Abstract
Microtubule (MT) filaments, composed of α/β-tubulin dimers, are fundamental to cellular architecture, function and organismal development. They are nucleated from MT organizing centers by the evolutionarily conserved γ-tubulin ring complex (γTuRC). However, the molecular mechanism of nucleation remains elusive. Here we used cryo-electron tomography to determine the structure of the native γTuRC capping the minus end of a MT in the context of enriched budding yeast spindles. In our structure, γTuRC presents a ring of γ-tubulin subunits to seed nucleation of exclusively 13-protofilament MTs, adopting an active closed conformation to function as a perfect geometric template for MT nucleation. Our cryo-electron tomography reconstruction revealed that a coiled-coil protein staples the first row of α/β-tubulin of the MT to alternating positions along the γ-tubulin ring of γTuRC. This positioning of α/β-tubulin onto γTuRC suggests a role for the coiled-coil protein in augmenting γTuRC-mediated MT nucleation. Based on our results, we describe a molecular model for budding yeast γTuRC activation and MT nucleation.
Similar content being viewed by others
Main
Microtubule (MT) filaments are essential cellular structures1. MT filaments assemble the mitotic spindle apparatus responsible for chromosome segregation, whereas the MT-based cytoskeletal network mediates dynein and kinesin-based intracellular transport. MTs are formed by the dynamic oligomerization and depolymerization of α/β-tubulin dimers in a head-to-tail fashion, with α-tubulin exposed at the ‘minus’ end of MTs and β-tubulin at the more dynamic MT ‘plus’ end2. In vitro, de novo α/β-tubulin polymerization to form MTs is kinetically limited and occurs only at high tubulin concentrations3,4,5, whereas in cells the evolutionarily conserved γTuRC templates efficient MT nucleation at MT organizing centers (MTOCs) such as the vertebrate centrosome and yeast spindle pole body (SPB) that assemble mitotic spindles6,7,8,9. Previous structural studies of native Xenopus laevis10 and Homo sapiens11,12 γTuRCs, as well as recombinant H. sapiens13,14,15 and Saccharomyces cerevisiae16,17,18 γTuRCs showed that the complex forms a large conical structure. S. cerevisiae γTuRC is built from a helical repeat of seven ‘V’-shaped copies of the tetrameric γ-tubulin small complex (γTuSC)18,19, composed of Spc97/GCP2 (SPB component 97/γ-tubulin complex 2) and Spc98/GCP3 subunits each bound to a γ-tubulin molecule16,17. Spc110 (vertebrate CDK5RAP2 ortholog) links adjacent γTuSCs via its centrosomin motif 1 (CM1), and Spc110 is also required for γTuRC recruitment and assembly at the SPB18,20,21,22,23. γTuRC contains 14 γ-tubulin molecules, but it templates nucleation of 13-protofilament (PF) MTs. Most vertebrate γTuRCs exhibit open conformations and reveal a geometrical mismatch between the γ-tubulin ring and the MT lattice, and are poor templates for MT nucleation in vitro. Notably, the reported higher affinity of γ-tubulin for α/β-tubulin, compared to α/β-tubulin self-association, is neither sufficient to nucleate MTs nor for γTuRC to transition to the closed conformation7. Structures of yeast γTuSC filaments exhibit a spectrum of conformational states, and appear to sample ‘open’ and near ‘closed’ conformations in solution18. An engineered yeast γTuSC filament stabilized with disulfide cross-links helped to lock a near ‘closed’ conformation of the γTuSC filament17. Disulfide cross-linking also enhanced MT nucleation efficiency of yeast γTuRC two- to threefold, yet the α/β-tubulin concentrations required for efficient nucleation were still above physiological levels17. This suggests that matching the geometry of γTuRC to the geometry of MT is an important but perhaps not sufficient step to control MT nucleation. Reconstitution and cell biology experiments also defined a critical role for MT polymerases in γTuRC-mediated MT nucleation12,24,25,26,27. These studies present an intriguing puzzle: how is efficient MT nucleation in cells achieved from a seemingly imperfect template? A number of mechanisms have been proposed to explain the high efficiency of MT nucleation at endogenous γTuRC: activation by effector molecules such as MT polymerases, tubulin lattice-mediated activation and phosphorylation-driven activation7. It is, however, unknown to what degree and how each of these mechanisms, if at all, contributes to γTuRC activation and MT nucleation.
In this article, to understand how γTuRC mediates nucleation of exclusively 13-PF MTs, we determined the structure of the active native γTuRC as part of enriched yeast mitotic spindles using cryo-electron tomography (cryo-ET). In our structure, γTuRC is observed as a perfect geometric template bound at the minus end of the complete MT lattice. We describe two additional novel cryo-ET densities in our reconstructions: (1) long coiled-coil-like proteins spanning Spc98, γ-tubulin and the first α/β-tubulin array of the MT lattice, where these coiled coils staple the first row of α/β-tubulin to the γ-tubulin ring, and (2) a luminal bridge density that probably stabilizes the oligomeric state of the γTuRC capping MT minus ends. Our structure of a native γTuRC capping a MT expands our understanding of the molecular mechanism of γTuRC-mediated MT nucleation.
Results
γTuRC forms a perfect helical template for MT nucleation
We prepared enriched endogenous S. cerevisiae (strain NCYC74) SPBs, the MTOCs in yeast, for cryo-ET studies (Fig. 1a,b, Extended Data Fig. 1a and Supplementary Video 1). The protein sequences of the γTuRC components of this strain are at least 95% identical to the canonical S. cerevisiae S288c. Canonical structures such as the outer, intermediate and central plaques28,29, in addition to ~25 nuclear MTs (nMTs) per SPB, were readily observed in the SPB tomograms (Fig. 1b and Supplementary Video 1). This matches on average the number of MTs per spindle pole reported for intact S. cerevisiae cells30, suggesting that the majority of MTs remain stably associated with the SPBs during enrichment. Additionally, the MT lattices are unperturbed (as discussed later during MT subtomogram averaging), and virtually all MTs are attached via their minus ends, to the inner plaque of the SPB (Fig. 1b).
a, A negative-stain electron micrograph of the S. cerevisiae SPBs forming a bipolar spindle, a typical structure observed following the enrichment procedure. Similar spindle structures were observed in more than ten independent SPB enrichment preparations. b, Left: a slice through a representative tomogram showing outer, central and inner plaques of the intact SPB, and a capped MT minus end (circled). Right: slices through denoised tomographic reconstructions of spindle MTs. MT-capping γTuRCs and Spc110 coiled coils connect the MTs to the SPB. The MT lattice and γTuRC caps are intact. c, A consensus cryo-ET reconstruction of the γTuRC capping the α/β-tubulin lattice after equalizing density thresholds using Occupy52. Scale bar as in b. d, A molecular model of the γTuRC bound to the MT minus end. Spokes are numbered as in Extended Data Fig. 2a, and a γTuSC unit is highlighted in a red box.
