Abstract
Excess N-fertilizer application and inappropriate fertilization methods have led to low N use efficiency (NUE) and high N leaching. A field experiment was performed in a typical lime concretion black soil area to compare N application methods: split surface broadcasting (SSB) and one-time root-zone fertilization (RZF) on grain yield, NUE, the fate of 15N urea and soil N loss during the 2015 and 2016 maize growing seasons. Each application method was tested at N rates of 135 and 180 kg N ha−1, and a control (CK) with no N fertilizer. The RZF treatment remarkably increased grain yield by 7.0% compared with SSB treatment under 180 kg N ha−1, and significantly increased N derived from fertilizer by 28.5%. The residual 15N in the 0–80 cm soil layer was 40.6–47.6% after harvest, 61.8–70.9% of which was retained in 0–20 cm. The RZF remarkably increased the 15N recovery in maize by 28.7%, while significantly decreased the potential N losses by 30.2% compared with SSB in both seasons. In conclusion, one-time RZF of urea is recommended for obtaining high yields, increasing NUE, and minimizing N losses in maize, which deserves more attention for developing and applying in the future.
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Discover the latest articles, news and stories from top researchers in related subjects.Introduction
Nitrogen (N) fertilization is one of the most important farming practices to improve grain yield of cereal crops1. High rates of N fertilizer, especially synthetic N fertilizer, are often applied to achieve high yields in China1,2; however, the increased fertilizer input has not resulted in consistently higher crop yields3,4. Instead, excess N fertilizer and poor fertilization methods have led to low N use efficiency (NUE) and high N losses1,5, resulting in a great number of environmental problems, such as water pollution, atmospheric contamination, and soil quality degradation6,7. Therefore, efficient N fertilizer management is vital to increase crop yields, improve soil fertility and minimize environmental risk8,9,10. Effective N management can improve NUE, and depends largely on the combination of appropriate sources, application rates, timing, and placement of N fertilizer application11,12,13. Numerous studies have been conducted to improve crop yield and NUE, and reduce N losses by adjusting the N application rate5,14,15. Many researchers have reported that under the same N application rate, splitting the number of N applications according to the plant’s N needs can reduce N losses and increase yield and NUE14,16. Moreover, using enhanced N fertilizers, such as urease inhibitors and controlled release fertilizer, have been shown to improve NUE and yields in rice, wheat and maize crops17,18. However, more labor or higher prices have limited the expansion of this alternative techniques19,20,21.
Increasing the N split application numbers according to the plant’s N needs was suggested for increasing NUE and reducing N losses16,19. Wang et al.5 reported that the N recovery efficiency for 2-split and 3-split application of urea was significantly higher than that for one-time mixed basal dressing, while the potential N loss was decreased remarkably. However, recently, Yao et al.21 found that point deep-placement at one time as a basal fertilizer significantly increased N recovery efficiency (NRE) by 55%, while decreased ammonia (NH3) volatilization by 91% compared to surface split broadcasting treatments. Grain yield and agronomic efficiency of fertilizer deep placement were superior to conventional farmer’s split method19. Therefore, split fertilization can be replaced by one-time basal fertilization only if the fertilization method was improved effectively. It has been widely recognized that one-time urea deep placement (UDP) can increase crop yields and reduce N losses22,23,24,25,26. We recently reported that root-zone fertilization (RZF) was effective in reducing N losses in the rice paddy fields27 and in the wheat–soil system4. RZF is a much more exact deep placement (DP) of fertilization according to the specific crop. For summer maize, we found that N applied all at one time as a basal fertilizer into a hole 5 cm away from the seed and 12 cm under the soil surface was considered to be a suitable RZF28. However, few studies have assessed the fate of N fertilizer under different application methods or the potential losses of N under RZF in a summer-maize dryland, specifically in the Huang-Huai-Hai Plain of China.
