance mechanism using transcriptome profiling and de novo assembly of wild tomato Solanum chilense. Sci Rep 10, 15835 (2020). https://doi.org/10.1038/s41598-020-72474-w
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DOI: https://doi.org/10.1038/s41598-020-72474-w
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