We performed subtomogram averaging of the SPB-proximal MT minus ends, capped by γTuRC, and the MT lattice itself using a tailored processing pipeline (Extended Data Fig. 1b–d). We determined a consensus reconstruction of the native yeast γTuRC, capping the α/β-tubulin lattice at the minus end, to a global resolution of 9.2 Å (Fig. 1c,d, Table 1, Extended Data Fig. 1b,c and Supplementary Video 1). Subboxing of the γTuRC at γTuSC positions, followed by focused refinements, improved the resolution to 7–9 Å resolution (Extended Data Fig. 1b,c). In parallel, we also reconstructed the native yeast MT lattice at 6.6 Å resolution (Table 1 and Extended Data Fig. 1b,c). Using available high-resolution structures of yeast γTuSC18,31 and MTs32, we generated a complete molecular model of γTuRC capping mitotic spindle MT minus ends (Fig. 1c,d and Supplementary Video 1). In our tomograms and during subtomogram averaging, we did not observe γTuRCs devoid of MTs or incomplete γTuRC complexes, nor did we observe detached γTuRC molecules or uncapped MTs, suggesting that only complete γTuRC complexes attached to the SPB nucleate MTs in the native system. This is consistent with observations that recruitment of γTuRC to the SPBs and MT nucleation are closely coupled and regulated processes16,20,22,23,33.
In our γTuRC reconstruction, seven γTuSC protomers assemble side-on to generate a pseudo-helical γTuRC at the base of the MT lattice, consisting of 14 spokes (formed by Spc98:γ-tubulin and Spc97:γ-tubulin of γTuSC promoters) (Figs. 1c,d and 2a, and Extended Data Fig. 2a). As spoke 14 is positioned directly above spoke 1, γTuRC presents 13 γ-tubulin molecules for MT nucleation (spokes 2–14) (Extended Data Fig. 2a,b). The stoichiometry of the native budding yeast γTuRC is identical to that of extracted vertebrate γTuRCs, even though individual yeast γTuSC protomers do not assemble into a higher-order defined ring in solution16,17,19. The geometry of the active γTuRC matches that of a 13-PF MT lattice perfectly, with a helical rise of 84 Å, a helical twist of ~28° and a width of 220 Å (Fig. 2a). Overall, our active γTuRC structure exhibits a substantially different geometry compared to the previously reported open structure of the recombinant yeast γTuSC filament17,18, and other structures of inactive vertebrate γTuRCs10,11,12 (Fig. 2b).
a, Top-down and side views of the native yeast γTuRC core complex (left) and the MT lattice (right) showing an identical helical geometry. The MT lattice was modeled on the basis of our 6.6 Å reconstruction of the native yeast MT lattice (Extended Data Fig. 1a). ‘(b)’ refers to the view shown in b. b, The native, closed and active yeast γTuRC structure was superimposed onto the inactive human γTuRC complex (PDB ID: 6V6S) (top) and single helical turn of the yeast γTuSC filament (PDB ID: 7m2x) (bottom), showing that γTuRC undergoes extensive conformational changes to match MT geometry and thus template MT nucleation. c, Left: a comparison of γTuSC geometries between unassembled γTuSC modules (PDB ID: 7m2z) and γTuSC modules in the context of active γTuRC. Right: a comparison of γTuSC geometries between γTuSC within the open-inactive γTuSC filament (PDB ID: 7m2x) and γTuSC in the context of active γTuRC. γ-tubulin pairs of each γTuSC module straighten up to adopt 13-PF MT geometry. Coordinates were superimposed on the Spc98GRIP1 domain. d, γ-Tubulin pairs straightening is accommodated by Spc98GRIP2 straightening and Spc97GRIP2 bending. PDBs were superimposed on the Spc98GRIP1 domain. e, A view of the interfaces at the γTuRC:MT seam, focusing on the electrostatic α-tubulin:γ-tubulin as well as Spc98:β-tubulin interfaces. The insets on the right show the α-tubulin:γ-tubulin (i) and Spc98:β-tubulin (ii) interfaces as electrostatic surfaces.
Relative to unassembled yeast γTuSC modules, and γTuSC modules assembled into the open γTuSC filament17,18, the closed ring conformation of native γTuRC requires tightening and tilting of the two γ-tubulin subunits of each γTuSC module, so as to match the lateral α/β-tubulin geometry of the MT lattice (Fig. 2c). This γ-tubulin interface reorganization is facilitated by straightening of the gamma ring protein 2 (GRIP2) domain of Spc98 and bending of the GRIP2 domain of Spc97 (Fig. 2d). γTuRC ring closure is then accommodated by leaving an empty gap above the Spc97:γ-tubulin spoke 1 of γTuSC-1 (Extended Data Fig. 2b). Comparing our structure to human γTuRC, we observed that the first four GCP2/3 modules of human γTuRC match a 13-PF MT lattice, but the GCP4/5:GCP4/6 nucleation core as well as the last GCP2/3 module have to move inwards by ~50 Å to match the MT lattice geometry (Fig. 2b)11. The closed geometry of native γTuRC is similar but not identical to that of a recombinant γTuSC filament trapped in a closed state by engineered disulfides in γ-tubulin18. Ring closure induced by the γ-tubulin disulfide enhanced MT nucleation efficiency in vitro17, a finding in support of the idea that, by matching the geometry of a MT lattice, γTuRC templates MT nucleation.
Ring closure at the γTuRC seam, which aligns with the MT lattice seam, is achieved through interactions between Spc98:γ-tubulin of γTuSC-7 and the first α/β/α-tubulin row of the MT lattice (Fig. 2e). The β-tubulin:Spc98 interface (Fig. 2e, ii) at the seam is formed mainly through salt bridge interactions between a basic Spc98GRIP2 patch and acidic peripheral β-tubulin helices. The lateral α-tubulin:γ-tubulin interface at the γTuRC seam is also formed through electrostatic interactions (Fig. 2e, i) and reminiscent of the lateral α:β-tubulin interface at the MT lattice seam, with a single lateral contact site—a loop from the α-tubulin globular fold connecting α-tubulin and γ-tubulin. This is in contrast to two lateral contacts between α/β-tubulin molecules in the MT lattice, but similar to single-contact interactions between γ-tubulin molecules in native γTuRC (Extended Data Fig. 2c). Overall, the longitudinal γ:α-tubulin interface at the nucleated γTuRC is almost identical to the β:α-tubulin interface within the MT lattice (Extended Data Fig. 2c)34. At a resolution of 7 Å in this region, we cannot exclude that there are other subtle differences between the γ:α-tubulin and β:α-tubulin interfaces.
