Abstract
Gene expression levels exhibit stochastic variations among genetically identical organisms under the same environmental conditions. In many recent transcriptome analyses based on RNA sequencing (RNA-seq), variations in gene expression levels among replicates were assumed to follow a negative binomial distribution, although the physiological basis of this assumption remains unclear. In this study, RNA-seq data were obtained from Arabidopsis thaliana under eight conditions (21–27 replicates), and the characteristics of gene-dependent empirical probability density function (ePDF) profiles of gene expression levels were analyzed. For A. thaliana and Saccharomyces cerevisiae, various types of ePDF of gene expression levels were obtained that were classified as Gaussian, power law-like containing a long tail, or intermediate. These ePDF profiles were well fitted with a Gauss-power mixing distribution function derived from a simple model of a stochastic transcriptional network containing a feedback loop. The fitting function suggested that gene expression levels with long-tailed ePDFs would be strongly influenced by feedback regulation. Furthermore, the features of gene expression levels are correlated with their functions, with the levels of essential genes tending to follow a Gaussian-like ePDF while those of genes encoding nucleic acid-binding proteins and transcription factors exhibit long-tailed ePDF.
Similar content being viewed by others
Introduction
Stochastic variations in gene expression—known as gene expression noise or phenotype fluctuation—have been observed among individuals in a genetically identical population under the same environmental conditions1,2,3,4,5,6,7,8,9,10,11. Such variations are thought to be important for maintaining the pluripotency of embryonic stem cells, cell fate decisions, and cellular differentiation in multicellular organisms12,13,14,15. In rice, genes related to stress responses exhibited larger variations than those involved in other processes16. Furthermore, recent studies in Escherichia coli, the budding yeast Saccharomyces cerevisiae, and Arabidopsis thaliana have reported that the magnitude of gene expression noise is positively correlated with plasticity—i.e., the variation in expression levels due to mutation or environmental change17,18,19,20,21,22,23,24,25,26,27.
Recent gene expression analyses with sufficiently large replicates have shown that in organisms as diverse as E. coli and mammals, fluctuations in protein expression level for a given gene follow a log-normal distribution2,6,7,17. The closely related Frechet distribution was also proposed to describe variations in gene expression levels in E. coli and S. cerevisiae28. On the other hand, mathematical modeling of protein expression in E. coli suggested that such variations were more closely approximated by a gamma distribution, which is often considered as log-normal8,29.
In many recent high-throughput RNA sequencing (RNA-seq) studies30,31, variations in gene expression (transcription) levels among replicates were assumed to follow a log-normal distribution32,33 or a negative binomial (NB) distribution34,35,36. An analysis of RNA-seq data from a two-condition, 48-replicate experiment using S. cerevisiae revealed that variations in expression levels for each gene conformed to both log-normal and NB distributions36. Beta-binomial and Benford distributions have been proposed for fitting gene expression data obtained by RNA-seq37,38. However, the physiological basis of these distributions and the significance of associated parameters remain unclear. Furthermore, it is not known whether such model distributions are applicable to any genes in any organism, especially multicellular organisms.
Gene expression noise in plants has been investigated in rice and Arabidopsis16,27,39. However, recent studies were based on transcriptome data from experiments with few replicates40,41,42,43,44,45, which limited the inferences that could be made regarding the distribution characteristics of gene expression levels. In the present study, we analyzed RNA-seq data for A. thaliana under eight conditions (21–27 replicates) to observe the empirical probability distribution profiles of gene expression levels among individuals in a homogeneous population. We fitted the distribution profiles with a novel function that we termed the Gauss-power (G-P) mixing distribution function, which was derived from a simple stochastic transcriptional network model containing a feedback loop. Moreover, the features of each probability distribution function in gene expression level are expected to be correlated with the strength of feedback regulation for each gene, its average expression level, and its function.