Huang-Huai-Hai Plain is one of the most important agricultural regions in China. The dominant cropping system is a rotation of summer-maize/winter-wheat29,30. One of the most important soil types in the region is the lime concretion black soil, also called Shajiang black soil, which plays an important role in food production31. The lime concretion black soil is found mainly in Huaibei Plain (located in the southern of the Huang-Huai-Hai Plain), which is characterized by heavy texture, low soil physical quality and soil fertility31. Lime concretion black soil was exhibited lower NUE in wheat than that of Chao soil, and lower N retention32, but showed a faster release of urea than that in fluvo-aquic soil33. However, limited information is available on the effect of urea application method on NUE in lime concretion black soil. The arable land in the Huang-Huai-Hai Plain is characterized by intense farming, with high rates of N fertilizer inputs and high crop yields29. In this region, the annual N fertilizer application for all crops amounts to 500 kg N ha−1 34, and the N rate for maize has been reported to be 270 kg N ha−1, with a low NUE of about 28.5%35. However, in 2015, the Chinese Ministry of Agriculture issued Zero Increase Action Plan to curb the increase in fertilizer usage to zero by 202036, which aims to increase crop yields without increasing fertilizer use, in an effort to reduce negative human impacts and environmental costs. To achieve this goal, it is imperative to improve NUE and reduce N losses, without further increase of N fertilizer use even decreases N application by 20%.
In maize production, N fertilizers are typically split into two applications in early June and late July, using the traditional surface broadcasting. The current practice of SSB N application is labor intensive and causes large amount of N to leach into the environment, yet it does not substantially increase maize yields10,37. Therefore, a better N management strategy based on reducing N losses and substantially increasing yields is needed. Our recent study showed that RZF has great promise for accomplishing both of these goals in the rice paddy fields27,28,38, while not effective in the winter wheat-soil system4. Although both the maize–soil system and wheat-soil system are dryland, the growth period of maize is about only 4 months, which is obviously shorter than that of wheat (nearly 8 months). Therefore, the fate of fertilizer N under the application of RZF in the dryland system, especially in the maize–soil system, should be played more attention. Here, we used 15N-labeled urea fertilizer to track the movement of N under two fertilization application methods, two-application SSB and one-time RZF, in a maize cropping system. A field experiment was conducted during two consecutive maize cropping seasons (2015 and 2016) in the Huang-Huai-Hai Plain of China.
Results
Maize yields
N rate and application method significantly affected grain number per ear, grain yield and biomass of maize (Fig. 1). RZF of urea greatly increased grain number per ear of maize compared to surface broadcasting treatments. However, the 1000-grain weight of maize was not significantly affected by N application method in both 2015 and 2016. Grain yield of maize was significantly higher in the fertilizer treatments (10.9–12.4 t ha−1) than in the control (8.7 t ha−1) in both seasons. The grain yield was 6.9% and 7.0% higher in RZF180 compared with SSB180 in 2015 and 2016, respectively. However, there was no significant difference between SSB and RZF at low doses of N (135 kg ha−1) in both 2015 and 2016. The grain yield of RZF180 was significantly higher than that of RZF135, while they were not significantly different between SSB180 and SSB135. The biomass in RZF180 (19.3 t ha−1) was significantly higher than in SSB180 (18.6 t ha−1) in 2016.
Effects of method of application of N and its dose on grain number, 1000-grain weight, grain yield and biomass of maize. CK: N application 0 kg ha−1, SSB135: two-split surface broadcasting with 135 kg N ha−1; SSB180: two-split surface broadcasting with 180 kg N ha−1; RZF135: one-time root-zone fertilization with 135 kg N ha−1; RZF180: one-time root-zone fertilization with 180 kg N ha−1. Columns labelled with different letters between treatments represent significant differences (P < 0.05).
N concentration and N uptake by maize
The N concentration in both grain and straw were significantly affected by the applied doses of N, but not significantly affected by application method during two consecutive growing seasons (Table 1). In 2015, the concentrations of N in grains were significantly higher in RZF135 (13.4 g kg−1) and RZF180 (13.9 g kg−1) than that in SSB135 (12.8 g kg−1) and SSB180 (13.1 g kg−1), whereas there were no differences in 2016. There was no substantial difference in N concentration in straw between any of the fertilizer treatments (Table 1). N uptake by grain and straw and total N uptake by plant were all significantly affected by N dose. In both seasons, the N uptake in maize plants increased with increasing N application rate. Overall, the total N uptake by the whole plant in RZF was higher than that in SSB: by 5.8% and 4.5% in 2015 and 2016, respectively (Table 1).