The essential Spc110 N-terminus forms a dimeric coiled coil (Spc110NCC) at the base of six γTuSC modules (γTuSC-1 to γTuSC-6) (Fig. 1c,d). The Spc110NCC bound to the last γTuSC of the ring (γTuSC-7, spokes 13 and 14) also binds to the first γ-tubulin of γTuSC-1 (spoke 1), probably further stabilizing γTuRC ring closure (Fig. 1d). For all γTuSC modules, Spc110 then threads over the surface of an Spc98–Spc97 protomer and links adjacent γTuSC modules through the highly conserved CM1 motif (Spc110CM1) that binds across the GRIP2 domains at each inter-γTuSC interface18 (Extended Data Fig. 3a). Our reconstruction of the native γTuRC and its interactions with Spc110 matches a higher-resolution single-particle reconstruction of γTuSC filaments bound to Spc110 (ref. 18). In addition to the known interaction interfaces, at lower isosurface thresholds, we also observe Spc110NCC of γTuSC-1 to γTuSC-6 converging into a single filament at the base of the γTuRC, consistent with Spc110 oligomerization being an important step during yeast γTuRC formation20,21 (Extended Data Fig. 3b).
Three-dimensional (3D) classification of the MT subtomogram volumes against 11–16 PF reference structures revealed that the native MT lattice contains almost exclusively 13 PFs (97%, Extended Data Fig. 3c) consistent with γTuRC presenting 13 γ-tubulin molecules for MT nucleation. This is in contrast with yeast MTs nucleated in vitro using recombinant α/β-tubulin, where a highly variable PF number is observed, with 14 PFs being the most abundant32, although in agreement with native yeast α/β-tubulin MTs nucleation in vitro35.
Coiled-coil proteins stabilize the first row of α/β-tubulin
The consensus reconstruction and the subboxed map revealed repetitive densities for a coiled-coil protein that spans each Spc98:γ-tubulin pair and the first row of α/β-tubulin (Figs. 1c and 3a,b). These are absent from all previously reported vertebrate and yeast γTuRC structures. The coiled-coil protein binds the Spc98 GRIP2 domain, γ-tubulin and α-tubulin, where the coiled-coil end flares: the density for one of the α-helices terminates while the other α-helix continues and interacts with the β-tubulin. The interface between the coiled coil and γTuSC:α/β-tubulin is shallow and is formed primarily by numerous highly conserved acidic residues on the γ-tubulin (Fig. 3b) and α-tubulin surfaces. The coiled coils appear to stabilize α:β-tubulin dimers at Spc98-bound γ-tubulin, effectively positioning the first α:β-tubulin dimers of the MT lattice. Notably, no coiled coils are bound across the Spc97:γ-tubulin:α-tubulin surface. A coiled coil bound to Spc97 would probably promote α:β-tubulin binding to the first Spc97:γ-tubulin spoke (Extended Data Fig. 2b, spoke 1), which would interfere with γTuRC ring closure, potentially explaining why coiled-coil binding to Spc97 could be biologically undesirable.
a, A subboxed cryo-ET map at 8 Å, and molecular model of a γTuSC bound to the first two rows of α/β-tubulin. b, A model and map of the coiled-coil interaction with Spc98, γ-tubulin and α/β-tubulin. The crystal structure of the coiled-coil domain of Stu2 (ref. 38) fits the coiled-coil density in the subtomogram average, as shown in b. c, The tub4-401 mutant (γ-tubulin mutant D421R, E425R and E428R), predicted to disrupt the coiled-coil interaction with γ-tubulin, grows normally at 23 °C but shows growth defects at 36 °C. TUB4WT, wild-type γ-tubulin. d, Intra- and intermolecular cross-links observed during CLMS experiments of the enriched SPB preparation. Proteins are shown as nodes, labeled with their respective protein names, and color-coded according to their protein group affiliation (as indicated above). Cross-links identified from the listed protein groups through CLMS analysis of the enriched SPB preparation are depicted. Self-links are represented by loops on the nodes, while heteromeric cross-links are denoted by lines connecting the nodes. γTuRC proteins are labeled in dark cyan. Two coiled-coil proteins, Stu2 and BRE1, and one globular protein (Ima1) were found to be cross-linked to γTuRC proteins, of which Stu2, known to be enriched at SPBs, is shown in red.
The observation that the coiled-coil protein binds exclusively to Spc98:γ-tubulin:α-tubulin, and not to Spc97:γ-tubulin:α-tubulin, implies there are Spc98-specific structural features that underlie this specificity. First, whereas a basic patch on the Spc98 GRIP2 domain faces the coiled-coil protein, an acidic patch is exposed on the corresponding GRIP2 domain of Spc97 (Extended Data Fig. 4a). Second, Spc97GRIP2 exposes a large unstructured loop (residues 720–753, ‘GRIP2Loop’ (Extended Data Fig. 4b), absent in Spc98, which might prevent the coiled coil from binding. Last, the acidic carboxy-terminal region of γ-tubulin (γ-tubulinCTR) interacts differently with the Spc97GRIP2 and Spc98GRIP2 domains in our γTuRC reconstruction. Alphafold2 modeling indicated that the conserved ‘DSYL’ motif of γ-tubulinCTR forms a small interface with Spc97GRIP2 and Spc98GRIP2, a prediction supported by high-resolution structures of γTuSC18 (Extended Data Fig. 4b,c). C-terminal of the ‘DSYL’ motif, γ-tubulinCTR is predicted to form more extensive interactions with the Spc98GRIP2 residues (but not with Spc97GRIP2) through hydrophobic interactions. These Spc98-specific interfaces would direct γ-tubulinCTR toward the coiled-coil density (Extended Data Fig. 4d). These predictions are supported by cryo-ET density spanning Spc98GRIP2 in our subtomogram average (Extended Data Fig. 4b–d). Overall, Spc98 presents specific structural features that could confer specificity for coiled-coil binding.
To investigate the function of the coiled-coil protein, we tested if the coiled-coil interaction with the γTuRC is functionally important. We reversed the charge of a conserved acidic patch on γ-tubulin (γ-tubulin D421R, E425R and E428R) that directly engages the coiled coil and that is the largest interface with the coiled-coil density (Fig. 3b). We observed a temperature-sensitive phenotype in a yeast strain with a mutation in the γ-tubulin gene (tub4-401), indicating that the γ-tubulin:coiled-coil interface is important for cellular function (Fig. 3c). Due to likely pleiotropic effects, no mutants of α/β-tubulin were tested. This result indicated that the interaction between the coiled-coil staple and γ-tubulin:Spc98 is functionally important for MT formation and/or stability.