Results
Analysis of Arabidopsis RNA-seq data
RNA-seq data from 7- and 22-day-old Arabidopsis shoots cultured under a 12:12-h light/dark cycle were obtained 1, 7, 13, and 19 h after the lights were turned on. There were 21 to 27 replicates for each condition. In total, 189 individual plants were analyzed by RNA-seq. We obtained 8.4 million reads on average; one sample with fewer than 1 million reads mapped to genes was omitted from subsequent analyses. The expression level was quantified according to a previously described pipeline46 (Table S1). For each condition, we examined the distribution profiles of expression levels of ~10,000 genes (Table 1) whose expression levels could be regarded as stationary (see Materials and Methods).
Cluster analysis of empirical cumulative distribution function (eCDF) profiles of genes
eCDF profiles were obtained for each gene under each condition (harvest time and age of plant) by arranging its expression levels in ascending order (Fig. 1). For most genes, the eCDFs showed typical profiles but were very noisy. We then performed a cluster analysis to estimate an ideal curve of eCDF profiles from which noise has been removed for each gene (see Materials and Methods).
For each condition, 12–15 clusters were obtained from normalized eCDF profiles (Tables S2 and S3), representing the relationship between standardized expression levels for mean = 0 and standard deviation = 1 and normalized eCDF from 0 to 1. The average values of standardized expression levels of genes belonging to the same cluster were expected to reflect the essential features of their eCDF profiles. Thus, these average values were considered in order to estimate and analyze eCDF profiles of each gene. Since gene expression levels are non-negative, we analyzed normalized eCDFs that were shifted such that the minimum value on the horizontal axis was assumed to be 0 (the value on the horizontal axis represents normalized expression level) (upper and lower left in Fig. 2).
Examples of eCDF and ePDF for indicated clusters. Representative profiles of eCDF (left) and ePDF (right) as a function of normalized expression levels for three clusters. Red and blue represent curves fitted with the G-P and NB distribution functions, respectively. Least square error was estimated for the fitting curves.
Inferences on probability density distribution profiles of gene expression levels
The derivative of eCDF yielded the profiles of empirical probability density function of normalized expression levels (ePDF) for each cluster (Figs 2 and S1–S8). The derivative of this function was estimated by differential approximation (see Supplementary Information S1). ePDF profiles obtained from the clusters showed variable shape, including Gaussian and power law-like distributions.
G-P mixing distribution function
The ePDF profiles of gene expression levels were systematically classified based on the following mathematical model. A novel probability density function, which we refer to as Gauss-power mixing function (G-P function), is described by equation 1.
This equation is a fitting function of the probability density function of expression level x of the gene of interest X; the parameter A is a normalized coefficient; and f, g, and K are constants whose physiological significance is described below.
In general, the expression levels of genes are increased by activation and decreased by inhibition of upstream genes, and influence the expression levels of their downstream targets in a gene regulatory network. They are also positively or negatively regulated by the expression levels of their downstream targets either directly or indirectly because the gene regulatory network includes many positive and negative feedback loops (Fig. 3). Thus, the expression level of each gene may regulate itself through such feedback loops. Furthermore, gene expression levels always exhibit and are influenced by stochastic noise to a degree that is correlated with the levels themselves.
Thus, a simplified model of the temporal change in the expression level x of gene X influenced by upstream genes and feedback regulation (Fig. 3) is given by equation 2:
where R(t) and \(\eta \)(t) are assumed to be Gaussian white noise with \(\langle R(t)\rangle =\langle \eta (t)\rangle =0\), \(\langle R(t)R(t^{\prime} )\rangle =2D\delta (t-t^{\prime} )\), and \(\eta (t)\eta (t^{\prime} )=2\delta (t-t^{\prime} )\); the parameters G, K, F, and C are ~ [average activation rate of X by upstream genes], ~ [average expression level of X required to induce maximum expression of downstream genes], ~ [magnitude of feedback effects], and [degradation rate of X], respectively. If D/C→0 is assumed, P(x) gives the steady-state probability distribution of x where g = G/C and f = F/C (see Supplementary Information S2).