Nitrogen-use efficiency
The N apparent recovery efficiency (NARE), N agronomy efficiency (NAE) and N partial factor productivity (NPFP) of maize over two consecutive growing seasons are shown in Table 2. The application dose of N had a significant effect on NAE and NPFP, but did not affect NARE. The NAE and NPFP were significantly higher at 135 kg N ha−1 treatments (23.2 and 86.7 kg kg−1, respectively) than that of 180 kg N ha−1 treatments (19.0 and 66.5 kg kg−1). The N application method had a significant effect on NARE, but did not affect NPFP over two consecutive growing seasons; and also affected the NAE in 2015, but did not significantly affect the NAE in 2016. Compared with the SSB, RZF significantly increased NARE by 14.1% and 19.4% at 135 and 180 kg N ha−1, respectively.
Sources of plant N uptake and 15N distribution in maize
N derived from fertilizer (Ndff) was significantly affected by N application method, N dose, and interaction between N application method and dose in 2015 and 2016, whereas neither N application method nor N dose significantly affected the N derived from soil (Ndfs) (Table 3). In both seasons, the Ndff at 180 kg N ha−1 treatments was 21.3% higher than that of 135 kg N ha−1 treatments. In RZF treatments, the Ndff increased from 17.5% to 22.9% on average when the N application rate increased form 135 to 180 kg ha−1. Moreover, the Ndff was 28.5% higher on average in RZF compared with SSB in 2015 and 2016.
Neither N application method nor N dose significantly affected the distribution of 15N among different organs of maize, except that 15N uptake in roots was significantly higher in the high N application method in 2015 (Fig. 2). The distribution of 15N among different organs was as follows: grain > straw > root. In 2015 and 2016, 56.1–58.1% and 54.5–58.0%, respectively, of total 15N uptake by the plant was partitioned to grain. Overall, the distribution of 15N in grain, straw and root did not significantly changed by N application method or N dose.
Distribution of 15N-labeled urea in maize organs as a factor of application dose (135 or 180 kg N ha−1) and application method (root zone fertilization, RZF, or split surface broadcast, SSB) in two consecutive growing seasons (2015–2016). SSB135: two-split surface broadcasting with 135 kg N ha−1; SSB180: two-split surface broadcasting with 180 kg N ha−1; RZF135: one-time root-zone fertilization with 135 kg N ha−1; RZF180: one-time root-zone fertilization with 180 kg N ha−1.
Distribution of residual 15N-labeled urea in soil
The pattern of residual 15N distribution in the soil among treatments was not different for either growing seasons (Fig. 3). The total residual 15N in the 0–80 cm soil profile layer after maize harvest was 54.8–64.3 kg ha−1 in the two growing season under 135 kg N ha−1 treatments, accounting for 40.6–47.6% of 15N, and 73.8–81.7 kg ha−1 under 180 kg N ha−1 treatments, accounting for 41.0–45.4% of 15N, with about two-thirds (67.3%) of the residual 15N remaining in the 0–20 cm soil layer. The total residual 15N in the 0–80 cm soil profile layer was significantly affected by neither N application method nor N dose. However, the distribution of residual 15N in soil profile layer was significantly affected by the N application method, especially in 0–20 cm soil layer. The percentage of residual 15N in 0–20 cm soil layer was slightly higher in SSB (69.1%) than that in RZF (65.7%).
The distribution of residual 15N at the end of each harvest season as a factor of application method and rate of N. SSB135: two-split surface broadcasting with 135 kg N ha−1; SSB180: two-split surface broadcasting with 180 kg N ha−1; RZF135: one-time root-zone fertilization with 135 kg N ha−1; RZF180: one-time root-zone fertilization with 180 kg N ha−1.