Due to the limited resolution, it was not possible to establish the identity of the coiled-coil protein from the cryo-ET maps. To define candidate proteins that could account for the coiled-coil density, we subjected our enriched SPB sample to mass spectrometry (MS) (Supplementary Data 1). Consistent with our structural work, α- and β-tubulin were the most enriched proteins, with abundant SPB components such as Spc110 and other components of the γTuRC also enriched (Extended Data Fig. 5a). We then focused on proteins that localize to the spindle MTs and contain a coiled coil of >10 nm. Based on these criteria Stu1, Bim1 (binding to MTs 1) and a few other proteins could be excluded, since they either lack a predicted coiled coil or have coiled coils that are too short to occupy the coiled-coil density in our reconstruction. However, most other known MT-associated proteins (MAPs) such as the kinesins Kip1 and Cin8, the MT polymerase Stu2 (vertebrate XMAP215 ortholog), Bik1 and many components of the kinetochore are present in the enriched SPB sample, are known to localize close to the SPBs in cells and have a coiled-coil domain of at least 10 nm. Spc110 is also a possible candidate; it contains coiled-coil regions essential for cellular function, albeit none N-terminal of the CM1 helix.
To further define the coiled-coil protein, we performed cross-linking mass spectrometry (CLMS) analysis of our enriched SPB preparation, which revealed many cross-links that are consistent with known interactions of the SPB plaques, γTuRC and kinetochore (Fig. 3d)16,29,36. Apart from known components of the γTuRC, CLMS did not detect any other proteins interacting directly with either Spc98 or γ-tubulin. However, of three newly identified cross-linked proteins to Spc110, two are coiled-coil proteins: Bre1 and Stu2. Furthermore, of these two proteins, only Stu2 is enriched at SPBs, cross-linking near the Spc110 N-terminus, although not via its coiled coil (Fig. 3d and Extended Data Fig. 5a,b). Stu2 is an essential protein in yeast that co-localizes with γ-tubulin in cells, even in the absence of MTs, suggesting that its interaction with γTuRC might be independent of α:β-tubulin37. Stu2 has a parallel, homodimeric 15 nm coiled coil, which is important for its function in cells38, as well as two tumor overexpressed gene (TOG) domains that bind free α/β-tubulin and stimulate MT polymerization39,40. In follow-up experiments, we were unable to detect meaningful cross-links between recombinant Stu2 and recombinant budding yeast γTuRC (composed of Spc97, Spc98, Tub4 and Spc110 amino acids 1–400, but without yeast tubulin) using CLMS. In addition, we could not detect an interaction between recombinant γTuRC and Stu2 using gel-filtration reconstitution experiments. Overall, we attempted to narrow down the list of potential coiled-coil candidates, and found a plausible hit, but its precise identity remains unknown and requires further investigation.
γTuRC luminal density bridges γTuSC protomers
In addition to the coiled-coil densities, we observe an additional density feature in the lumen of native γTuRC, unaccounted for by existing yeast γTuRC models, but reminiscent of the luminal bridge (γTuRCLB) described in higher eukaryotes41,42 (Figs. 1c and 4a,b). The γTuRC luminal density is compact and connects multiple γTuSC modules from the inside of the lumen, interacting specifically across inter-γTuSCs interfaces of γTuSC-3 to γTuSC-5. Deletion of the γTuRCLB from higher eukaryotes results in incomplete γTuRC assembly, suggesting that it helps stabilize γTuRC oligomerization13,43, while Mzt1 (a component of the γTuRCLB) has been shown to regulate γTuRC-mediated MT nucleation43. We do not observe density for actin, present in vertebrate γTuRC10,11,12, in the lumen of the S. cerevisiae γTuRC. This is consistent with actin not being present in the S. cerevisiae nucleus44.
The γTuRCLB in vertebrate γTuRC is formed by Mzt1 intertwined with either the GCP3 or GCP6 (Spc98 homologs) N-terminal helical domains (GCP3NHD and GCP6NHD)41,42,45 (Extended Data Fig. 6a). However, the mzt1 gene is absent from S. cerevisiae, and we did not detect other small α-helical proteins in our MS analysis (Extended Data Fig. 5a and Supplementary Data 1). S. cerevisiae Spc98 has a structured N-terminal α-helical domain (Spc98NHD), as predicted by AlphaFold2, not visible in the inactive recombinant yeast γTuSC filaments. We determined a crystal structure of the Spc98NHD at 1.9 Å resolution (Table 2), revealing a compact α-helical bundle nearly identical to the AlphaFold2 prediction (Fig. 4b). Interestingly, S. cerevisiae Spc98NHD is more compact than the extended GCP3NHD/GCP6NHD folds alone and thus more similar in size and fold to half of the composite Mzt1-GCP3NHD/GCP6NHD structures (Extended Data Fig. 6a). This observation could explain the absence of Mzt1 in budding yeast as Spc98NHD is more compact and its structure by itself makes up for the lack of Mzt1. The γTuRC luminal bridge density can in principle accommodate a trimer of the Spc98NHD crystal structure, in agreement with an AlphaFold2 prediction of a trimeric Spc98NHD oligomer (head-to-tail) (Fig. 4b and Extended Data Fig. 6b). Additionally, we observe densities connecting the N-terminal base of Spc98 and the luminal γTuRC density (Extended Data Fig. 6c), consistent with our preliminary annotation of the luminal bridge as a trimer of Spc98NHD. It is likely that the other four Spc98NHD domains are not bound in the γTuRC lumen, and are averaged out due to the flexible linker between Spc98GRIP1 and Spc98NHD, similar to γTuRC in higher eukaryotes10,11,12. Consistent with our model, deletion of Spc98NHD in S. cerevisiae impaired cell growth under stress and reduced γTuRC oligomerization propensity in vitro21. Overall, we show that the luminal bridge is a feature of all reported native γTuRC structures, probably facilitating formation of the complete γTuRC architecture, and that the luminal bridge remains associated with the active γTuRC at capped MT minus ends at the budding yeast γTuRC.
The endogenous MT lattice is in the compacted state
We reconstructed the native yeast MT lattice to 6.6 Å resolution (Fig. 5a,b and Extended Data Fig. 1b). The MT lattice in our tomograms is densely decorated with MAPs that could not be resolved during consensus map refinement due to conformational heterogeneity. The native yeast MT lattice is in a partially compacted state compared to structures of yeast MT reconstituted in vitro32, suggesting that the native nucleotide state as well as MAPs result in a well-defined MT lattice spacing in cells. The resolution of our reconstruction did not permit the unambiguous assignment of the nucleotide state of α/β-tubulin.
a, A model and 6.6 Å cryo-ET reconstruction of native yeast spindle MTs. Measurement of the distance between adjacent α/β-tubulins shows that the MT lattice is in the compacted state, when compared to MT lattice structures determined in vitro32. b, A focused view on the individual α/β-tubulin unit with molecular model overlayed with the reconstructed cryo-ET map.