It is worth noting that the parameter K can be eliminated from Eq. (2) by appropriate variable conversion that decreases the number of parameters in the model. From this model, a G-P function with two parameters only can be derived. However, in such a two-parameter-model, a specific scale of x is given in the process of elimination of K. Thus, generally Eq. (1) appears to be appropriate for the fitting and analysis of ePDF profiles. Moreover, K and F (or F/K) characterize the feedback effect, similar to the Michaelis constant and maximum reaction velocity, respectively, of various biochemical reactions. We, therefore, included these parameters in the present argument.
Fitting of ePDF profiles
ePDF profiles of each cluster under each condition were fitted with the G-P function and (generalized) NB function according to equation 3:
where Γ(r) is the gamma function; s, k, and Q are fitting parameters; and B is a normalized coefficient. Note that the parameter s—which is usually equal to 1—contributes to the generalization for various scales of x.
The characteristics of ePDF profiles for some clusters can be extracted from plots with a linear scale axis; however, it is more difficult to extract those of profiles with much larger maximum values and that exhibit power law-like profiles, for which log-log plots seem more suitable when the maximum ePDF value is greater than 3. In order to extract their detailed characteristics, ePDF profiles were fitted using a typical least squares method for maximum ePDF values <3; ePDF fitting parameters were chosen so as to minimize the sum of squared errors between log[ePDF] and log[fitting functions] when the maximum ePDF value was >3.
The results of fitting by the G-P and NB functions suggest that the G-P function has a least square error that tends to be smaller than that of the NB function for ePDF profiles of most clusters (Fig. 2 and Table S3), whereas the G-P function has a smaller LSE than the NB function for ~82% of ePDF profiles of clusters (86/105 clusters). It should be noted that both G-P and NB functions include the same number of parameters. Thus, the former provides a function that better fits ePDF profiles than the latter. Therefore, in subsequent analyses the ePDF profiles were classified according to a G-P function.
Classification of ePDF profiles
When ePDF profiles of each cluster were fitted with the G-P function, they were classified by three clearly divided groups with different log(K/g), K = 0 or log(K/g) <−1.3, −0.6 <log(K/g) <0.1, and log(K/g) >0.4 (Fig. 4 and Table S3). Here, log(f/g) showed a strong positive correlation with log(K/g) in second and third groups of log(K/g) (Fig. 4). From Eq. (2), the power-law distribution of x was obtained over a wide range of log x in the case of a much larger K/g (and f/g), while a Gaussian distribution was obtained in the case of a much smaller K (see Supplementary Information S2). Thus, a G-P function with K ≫ g was closer to a power law distribution. Based on these facts, ePDF profiles could be classified as one of three types: Gaussian (K ≪ g), power law-like (K ≫ g), or intermediate (K ≈ g) (Table S3). When the influence of feedback effects is large relative to other mechanisms regulating gene expression, gene expression levels exhibit a long-tailed power law-like distribution.
Relationships among fitting parameters of the G-P function from each ePDF profile of each cluster. Scatterplot of f/g and K/g of the G-P function that fits each ePDF profile of each cluster. Overall image (right) and local image near the origin (left). (f/g, K/g) clearly distinguish three groups—i.e., log(K/g), K = 0 or log(K/g) <−1.3 (blue), −0.6 <log(K/g) <0.1 (black), and log(K/g) >0.4 (red). For convenience, when K = 0, log (K/g) = log (10−4/g) is plotted.
Even for the same gene, ePDF profiles varied depending on plant age and harvest time (Table S2). In particular, genes exhibiting an intermediate ePDF profile at one time point tended to exhibit other profiles at other time points (Table S2). The ratio of occurrence of Gaussian, intermediate, and power law-like distributions at four time points in younger plants (7 days old) was ~30:26:44, while that of older plants (22 days old) was ~45:29:26. High average expression levels were more frequently associated with a Gaussian as compared to a power law-like distribution; average expression levels and peak value of the frequency distribution were higher for the former than for the latter (Fig. 5). However, it was difficult to clearly classify each gene based solely on the mean expression level, since ePDF profiles of genes with moderate mean expression levels exhibit Gaussian, intermediate, or power-law like distribution.