Fate of 15N-labeledled urea in maize–soil system
The N recovery and potential losses were significantly affected by N application method, whereas the residual was affected by neither N application method nor N dose over two consecutive growing seasons (Table 4). In RZF, the recovery of 15N-labeled urea in maize was 28.2–38.2% at 135 kg N ha−1 treatments and 28.0–42.0% at 180 kg N ha−1 treatments. Therefore, the N dose did not significantly affected the fate of 15N-labeled urea in maize growing seasons. On average, the recovery of N was 28.7% higher in RZF than that in SSB over two years. The potential N losses were 17.1–26.2% in RZF and 27.2–35.7% in SSB. Overall, the 2-year average potential N losses were 30.2% lower in RZF than that in SSB.
Discussion
In the present study, the grain yield was 7.0% higher in RZF180 compared with SSB180 in both seasons. Chen et al.14 reported a mean maize yield of about 13.0 t ha−1 at N rate of 237 ± 70 kg ha−1 on 66 on-farm experimental plots under an integrated soil–crop system management. Moreover, the maize grain yield reached 14.8 t ha−1 at 250 kg N ha−1 under a high-yield maize system in northern China39. However, the maize yield in this study was significant higher than that of another study in Huang-Huai-Hai Plain of China, which reported that the maize grain yield was only 9.0 t ha−1 under the N application rate of 225 kg ha−1 30. Yang et al.40 also reported that grain yield of summer maize was 10.5 t ha−1 at 180 kg N ha−1 in North China Plain of China. Therefore, both the RZF180 and SSB180 treatments in the present study could be classified as “high” yields of summer maize. However, RZF of urea lead to higher grain number per ear and aboveground biomass which have a positive relationship with yield compared with surface broadcasting. Therefore, one-time RZF of urea achieved higher grain yield and biomass than 2-split surface broadcasting for summer maize system in this study.
Previous studies have reported that deep placement of N significantly increases N uptake by crop plants27,41,42. Rees et al.41 found that N uptake by maize under N deep placement increased by 7.9–32.5% compared with surface broadcasting. We also found that one-time urea RZF can promote N uptake by rice and thus increase grain yield, compared with three-split surface broadcasting treatments27. These results agree with the present study; we found that N placement significantly affected the N uptake in grain and the whole plant. Although there was interannual variability due to differences in weather conditions (mainly in rainfall), the N uptake by maize was substantially higher in RZF than SSB treatments in both 2015 and 2016 (Table 1). Compared with surface broadcasting, deep placement of urea can provide more N for the deep regions of the rhizosphere, thus improving the growth of roots and N uptake by plants43. Moreover, deep placement of N fertilizer prolonged N availability up to flowering19, and increased the number of tillers and panicles in rice27,44.
Fertilizer placement had a significant effect on N uptake from fertilizer. The Ndff was 18.4% and 39.2% higher in RZF than SSB for 135 and 180 kg N ha−1 treatments, respectively, over two consecutive growing seasons (Table 3). These results are in agreement with previous work that found that point placement significantly increases the Ndff (%) by 30.4% compared with surface application, in summer maize41. Yao et al.21 also reported that N uptake by rice derived from fertilizer was 62% higher in one-time UDP treatment than that in surface broadcasting. The higher Ndff in RZF may be attributed to point deep placement significantly increasing the nutrient content in the root zone27,43 and decreasing N losses through runoff and NH3 volatilization, compared with surface applications43,45. In addition, the Ndff (%) was higher in the higher N application rate treatments for both RZF and SSB. The Ndff (%) under 180 kg N ha−1 treatments increased by 30.2% and 12.3% compared with 135 kg N ha−1 treatments in RZF and SSB, respectively (Table 3). This is consistent with previous studies by Yang et al.40, who found that the Ndff (%) in the total maize plant increased from 23.7% to 43.6% with increasing N application rate from 90 to 135 kg N ha−1. Chen et al.46 also reported that the Ndff (%) of winter wheat increased from 34.6% to 41.8%, when the N dose increased from 150 to 240 kg ha−1. In addition, the suitable N fertilization rate for the summer maize in Huang-Huai-Hai Plain47 and the lime concretion black soil region48 were 113–180 and 144–225 kg ha−1, respectively. Overall, these results suggest that the rate of 135 kg N ha−1 is not sufficient for maize plants, while the application rate of 180 kg N ha−1 may be generally suitable for obtaining high yields and maintaining low potential N losses for maize grown in lime concretion black soils of the Huang-Huai-Hai Plain. The present study suggests that one-time RZF can further reduce the amount of N fertilizer, which deserves more attention and further research in the future.