Discussion
Since the discovery of γTuRC as the universal MT nucleator almost 30 years ago6,46, the precise molecular mechanism of γTuRC-mediated MT nucleation has remained elusive. We describe a 7–9 Å resolution structure of a native γTuRC capping nMT minus ends at the MTOC, obtained through subtomogram averaging. We observe γTuRC in a closed conformation, with conformational changes of the GRIP2 domains in each of the γTuRC spokes, presenting 13 γ-tubulin molecules as a perfect geometric template for MT nucleation. Recent studies of active human γTuRC bound to the MT lattice illustrate that this geometric templating mechanism is conserved between human and yeast cells47,48. Arresting features of our native γTuRC structure are the long coiled-coil proteins bound to every Spc98:γ-tubulin:α-tubulin interface, as well as the luminal bridge density.
We observe that the luminal density in our native budding yeast γTuRC reconstruction is reminiscent of the Mzt1:GCP3/6NHD luminal bridge found in higher eukaryotes. This observation indicates that the luminal bridge is a feature of all reported native γTuRC structures, probably facilitating formation of the complete γTuRC architecture. In budding yeast, however, there is no Mzt1 homolog, nor nuclear actin, and we propose that the luminal density corresponds to an oligomer of the compact Spc98NHD helical bundle (Fig. 4a). Whereas we observe the luminal density in the native γTuRC capping MT minus ends, the luminal bridge, including actin, is present in the open conformation of the human γTuRC but appears to be displaced from the fully closed human γTuRC after MT nucleation48. While the more substantial Mzt1:GCP3/6NHD:actin luminal bridge was proposed to sterically hinder human γTuRC closure upon MT nucleation, the more compact luminal density in budding yeast γTuRC can be accommodated upon closure, as seen in our reconstructions.
At least two functions can be envisaged for the coiled-coil protein. (1) The coiled coil might stabilize attachment of the nMT minus end to γTuRC to prevent nMT detachment and dissociation throughout the cell cycle. Unlike vertebrate MTOCs, yeast γTuRC stably caps MT minus ends at the SPB during most of the cell cycle. (2) The coiled-coil protein might be involved in the process of MT nucleation by directly recruiting the first row of α/β-tubulin to γTuRC. In vitro, γTuRC activation and MT nucleation from γTuRC requires either four25 or seven12 α/β-tubulin heterodimers bound to the γ-tubulin ring. It is plausible that the coiled-coil protein facilitates this step by stabilizing up to seven α/β-tubulin heterodimers longitudinally (one for every Spc98:γ-tubulin spoke). Importantly, these two mechanisms of action are not mutually exclusive. Unambiguous identification of the coiled-coil protein in future studies will allow dissection of its function and will also provide insights into whether similar mechanisms exist in other organisms. Whether the coiled-coil domains are bound to and function at the cytoplasmic γTuRC remains an open question. Lastly, whether ‘coiled-coil’ proteins stabilize longitudinal interactions of the first row of α/β-tubulin in native higher eukaryote γTuRC remains to be discovered. In reconstituted human γTuRC, an unidentified ‘latch’ was observed at the MT base, laterally bridging γ-tubulin at spoke 1 and β-tubulin at spoke 14 at the seam base of a ‘closing’ γTuRC intermediate. This observation indicates that additional regulatory components play a role in human γTuRC activation and MT nucleation48. Interestingly, the latch density is no longer observed in reconstructions of fully closed human γTuRC, and no such density was observed in our reconstructions of closed budding yeast γTuRC either. In our reconstruction, γTuRC closure at the seam base is stabilized solely by electrostatic interfaces between Spc98GRIP2: β-tubulin and γ-tubulin:α-tubulin (Fig. 2e).
Our structural findings together with the wealth of prior functional and biochemical data allow us to propose two plausible pathways of MT nucleation by yeast γTuRC (Fig. 6). Spc110 recruits individual γTuSCs to the SPB, which facilitates their oligomerization into a complete γTuRC, an activity that is promoted by Spc98NHD, forming the luminal bridge, as well as the Spc110CM1 helix, stabilizing adjacent γTuSCs specifically at the SPB (Fig. 6, i)18. Fully or partially assembled γTuRC can recruit the coiled-coil protein, while in an equilibrium of open and closed conformations (Fig. 6, inset ii-a), or transiently bind α/β-tubulin though longitudinal interactions with γ-tubulin (Fig. 6, inset ii-b). γTuRC together with the coiled-coil protein then stabilizes attachment of up to seven α/β-tubulin dimers on top of the central ring scaffold (Fig. 6, inset iii-a/b). These longitudinal attachments would normally dissociate in the absence of additional stabilizing factors, as shown in an in vitro reconstituted system where a high local concentration of α/β-tubulins bound to γTuRC was necessary to achieve nucleation25. In the native yeast system, however, the coiled-coil domains would stabilize the initial α/β-tubulin binding to the γTuRC. The alternating arrangement of the coiled-coil protein and recruited α/β-tubulin dimers present high-affinity gaps for additional α/β-tubulin dimers to fill, promoting intrinsically weak lateral α/β:α/β interactions and building up the first MT lattice row (Fig. 6, inset iii-a/b gray arrow, inset iv)49,50. Other than the Spc98NHD luminal bridge that links γTuSCs 3–5, together with Spc110CM1 (Fig. 4a,b), we observe no other factors that could laterally activate γTuRC into a closed state to match the MT lattice geometry. Moreover, γTuSC-1 in our native γTuRC structure, which has no α/β-tubulin bound to the first γ-tubulin (spoke 1), is in the open state and does not match the MT lattice geometry, indicating that MT lattice formation is necessary for γTuRC activation. These observations are consistent with the hypothesis that initial MT lattice formation during nucleation itself locks γTuRC into a closed state25. This ‘lattice-driven’ activation of γTuRC through lateral α/β-tubulin interactions was also proposed in recent studies of reconstituted human γTuRCs capping MT minus ends47,48. The initial α/β-tubulins at the γ-tubulin ring, positioned by the coiled-coil protein, would resemble tapered MT ends, which have been proposed to be a key structural intermediate of the growing MT tip5,24,51 (Fig. 6, inset iv). Overall, γTuRC, together with the coiled-coil protein recruiting/stabilizing α/β-tubulins, generates a stable scaffold that functions as a structural mimic of a native polymerizing MT plus end.