Frequency distributions of average gene expression levels of gene groups exhibiting distinct ePDF profiles. Frequency distributions of average log gene expression levels in cases of Gaussian (blue), intermediate (black), and power law-like (red) distributions in 7-day-old (left) and 22-day-old (right) Arabidopsis. Differences in average log gene expression levels between Gaussian and intermediate, and between mixed and power law-like distributions were significant (P < 0.01, t test) at both plant ages.
Gene function was also correlated with ePDF profiles (Tables 2 and S4). For example, more than half of so-called essential genes46 showed a Gaussian distribution at four time points in young and old plants. Furthermore, genes encoding important intracellular components and organelles and those associated with electron transport in metabolic pathways tended to show Gaussian distributions. On the other hand, genes encoding transcription factors and nucleic acid-binding proteins mostly exhibited power law-like and intermediate distributions.
Discussion
More than 20 replicates of A. thaliana gene expression data at four harvest times of 7- and 22-day-old shoots were obtained and the ePDF profiles of each gene were analyzed. Most profiles could be fitted with a G-P function. Each gene could be classified by parameters of the G-P function fitting its ePDF profile of expression levels. There were three typical ePDF profiles—namely, Gaussian, power law-like, and intermediate.
The G-P function suggested that the various types of ePDF profile were highly correlated with network topology, particularly a feedback loop regulating gene expression; for instance, gene groups showing a power law-like distribution were predicted to be significantly influenced by a feedback mechanism, while this was rare for those exhibiting a Gaussian distribution.
In the present argument, Eq. (1) was obtained under the assumption that D/C is considerably smaller than any of the other values for the sake of simplicity. Equation (1) can play an important role in unambiguously classifying genes based on their ePDF profiles. On the other hand, the Gaussian distribution can be derived from Eq. (2) in cases not only such as (i) K ~ 0 and F/C > 0 (D/C ~ 0) as shown above, but also those such as (ii) F = 0 and D/C > 0. For some genes, for example those without any downstream, Eq. (2) with F = 0 and D/C > 0 may provide a more suitable model for the fluctuation of transcription levels.
There are other possible interpretations and mechanisms for a G-P function. In addition, Eq. (2) should be derived based on the general features of gene regulatory networks to confirm its validity as an effective model of gene regulatory dynamics. Relevant studies are now underway.
The ePDF profiles of genes were correlated with their average expression levels and functions; gene groups classified as being essential for survival tended to exhibit Gaussian distributions, whereas those encoding transcription factors and nucleic acid-binding proteins mostly followed a non-Gaussian (i.e., power law-like or intermediate) distribution. Furthermore, the expression levels of many genes classified as “unknown” exhibited power law-like distributions (Table S4), suggesting that their expression is predominantly modulated by feedback loops.
ePDF profiles of gene expression levels were inferred from publicly available RNA-seq data derived from 48-replicate experiments of S. cerevisiae36 in the same manner as in the present study. The G-P as well as the NB function fit the ePDF profiles of S. cerevisiae (Fig. S9). However, long-tailed power law-like ePDF profiles were not observed unlike for Arabidopsis genes for reasons that are unclear. There are many differences between Arabidopsis and S. cerevisiae: the former is a multicellular organism that undergoes differentiation, in which genes different genes are required by different cell types; it also exhibits circadian rhythm, with gene expression profiles showing variations over time; and finally, as a plant it has complex metabolic and gene regulatory networks that allow it to adapt to environmental stresses. Thus, Arabidopsis is expected to have more complex gene regulatory networks that include extensive feedback regulation than yeast; thus, some Arabidopsis genes can exhibit power-law like ePDF, unlike those of yeast.