In the present study, the NUE (NARE and N recovery) of RZF were significantly higher than that of SSB (Tables 2 and 4). High NUE obtained with deep placement agrees with the findings from other studies41,49,50. For example, Rees et al.41 reported that the NRE of summer maize under deep placement increased from 18% to 25% compared with surface application. Similarly, Yao et al.21 found that one-time UDP significantly increased NARE by 52.6% during three consecutive rice cropping seasons compared with 3-split surface broadcasting. Therefore, this study suggests that N point deep placement in the root-zone can increase N recovery in maize and improve NUE.
The potential N losses in all treatments were 17.2–35.7% (Table 4), consistent with previous reports. Yang et al.40 found 14–33% N losses in summer maize in North China Plain. Ju et al.51 reported that total N fertilizer losses for summer maize were 21.1–51.3% in the North China Plain, with a higher potential N loss at the conventional application rate (360 kg N ha−1). However, the potential N losses of this study were higher than that of maize in semiarid farmland reported by Wang et al.5 (averaged 15.4% among all treatments). The lower potential N losses reported by Wang et al.5 may be attributed to the plastic mulching practice, which could decrease N leaching losses and result in higher levels of 15N-labeled remaining in the 0–170 cm soil profile5,43. Compared with surface broadcasting, root-zone fertilization greatly reduced total potential N losses. In both seasons, the potential N losses were 17.1–26.2% in RZF, which was 30.2% lower than that in SSB (27.2–35.7%) (Table 4). This is consistent with Cai et al.45, which reported that total N loss for maize in deep placement decreased from 54.5% to 18.5% compared with surface broadcasting. Similarly, the 15N loss was 38% lower for one-time deep placement of urea compared with three-split surface broadcasting21. Deep placement of urea enormously reduced both NH3 volatilization and denitrification losses compared with split surface broadcasting43,45, probably as a result of better preservation and concentration of N fertilizer beneath plant roots and the reduction of soil microbes competing with plants for the point deep-placed N21,27. In contrast, when urea was broadcast onto the soil surface, it was easily lost through NH3 volatilization and runoff21. Therefore, as a much more exact deep placement of fertilization, the RZF of urea could substantially reduce ammonia volatilization, denitrification loss and runoff for maize systems in lime concretion black soil of the Huang-Huai-Hai Plain. In general, RZF is a fertilizer-saving and N-loss reducing fertilization method, but it is worth developing and applying the special root-zone fertilization machinery.
RZF of urea did not affect the total residual 15N in the 0–80 cm soil profile layer. On average, the residual 15N-labeled fertilizer in the 0–80 cm soil layer was 59.6 and 78.0 kg ha−1 under 135 and 180 kg N ha−1 treatments, respectively, accounting for 44% and 43% of the total 15N application (Fig. 3 and Table 4). This result is supported by that obtained for maize in North China Plain by Yang et al.40, who found that 45–60% of the applied N remained in the 0–150 cm soil layer at the first maize harvest. Wang et al.5 also found that the residual 15N in the 0–200 cm soil layer was about 48.3–51.3% at harvest in semiarid plastic mulched maize cropping system. Moreover, we found that two-thirds (61.8–70.9%) of the residual 15N was retained in the 0–20 cm layer (Fig. 3). Yang et al.40 and Wang et al.5 reported that approximately half of the residual N remained in the 0–20 cm layer at the summer-maize harvest. However, in our previous study, 76.8–87.0% of total residual N remained in the 0–20 cm soil layer for winter wheat in south-eastern China46. The difference may be due to differences in soil properties; in Yang et al.40, the soil in the test site was a sandy loam, which may increase the risk of N leaching compared with the loamy soil in the Chen et al.46 experiment. Moreover, the point deep placement can reduce nitrate and total N leaching losses21,52, thus more residual 15N was retained in the topsoil (0–20 cm). Therefore, our results suggest that RZF may be an effective approach to further reduce N loss through leaching for summer maize, especially with high rainfall and course-textured or sandy soils.