Spc110 recruits γTuSC modules to the SPB, where γTuSCs oligomerize into partial or complete γTuRCs assisted by the SPC110CM1 motif and the luminal bridge density. Before MT nucleation and lattice formation, γTuRC cycles between open and closed states (i). Top: the coiled-coil protein engages Spc98 and γ-tubulin (ii-a) and subsequently helps recruit and/or stabilize the first row of α/β-tubulin at the γTuRC (iii-a). Recruited and stabilized α/β-tubulin dimers extend longitudinally and fill up high-affinity gaps laterally (gray arrow iii-a), forming tapered MT ends suited for efficient MT polymerization and growth (iv). An alternative mechanism of nucleation is possible, shown at the bottom: α/β-tubulin dimers bind transiently to the γ-tubulin ring of open/closed yTuRC complexes (ii-b). The coiled-coil proteins bind to the extended Spc98: γ-tubulin: α-tubulin interfaces and stabilize the transient lateral interactions (iii-b). Recruited and stabilized α/β-tubulin dimers extend longitudinally and fill up high-affinity gaps laterally (gray arrow), forming tapered MT ends suited for efficient MT polymerization and growth (iv). In a third scenario (not depicted), the coiled coil binds to α/β-tubulin dimers before engaging γTuRC, as such stabilizing the weak lateral interactions. Given the molar excess of free α/β-tubulin in the yeast nucleus, this pathway is not likely to occur in vivo.
Methods
Preparation of the S. cerevisiae nuclei
S. cerevisiae NCYC74 nuclei were prepared as described in ref. 29. This yeast strain generates a high yield of SPBs.
SPB enrichment
SPBs were prepared from S. cerevisiae nuclei53 up to the sucrose equilibrium gradient step. For structural work, enriched SPBs were first dialyzed against bt-buffer (10 mM bis-Tris/HCl pH 6.5 and 0.1 mM MgCl2) supplemented with 20% dimethyl sulfoxide (DMSO) solution and then dialyzed once more against bt-buffer supplemented with 5% DMSO in a new dialysis cap. The final enriched SPBs were used for cryo-electron microscopy (cryo-EM) grid preparation and cryo-ET.
TUB4 mutagenesis and yeast strains
Mutations in TUB4 (termed tub4-401) were prepared by overlap extension PCR54. TUB4 constructs in a modified version of pRS 305K (ref. 55) containing a NotI site in the Kanamycin polylinker were cut with ApaLI and XhoI and integrated at LEU2 in a diploid strain containing a disruption of TUB4 with HIS3MX6. This strain, together with a wild type rescue, were sporulated and KAN+HIS+ spores retained.
Protein expression and purification
Spc98NHD (amino acids 1–95) was cloned into the pRsfDuet vector with an N-terminal 6xHis-tag. Spc98NHD was expressed in BL21 Star (DE3) cells using 2xTY medium. Cells were grown at 37 °C and induced using isopropyl β-d-1-thiogalactopyranoside when the OD600 reached 0.7–0.8, after which the temperature was lowered to 18 °C. The cell pellet was lysed by sonication in lysis buffer (50 mM Tris–HCl pH 8.0, 1 M NaCl, 10 mM imidazole and 5% glycerol) supplemented with benzamidine, EDTA-free protease inhibitor tablets, benzonase and lysozyme (Sigma-Aldrich). Clarified lysate was loaded onto HiTRAP TALON (GE Healthcare) columns, washed with ten column volumes and eluted using 300 mM imidazole in the lysis buffer. The 6xHis-tag was cleaved overnight using tobacco etch virus protease in a dialysis buffer containing 20 mM HEPES pH 7.8, 500 mM NaCl, 15 mM imidazole and 5% glycerol. Cleaved protein was reverse-passed through HiTRAP HP columns (Cytiva), and the flow-through protein was collected, concentrated to 50 mg ml−1 and stored.
Spc98NHD crystallization and structure determination
Purified Spc98NHD (50 mg ml−1) was crystallized using the Morpheus Screen (Molecular Dimensions) in a sitting drop format. One-hundred nanoliters of the Spc98NHD protein was mixed with 100 nl of the reservoir solution that contained 0.12 M monosaccharides, 0.1 M Buffer System 1 pH 6.5 and 37.5 % v/v of Precipitant Mix 4. The drop was left for 3 days at 18 °C when crystals were collected. No additional cryoprotectant was applied. The crystal was directly mounted on the LMB in-house X-ray generator (Rigaku FrR-E+ SuperBright), with data collected at a temperature of 100 K with wavelength of 1.54 Å. Data were indexed and integrated with MOSFLM56 and scaled and merged with AIMLESS57. Phasing was achieved by molecular replacement with Phaser58 as implemented in PHENIX, using a model generated by AlphaFold2 (ref. 59).
Spc98NHD structure refinement
The structure was refined using phenix.refine60. Ramachandran statistics were 95.74% preferred and 4.26% allowed.
Negative-stain electron microscopy
Three microliters of sample (SPBs or protein at 0.05 mg ml−1) were applied to glow-discharged (Edwards S150B glow discharger, 40 s) carbon supported 400 Cu grid (Agar Scientific) and allowed to adhere for 30 s. After that, uranyl acetate (UA) solution was applied to the sample, which was simultaneously blotted away using Whatmann blotting paper until 2 µl of UA solution was left on the grid. After 30 s of staining, the UA solution removed by blotting and the grid was left to dry.
SPB cryo-ET data collection
Three microliters of enriched SPBs mixed with bovine serum albumin-coated 10 nm gold fiducials (BBI Solutions) were applied to Lacey Carbon 300 mesh Au grids (Agar Scientific) either with a continuous carbon film or without any additional support, glow discharged using an Edwards S150B glow discharger (1 min for grids without additional carbon layer, or 30 s for grids with the additional carbon support layer), setting 6, 30–35 mA, 1.2 kV, 0.2 mBar (0.15 Torr). The grids were then flash frozen in liquid ethane using a ThermoFisherScientific Vitrobot IV (0.5 s waiting time, 2 s blotting time, −7 blotting force for grids without additional carbon support layer; 30 s waiting time, 5 s blotting time, 10 blotting force for grids with additional carbon support layer) using Whatman filter paper 1. Cryo-EM images were collected on a ThermoFisherScientific Titan Krios microscope operating at 300 keV using a Gatan K3 camera and a BioQuantum energy filter. Tilt series were collected using SerialEM at a nominal magnification of 81k, yielding a pixel size of 1.1 Å per pixel. The dose was set at 3.0 e− Å−2 per tilt image collected over ten frames. Data collection was performed using a dose-symmetric tilt-series acquisition scheme with 3° increments at stage tilts between −60 and +60, giving a total of 41 tilt-series images. The total dose over the tilt series was 123 e− Å−2.