Even when the analysis was performed using 24-replicate data randomly selected from the 48-replicate dataset, the results were qualitatively similar to those described above, except that the number of clusters differed (Fig. S10). Although the number of replicates in the present study was smaller than that used in the earlier report, our results reflect the essential properties of the ePDF profiles of Arabidopsis genes and are expected to apply to a larger number of replicates.
The present study showed that the expression levels of some Arabidopsis genes often exhibit power-law like distribution profiles that have not been reported in earlier RNA-seq analyses of various organisms. To obtain a long-tailed power law-like ePDF, a sufficient amount of rare data exhibiting a much higher expression levels than other genes must be observed. Such rare data have mostly been neglected as outliers in recent RNA-seq analyses with two or three replicates. On the other hand, the present RNA-seq analysis with more than 20 replications should provide a sufficient amount of such rare data that constitute the long-tail portion of ePDF profiles.
As shown in Fig. 1, the eCDF of transcription levels of each gene did not follow a smooth curve, but had a staircase-like slope. Accordingly, the obtained ePDF profile was too jagged for fitting and analysis. In the present study, the expression levels of different genes in the same cluster were mixed in order to simultaneously estimate and analyze ePDF profiles of each gene. However, it is possible that the present analysis contains some statistical bias. In order to test the validity of the present method and estimate such bias, it is necessary to analyze more data on the expression levels under identical conditions that can estimate the ePDF profile of each gene. Experiments involving larger replicates and additional statistical methods to analyze the present data are being developed and will be reported in the future.
In the present study, Arabidopsis genes were clearly separated into three classes. However, the underlying mechanistic and physiological reasons remain unclear; this issue should be analyzed in future.
This study mainly focused on the steady-state probability distributions of gene expression levels. However, many Arabidopsis genes are regulated by circadian rhythm. Future studies must therefore address the extent to which the present model can be generalized to dynamic situations. Furthermore, eq. (2) represents the expression of genes following a power law-like distribution, which is considered to exhibit intermittent temporal changes. This fact suggests a novel transcriptional burst mechanism8,9,47,48,49,50 for genes based on feedback regulation. Such dynamic features of gene expression warrant more detailed examination.
Materials and Methods
Plant growth conditions and RNA-seq
Seeds of A. thaliana (accession Col-0) were sown on Murashige and Skoog medium with 0.5% gellan gum. After incubation for 2 days at 4 °C in dark, the seeds were cultivated at 22 °C on a 12:12-h light/dark cycle. The whole aerial part of plants 7 or 22 days after germination was collected 1, 7, 13, and 19 h after the start of light period and immediately frozen in liquid nitrogen and stored on −20 °C until RNA extraction. Each individual plant was used as a sample for RNA-seq. Total RNA was extracted with the Maxwell 16 LEV Plant RNA kit (Promega, Madison, WI, USA). RNA-seq library preparation was performed as previously described51; seven lanes of single-end 50-bp sequencing of the library were analyzed using the Hiseq. 2000 and HiSeq. 2500 systems (Illumina, San Diego, CA, USA). Sequences were pre-processed, mapped, and quantified according to a previously described pipeline46. Fastq files were deposited into the DNA Data Bank of Japan Sequence Read Archive as accession no. DRA005887. Quantified expression data are available as Supplementary Information (Table S1).
Analysis of gene expression level variation bias
Owing to technical limitations, there was a time lag of several to 10 min during the harvesting of Arabidopsis leaf samples, potentially introducing a bias in the expression levels of some genes with respect to harvest time. In order to evaluate the variation bias in gene expression levels under each condition, we calculated the average gene expression levels from half of the samples harvested at early time points and half harvested at late time points. Gene expression level was regarded as stationary (unbiased) if the P value in the t test was >0.2.
Cluster analysis
k-Means cluster analysis using R software (http://www.r-project.org) was performed for normalized eCDF profiles using the Eucidian metric. The number of clusters was selected so as to minimize the Bayesian information criterion.
Data sources for gene classification
To classify each gene, the Gene Ontology Slim classification list was obtained from TAIR (http://www.arabidopsis.org). Data on essential genes were obtained from the SeedGenes Project (http://www.seedgenes.org/GeneList)52.