In conclusion, one-time RZF of urea (180 kg N ha−1) achieved 7.0% higher grain yield than SSB treatment. RZF significantly increased the Ndff in maize plants by 28.5% compared with SSB. The residual 15N in the 0–80 cm soil layer ranged from 40.6% to 47.6% at maize harvest, approximately two-thirds (61.8–70.9%) of which was retained in the 0–20 cm soil layer. RZF significantly enhanced NARE and N recovery in maize compared with SSB in both seasons, while significantly decreased the potential N losses by 30.2%. Overall, our study suggests that one-time RZF of urea is a superior N application method for obtaining high-yield levels, increasing NUE, and minimizing soil N losses in the maize cropping system.
Materials and Methods
Experimental site
The experiment was performed in a farmer’s field adjacent to the research station of the Anhui Academy of Agricultural Sciences (AAAS) in Taihe County (33°15′N, 115°36′E), Anhui province, China. The soil of the experimental site is classified as a typical lime concretion black soil. Before the experiment in June 2015, the physicochemical properties of the topsoil (0–20 cm) are pH 8.0 (H2O), 18.5 g kg−1 organic matter, 1.33 g kg−1 total N, 90.6 mg kg−1 available N, 22.6 mg kg−1 available P, and 250.4 available K, 1.30 g cm−3 soil bulk density. The study site is described as a warm temperate and semi-humid monsoon climate, with a mean temperature of 14.9 °C and mean annual precipitation of 900 mm (from 1953 to 2009). The mean monthly rainfall and air temperature during the experimental period in 2015 and 2016 is shown in Fig. 4.
Experiment design and agricultural management practices
As the common in this area, summer-maize/winter-wheat rotation was used in this study. The field plot experiment was conducted for the two consecutive maize cropping seasons (2015–2016). Five treatments were assigned to the field plots: (1) a control with no N fertilizer (CK); (2) two-split surface broadcasting (SSB) with 135 kg N ha−1 (SSB135); (3) two-split surface broadcasting with 180 kg N ha−1 (SSB180); (4) one-time RZF with 135 kg N ha−1 (RZF135); and (5) one-time RZF with 180 kg N ha−1 (RZF180). The rates of N were selected according to the effect of different N rates (0, 90, 135, 180, 225, 270, and 360 kg N ha−1) on maize yield in our preliminary study (data no showed), and the previous studies carried out in Huang-Huai-Hai Plain47 and the lime concretion black soil region48, which recommended that the suitable N fertilization rate were 113–180 and 144–225 kg ha−1, respectively. The rates and timing of fertilizer application for the five treatments are shown in Table 5.
The five treatments were arranged in a randomized complete block design with three replicate plots of each treatment for a total of 15 plots each year. Plots were 2.4 m (4 rows) × 2.8 m length (40 plants for each plot) in 2015 and 3.0 m (5 rows) × 5.6 m length (100 plants for each plot) in 2016. To monitor the fate of 15N-labeled fertilizer, three plants were used within each plot, which were in the center of each main plot. Each main plot was separated by earthen banks (30 cm-wide and 50 cm-high) to prevent lateral water and nutrient inference, and each 15N fertilized plant was bordered by polyvinyl chloride (PVC) frame (50 cm-high, 28 cm-wide and 60 cm-long; open at the top and bottom) inserted into the soil to a depth of 45 cm. The maize cultivar was Zea mays L., cv ‘Longping 206’, a local prevailing cultivar, and the maize seeds were sowed at a spacing of 60 cm × 28 cm (60000 plant ha−1) in all treatments. The 15N-labeled urea (46% N content, and 10.15% of 15N abundance ratio) used in this experiment was provided by the Shanghai Research Institute of Chemical Industry. The non-labeled N fertilizer was urea (46% N). The phosphorus (P) fertilizer (135 kg P2O5 ha−1, superphosphate) and potassium (K) fertilizer (180 kg K2O ha−1, potassium chloride) were applied to were broadcast as basal fertilizers in all treatments at sowing. In SSB, 50% N fertilizer was broadcast by hand at sowing, and 50% at the tasseling stage. In the RZF treatment, the N fertilizer was point deep-placed all at one time as a basal fertilizer (4.89 and 6.52 g urea plant−1 for RZF135 and RZF180, respectively) into a hole 5 cm away from the seed and 12 cm under the soil surface, and the fertilization point was marked immediately.