γTuRC and MT data processing
A detailed pipeline for data processing is provided in Extended Data Fig. 1. All tilt series were preprocessed (motion correction and CTF estimation) in WARP61 and manually curated to exclude bad images from the tilt series. The dAutoalign4warp package was used to perform gold-fiducial-based tilt series alignments in dynamo62. Tomograms were generated in WARP at 8.8 Å per pixel and deconvolved for particle picking. Dynamo dtmslice was used to manually pick all γTuRC and MT particles63. γTuRC particles were picked as dipoles (z axis along the MT, away from the SPB) while MT particles were picked as filaments (z axis along the MT, away from the SPB). The coordinates (dynamo.tbl format) were converted to RELION 3.0 star files with the dynamo2m package. Subtomogram volumes and 3D CTF volumes were reconstructed at 6.6 Å per pixel for both the γTuRC and MT species using WARP. Initial 3D references were obtained in Dynamo by randomizing the azimuth of 1,000 γTuRC or MT particles and subsequently averaging them to obtain a featureless cone or barrel for the γTuRC and MT respectively. RELION 3.1 (refs. 64,65) was used to 3D to align the subtomogram volumes to γTuRC and MT 3D references. Three-dimensional classification in RELION did not reveal poor particles. The initial poses for γTuRC and the MTs were co-refined in M (ref. 66) at 3.3 Å per pixel. Tilt-series image warp, stage angles and defocus were also refined in M. γTuRC subtomogram volumes were reconstructed with M at 3.3 Å per pixel, and helical misalignments of the seam were resolved and corrected using RELION 3D classification and in-house scripts (fixalign), respectively. Napari subboxer67 was used to expand the γTuRC dataset by subboxing and refining γTuSC subunits, improving the resolution substantially.
Three-dimensional classification of MTs was used to separate MT species according to PF numbers, following the MiRP procedure68.
Model building
The atomic model of yeast γTuSC18 was fitted into a subboxed map of the γTuSC bound to two rows of α/β-tubulin and manually corrected in Coot69. The model for α/β-tubulin in the GDP state was fitted into the corresponding portion of the density and manually corrected. The coiled-coil density was built by positioning ideal α-helices into the coiled-coil density. The resulting model was subsequently refined with Phenix using standard settings. The model of the γTuSC was then fitted into one PF of the γTuRC, which was subsequently duplicated and repeated seven times to generate a complete model of the γTuRC, docking additional α/β-tubulin dimers into the map. The final model was further refined in PHENIX60 using standard settings.
CLMS
Enriched SPBs from the sucrose equilibrium gradient53 were dialyzed against 10 mM MES pH 6.5, 20% DMSO and 0.1 mM MgCl2. Dialyzed SPBs (500 µl) were made 17 mM in BS3 and left in ice for 20 min, then quenched by addition of 25 µl 1 M Tris pH 8.0 for 20 min in ice. Cross-linking efficiency was tested through western blot, showing that the Spc98 band shifts after cross-linking. The sample was precipitated by addition of three volumes of acetone and dried. The resulting precipitated proteins were then solubilized in a digestion buffer containing 8 M urea in 100 mM ammonium bicarbonate, aiming for an estimated protein concentration of 1 mg ml−1. Subsequently, the dissolved protein sample underwent reduction by the addition of 1 M dithiothreitol (DTT) to achieve a final concentration of 5 mM, followed by a 30 min incubation at 25 °C. The free sulfhydryl groups within the sample were alkylated by the addition of 500 mM iodoacetamide, resulting in a final concentration of 15 mM, and incubated at room temperature for 20 min in darkness. To neutralize any excess iodoacetamide, additional 1 M DTT was introduced, bringing the total concentration in the sample to 10 mM. The protein samples were then subjected to digestion with LysC at a 50:1 (m/m) protein to protease ratio, conducted at 25 °C for 6 h. Following this, the sample was diluted with 100 mM ammonium bicarbonate to achieve a urea concentration of 1.5 M, and trypsin was added at the same protein to protease ratio for further protein digestion overnight, approximately 15 h. The resulting peptides were desalted using C18 StageTips70.
For protein identification, 0.5% of the total peptides underwent direct analysis by liquid chromatography–tandem mass spectrometry (LC–MS/MS) (referred to as the protein ID sample). The LC–MS/MS analysis was performed using a Q Exactive HF mass spectrometer (Thermo Fisher Scientific) connected to an Ultimate 3000 RSLCnano system (Dionex, Thermo Fisher Scientific). Peptides were resuspended in a solution containing 1.6% v/v acetonitrile and 0.1% v/v formic acid, then injected onto a 50 cm EASY-Spray C18 LC column (Thermo Scientific) operating at 50 °C column temperature. The mobile phase consisted of water with 0.1% v/v formic acid (mobile phase A) and 80% v/v acetonitrile with 0.1% v/v formic acid (mobile phase B), with peptides loaded and separated at a flow rate of 0.25 μl min−1. A gradient was applied, starting with 2% to 9% B over 3 min, increasing to 30% B over 64 min, further to 44% B over 8 min and finally to 95% B over 5 min. Eluted peptides were ionized by an EASY-Spray source (Thermo Fisher Scientific) and introduced directly into the mass spectrometer, where MS data were acquired in data-dependent mode. The full scan mass spectrum was recorded with a resolution of 60,000. In each acquisition cycle, the ten most intense ions with a charge state from 2+ to 6+ were isolated and fragmented using higher-energy collisional dissociation with a normalized collision energy of 27%. The fragmentation spectra were recorded with a resolution of 50,000. Dynamic exclusion was enabled with a single repeat count and 30 s exclusion duration.
The remaining peptides were subjected to fractionation using size exclusion chromatography to enrich for cross-linked peptides71. Peptides were separated using a Superdex 30 Increase 3.2/300 column (GE Healthcare) at a flow rate of 10 μl min−1. The earliest six peptide-containing fractions (fractions 6–11, 50 μl each) were collected, solvent removed using a vacuum concentrator, and then analyzed by LC–MS/MS. LC–MS/MS analysis of the size exclusion chromatography (SEC) fractions was performed using an Orbitrap Astral Mass Spectrometer (Thermo Fisher Scientific) connected to a Vanquish Neo UHPLC System (Thermo Fisher Scientific). Each SEC fraction was resuspended in a solution containing 1.6% v/v acetonitrile and 0.1% v/v formic acid. Peptides were injected onto a 5.5 cm High Throughput μPAC Neo HPLC Column (Thermo Scientific) operating at 50 °C column temperature. The mobile phase consisted of water with 0.1% v/v formic acid (mobile phase A) and 80% v/v acetonitrile with 0.1% v/v formic acid (mobile phase B), with peptides separated at a flow rate of 0.3 μl min−1. The separation gradient was optimized for each SEC fraction, ranging from 2% to 45–55% B over 88 min, followed by a ramp-up to 95% B within 7 min. Eluted peptides were ionized by an EASY-Spray source (Thermo Fisher Scientific) and introduced directly into the mass spectrometer.