References
Elowitz, M. B., Levine, A. D., Siggia, E. D. & Swain, P. S. Stochastic Gene Expression in a Single Cell. Science 297, 1183–1186 (2002).
Furusawa, C., Suzuki, S., Kashiwagi, A., Yomo, T. & Kaneko, K. Ubiquity of Log-Normal Distributions in Intra-Cellular Reaction Dynamics. Biophysics 1, 25–31 (2005).
Golding, I., Paulsson, J., Zawilski, S. M. & Cox, E. C. Real-Time Kinetics of Gene Activity in Individual Bacteria. Cell 123, 1025–1036 (2005).
Kaern, M., Elston, T. C., Blake, W. J. & Collins, J. J. Stochasticity in Gene Expression: From Theories to Phenotypes. Nat. Rev. Genet. 6, 451–464 (2005).
Newman, J. R. et al. Single-Cell Proteomic Analysis of S. cerevisiae Reveals the Architecture of Biological Noise. Nature 441, 840–846 (2006).
Chang, H. H., Hemberg, M., Barahona, M., Ingber, D. E. & Huang, S. Transcriptome-Wide Noise Controls Lineage Choice in Mammalian Progenitor Cells. Nature 453, 544–547 (2008).
Konishi, T. et al. Coincidence Between Transcriptome Analyses on Different Microarray Platforms Using a Parametric Framework. PLoS One 3, e3555 (2008).
Taniguchi, Y. et al. Quantifying E. Coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells. Science 329, 533–538 (2010).
So, L. H. et al. General Properties of Transcriptional Time Series in Escherichia Coli. Nat. Genet. 43, 554–560 (2011).
Silander, O. K. et al. Genome-Wide Analysis of Promoter-Mediated Phenotypic Noise in Escherichia Coli. PLoS Genet. 8, e1002443 (2012).
Woods, H. A. Mosaic Physiology from Developmental Noise: Within-Organism Physiological Diversity as an Alternative to Phenotypic Plasticity and Phenotypic Flexibility. J. Exp. Biol. 217, 35–45 (2014).
Mitsui, K. et al. The Homeoprotein Nanog is Required for Maintenance of Pluripotency in Mouse Epiblast and ES Cells. Cell 113, 631–642 (2003).
Kaneko, K. Life: An Introduction to Complex Systems Biology. Springer, Berlin, Heidelberg (2006).
Kalmar, T. et al. Regulated Fluctuations in Nanog Expression Mediate Cell Fate Decisions in Embryonic Stem Cells. PLoS Biol. 7, e1000149 (2009).
Ochiai, H., Sugawara, T., Sakuma, T. & Yamamoto, T. Stochastic Promoter Activation Affects Nanog Expression Variability in Mouse Embryonic Stem Cells. Sci. Rep. 4, 7125 (2014).
Nagano, A. J. et al. Deciphering and Prediction of Transcriptome Dynamics Under Fluctuating Field Conditions. Cell 151, 1358–1369 (2012).
Sato, K., Ito, Y., Yomo, T. & Kaneko, K. On the Relation Between Fluctuation and Response in Biological Systems. Proc. Natl. Acad. Sci. USA 100, 14086–14090 (2003).
Blake, W. J., Kaern, M., Cantor, C. R. & Collins, J. J. Noise in Eukaryotic Gene Expression. Nature 422, 633–637 (2003).
Landry, C. R., Lemos, B., Rifkin, S. A., Dickinson, W. J. & Hartl, D. L. Genetic Properties Influencing the Evolvability of Gene Expression. Science 317, 118–121 (2007).
Choi, J. K. & Kim, Y. J. Epigenetic Regulation and the Variability of Gene Expression. Nat. Genet. 40, 141–147 (2008).
Choi, J. K. & Kim, Y. J. Intrinsic Variability of Gene Expression Encoded in Nucleosome Positioning Sequences. Nat. Genet. 41, 498–503 (2009).