Irrigation, pesticide and herbicide applications were the same for all treatments. The irrigation was applied twice (at the bell and grain-filling stages) by sprinklers during the maize growing season. For each time, sprinkler irrigation was applied until the soil surface was moist. Maize was sown by hand on June 17, 2015 and June 15, 2016, and harvested on September 27, 2015 and September 25, 2016.
Plant and soil sampling and analysis
At maturity, grain yield and biomass were determined by harvesting all plants in each plot. Grain number per plant and 1000-grain weight was also measured for each plot. The 15N labelled plants in each plot were harvested close to the ground, and divided into grain and straw. And a total of 45 plant roots of the 15N labelled plants from the 0–60 cm soil layer were collected and washed. The fresh grain, straw, and root samples were dried at 70 °C before being ground to powder and passed through a 0.15-mm screen for N content and 15N analysis.
Soils were sampled in a 20-cm radius around each plant to a depth of 80 cm at 20-cm interval. Three plants were randomly selected for soil sample. Therefore, 12 soil samples were taken for each treatment, and a total of 48 soil samples were obtained. The soil samples were air-dried, then ground through 0.15-mm sieve. Soil bulk density was determined after harvesting the wheat using the cutting ring method53. Grain, straw, root, and soil samples were analyzed for total N and 15N abundance using an elemental analyzer (Costech ECS4010, Costech Analytical Technologies Inc., Valencia, USA) coupled to an isotope ratio mass spectrometer (Delta V Advantage, Thermo Fisher Scientific Inc., USA).
Calculation methods
All 15N was expressed as the atom percent excess corrected for background abundance (i.e. 0.366%). The percentage of N derived from fertilizer (Ndff) was calculated according to the following equation46,54:
where A is the 15N natural abundance, B is the 15N atom percent excess in the plant or soil, and C is the 15N atom percent excess in the fertilizer N.
The amounts of plant N derived from fertilizer (Ndff) and from soil (Ndfs), and the soil residual N were calculated in López-Bellido et al.54.
The N fertilizer accumulation and recovery by maize were calculated according to López-Bellido et al.54 and Wang et al.5 using the following equations:
The NUE indexes, namely NARE, NAE and NPFP were calculated by following the method described by López-Bellido et al.54 and Liu et al.43.
Statistical analysis
Statistical analysis was conducted using SPSS 19.0 for analysis of variance (ANOVA). Two-way ANOVA was conducted to assess the effects of N fertilizer placement and N rate on maize yield, N uptake and fate of 15N-labeled urea. Treatments were compared by the least significance difference at α < 0.05.
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Acknowledgements
The study was financially supported by the National Basic Research Program of China (2013CB127401), the Natural Science Foundation of Anhui Province (1708085MC54), and the Discipline Construction Project of Anhui Academy of Agricultural Sciences (17A0921).
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C.J., H.W., D.L., J.S. and S.W. designed and performed the study. H.W., J.Z. and C.Z. guided the experimental processes. C.J., D.L. and H.W. analyzed the data. C.J. and H.W. wrote the manuscript and drew all figures. C.J., H.W., D.L. and Z.G. revised the manuscript.
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Jiang, C., Lu, D., Zu, C. et al. One-time root-zone N fertilization increases maize yield, NUE and reduces soil N losses in lime concretion black soil. Sci Rep 8, 10258 (2018). https://doi.org/10.1038/s41598-018-28642-0
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DOI: https://doi.org/10.1038/s41598-018-28642-0
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