Tirosh, I. & Barkai, N. Two Strategies for Gene Regulation by Promoter Nucleosomes. Genome Res. 18, 1084–109 (2008).
Lehner, B. Conflict Between Noise and Plasticity in Yeast. PLoS Genet. 6, e1001185 (2010).
Lehner, B. & Kaneko, K. Fluctuation and Response in Biology. Cell. Mol. Life Sci. 68, 1005–1010 (2011).
Bajic, D. & Poyatos, J. F. B. Noise and Plasticity in Eukaryotic Gene Expression. BMC Genom. 343, 1–11 (2012).
Singh, G. P. Coupling Between Noise and Plasticity in E. Coli. G3 (Bethesda) 3, 2115 (2013).
Hirao, K., Nagano, A. J. & Awazu, A. Noise–Plasticity Correlations of Gene Expression in the Multicellular Organism Arabidopsis Thaliana. J. Theo. Biol. 387, 13–22 (2015).
Salman, H. et al. Universal Protein Fluctuations in Populations of Microorganisms. Phys. Rev. Lett. 108, 238105 (2012).
Friedman, N., Cai, L. & Xie, X. S. Linking Stochastic Dynamics to Population Distribution: an Analytical Framework of Gene Expression. Phys. Rev. Lett. 97, 168302 (2006).
Mortazavi, A., Williams, B. A., McCue, K., Schaeffer, L. & Wold, B. Mapping and Quantifying Mammalian Transcriptomes by RNA-Seq. Nat. Methods 5, 621–628 (2008).
Nagalakshmi, U., Waern, K. & Snyder, M. RNA-Seq: A Method for Comprehensive Transcriptome Analysis. Curr. Protoc. Mol. Biol. Chapter 4, Unit 4. 11, 11–13 (2010).
Rapaport, F. et al. Comprehensive Evaluation of Differential Gene Expression Analysis Methods for RNA-Seq Data. Genome Biol. 14, R95 (2013).
Schurch, N. J. et al. How Many Biological Replicates Are Needed in an RNA-Seq Experiment and Which Differential Expression Tool Should You Use? RNA 22, 839–851 (2016).
Marioni, J. C., Mason, C. E., Mane, S. M., Stephens, M. & Gilad, Y. RNA-Seq: an Assessment of Technical Reproducibility and Comparison with Gene Expression Arrays. Genome Res. 18, 1509–1517 (2008).
Robinson, M. D. & Smyth, G. K. Small-Sample Estimation of Negative Binomial Dispersion, with Applications to SAGE Data. Biostatistics 9, 321–332 (2008).
Gierliński, M. et al. Statistical Models for RNA-Seq Data Derived from a Two-Condition 48-Replicate Experiment. Bioinformatics 31, 3625–3630 (2015).
Smith, G. R. & Birtwistle, M. R. A Mechanistic Beta-Binomial Probability Model for mRNA Sequencing Data. PLoS One 11, e0157828 (2016).
Karthik, D., Stelzer, G., Gershanov, S., Baranes, D. & Salmon-Divon, M. Elucidating Tissue Specific Genes Using the Benford Distribution. BMC Genomics 17, 595 (2016).
Shen, X., Pettersson, M., Rönnegård, L. & Carlborg, Ö. Inheritance Beyond Plain Heritability: Variance-Controlling Genes in Arabidopsis Thaliana. PLoS Genet. 8, e1002839 (2012).
Maruyama-Nakashita, A. et al. Identification of a Novel Cis-Acting Element Conferring Sulfur Deficiency Response in Arabidopsis Roots. Plant J. 42, 305–314 (2005).
Nemhauser, J. L., Hong, F. X. & Chory, J. Different Plant Hormones Regulate Similar Processes Through Largely Nonoverlapping Transcriptional Responses. Cell 126, 467–475 (2006).
Kilian, J. et al. The AtGenExpress Global Stress Expression Data Set: Protocols, Evaluation and Model Data Analysis of UV-B Light, Drought and Cold Stress Responses. Plant J. 50, 347–363 (2007).
Goda, H. et al. The AtGenExpress Hormone and Chemical Treatment Data Set: Experimental Design, Data Evaluation, Model Data Analysis and Data Access. Plant J. 55, 526–542 (2008).
Less, H. & Galili, G. Principal Transcriptional Programs Regulating Plant Amino Acid Metabolism in Response to Abiotic Stresses. Plant Physiol. 147, 316–330 (2008).
Wittenberg, T. A., Tzin, V., Angelovici, R., Less, H. & Galili, G. Deciphering Energy-Associated Gene Networks Operating in the Response of Arabidopsis Plants to Stress and Nutritional Cues. Plant J. 70, 954–966 (2012).
Kamitani, M., Nagano, A. J., Honjo, M. N. & Kudoh, H. RNA-Seq Reveals Virus-Virus and Virus-Plant Interactions in Nature. FEMS Microbiol. Ecol. 92, fiw176 (2016).
Munsky, B., Neuert, G. & van Oudenaarden, A. Using Gene Expression Noise to Understand Gene Regulation. Science 336, 183–187 (2012).
Sanchez, A. & Golding, I. Genetic Determinants and Cellular Constraints in Noisy Gene Expression. Science 342, 1188–1193 (2013).
Jones, D. L., Brewster, R. C. & Phillips, R. Promoter Architecture Dictates Cell-to-Cell Variability in Gene Expression. Science 346, 1533–1536 (2014).
Fujita, K., Iwaki, M. & Yanagida, T. Transcriptional Bursting is Intrinsically Caused by Interplay Between RNA Polymerases onDNA. Nat. Comm. 7, 13788 (2016).
Nagano, A. J., Honjo, N. H., Mihara, M., Sato, M. & Kudoh, H. Detection of Plant Viruses in Natural Environments by Using RNA-Seq. Methods Mol. Biol. 1236, 89–98 (2015).
Meinke, D., Muralla, R., Sweeney, C. & Dickerman, A. Identifying Essential Genes in Arabidopsis Thaliana. Trends Plant Sci. 13, 483–491 (2008).
Acknowledgements
The authors thank F. Kobayashi for assistance in RNA preparation, and H. Kudoh and S. Takada for helpful discussions. This research was partly supported by the Platform Project for Support in Japan Agency for Medical Research and Development (to A.A.); a Grant-in-Aid for Scientific Research on Innovative Areas “Integrated Analysis of Strategies for Plant Survival and Growth in Response to Global Environmental Changes” from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (no. 25119718 to A.A.); a Grant-in-Aid for Scientific Research on Innovative Areas “Initiative for High-Dimensional Data-Driven Science through Deepening of Sparse Modeling” from MEXT (no. 26120525 to A.A.); a MEXT KAKENHI (no. 17K05614 to A.A.); Japan Science and Technology Agency Core Research for Evolutional Science and Technology (no. JPMJCR15O2 to A.J.N.); and Japan Society for the Promotion of Science KAKENHI (nos. JP16H06171 and JP16H01473 to A.J.N.).
Author information
Authors and Affiliations
Contributions
A.A. analyzed the experimental data, performed mathematical modeling, wrote the manuscript, and coordinated the study. T.T. analyzed the experimental data. M.K. and A.T. performed measurements. A.J.N. designed the experiments, performed measurements, and wrote the manuscript.
Corresponding author
Ethics declarations
Competing Interests
The authors declare no competing interests.
Additional information
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Awazu, A., Tanabe, T., Kamitani, M. et al. Broad distribution spectrum from Gaussian to power law appears in stochastic variations in RNA-seq data. Sci Rep 8, 8339 (2018). https://doi.org/10.1038/s41598-018-26735-4
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/s41598-018-26735-4
- Springer Nature Limited
This article is cited by
-
Comparison and evaluation of statistical error models for scRNA-seq
Genome Biology (2022)