For each SEC fraction, three LC–MS/MS analyses were conducted: the first without high field asymmetric waveform ion mobility spectrometry (FAIMS) applied, the second with FAIMS applied with a single compensation voltage (CV) filter and the third with FAIMS applied with two interval CV filters. The CV voltage applied for each SEC fraction was determined on the basis of previous optimization for complex peptide mixtures72.
The MS data were acquired in the data-dependent mode with the top-speed option. For each 2.5 s acquisition cycle, the full scan mass spectrum was recorded in the Orbitrap with a resolution of 120,000. The ions with a charge state from 3+ to 7+ were isolated and fragmented using higher-energy collisional dissociation with a normalized collision energy of 30%. The fragmentation spectra were then recorded in the Astral mass analyzer. Dynamic exclusion was enabled with single repeat count and 60 s exclusion duration.
Protein identification was carried out using MaxQuant (version 2.4.10.0), searching against the S. cerevisiae protein sequence database downloaded from UniProt, with default search parameters and intensity-based absolute quantification (iBAQ) enabled.
For SEC fractions, MS2 peak lists were generated using the MSConvert module in ProteoWizard (version 3.0.11729), and identification of cross-linked peptides was performed using xiSEARCH software (version 1.7.6.4) against the sequences and reversed sequences of 315 proteins, including SPB subunits and co-isolated proteins identified with an intensity-based absolute quantification over 1 × 106 in the protein ID sample. The following parameters were applied for the search:
MS accuracy = 3 ppm; MS2 accuracy = 5 ppm
enzyme = trypsin (with full tryptic specificity);
allowed number of missed cleavages = 3
missing monoisotopic peak = 2
cross-linker = BS3 (the reaction specificity for BS3 was assumed to be for lysine, serine, threonine, tyrosine and protein N termini)
fixed modifications = carbamidomethylation on cysteine
variable modifications = oxidation on methionine
BS3 loop link
hydrolyzed BS3 on one end
Tris-reacted BS3 on one end
maximum variable modification per peptide = 2
Cross-linked peptide candidates with a minimum of three matched fragment ions (with at least two containing a cross-linked residue) in each cross-linked peptide were filtered using xiFDR73. A false discovery rate of 10% at the residue-pair level was applied, with the ‘boost between’ option enabled for heteromeric cross-links.
MS data have been deposited in jPOST74.
Proteomics analysis of enriched SPBs
Twenty gel bands were cut out of a sodium dodecyl sulfate gel of the enriched SPBs. These gel samples were destained with 50% v/v acetonitrile and 50 mM ammonium bicarbonate, reduced with 10 mM DTT and alkylated with 55 mM iodoacetamide. Digestion was with 6 ng μl−1 trypsin (Promega) overnight at 37 °C, and peptides were extracted in 2% v/v formic acid 2% v/v acetonitrile and analyzed by nano-scale capillary LC–MS/MS using an Ultimate U3000 HPLC (Thermo Fisher Scientific Dionex) to deliver a flow of approximately 300 nl min−1. A µ-precolumn cartridge C18 Acclaim PepMap 100 (5 µm, 300 µm × 5 mm (Thermo Fisher Scientific Dionex) trapped the peptides before separation on a C18 Acclaim PepMap100 3 µm, 75 µm × 250 mm (Thermo Fisher Scientific Dionex). Peptides were eluted with a 70 min gradient of acetonitrile (5% to 40%). The analytical column outlet was directly interfaced via a modified nano-flow electrospray ionization source, with a hybrid linear quadrupole ion trap mass spectrometer (Orbitrap QExactive, ThermoScientific). Data-dependent analysis was carried out, using a resolution of 60,000 for the full MS spectrum, followed by ten MS/MS spectra in the linear ion trap. MS spectra were collected over a m/z range of 200–1,800. MS/MS scans were collected using threshold energy of 35 for collision-induced dissociation. LC–MS/MS data were then searched against the UNIPROT database, using the Mascot search engine program (Matrix Science). Database search parameters were set with a precursor tolerance of 10 ppm and a fragment ion mass tolerance of 0.8 Da. One missed enzyme cleavage was allowed, and variable modifications for oxidized methionine, carbamidomethyl and phosphorylated serine, threonine and tyrosine were included.
The MS proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE75 partner repository with the dataset identifier PXD050372.
Data visualization
All data were visualized and figures were prepared using ChimeraX76. The video was prepared using ArtiaX77. CLMS data were visualized and the figure was prepared using xiVIEW78,79.
Reporting summary
Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article.
Data availability
All data are available in the main text, Methods, Supplementary information and Extended data. Atomic coordinates and cryo-EM density maps of the γTuRC capping MTs (PDBs: 8QV2, 8QV3; maps: EMD-18665, EMD-18666) and MT lattice (PDB: 8QV0; map: EMD-18664) have been deposited in the Protein Data Bank (www.rcsb.org) and the Electron Microscopy Data Bank (https://ebi.ac.uk/pdbe/emdb/), respectively, and are also listed in Table 1. The atomic coordinates and crystallographic data for Spc98NHD have been deposited in the Protein Data Bank (www.rcsb.org) (accession code 8QRY) and are listed in Table 2. CLMS data have been deposited in jPOST (https://repository.jpostdb.org) accession code JPST002974 (PRIDE (https://www.ebi.ac.uk/pride) dataset identifier PXD050440). The MS proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD050372. Source data are provided with this paper.
Code availability
Source code for the Napari subboxer is available from GitHub at https://github.com/alisterburt/napari-subboxer.git (ref. 67).
References
Akhmanova, A. & Steinmetz, M. O. Control of microtubule organization and dynamics: two ends in the limelight. Nat. Rev. Mol. Cell Biol. 16, 711–726 (2015).
Brouhard, G. J. & Rice, L. M. Microtubule dynamics: an interplay of biochemistry and mechanics. Nat. Rev. Mol. Cell Biol. 19, 451–463 (2018).
Voter, W. A. & Erickson, H. P. The kinetics of microtubule assembly. Evidence for a two-stage nucleation mechanism. J. Biol. Chem. 259, 10430–10438 (1984).
Kuchnir Fygenson, D., Flyvbjerg, H., Sneppen, K., Libchaber, A. & Leibler, S. Spontaneous nucleation of microtubules. Phys. Rev. E 51, 5058–5063 (1995).
Roostalu, J. & Surrey, T. Microtubule nucleation: beyond the template. Nat. Rev. Mol. Cell Biol. 18, 702–710 (2017).
Zheng, Y., Wong, M. L., Alberts, B. & Mitchison, T. Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex. Nature 378, 578–583 (1995).
Thawani, A. & Petry, S. Molecular insight into how gamma-TuRC makes microtubules. J. Cell Sci. https://doi.org/10.1242/jcs.245464 (2021).
Liu, P., Wurtz, M., Zupa, E., Pfeffer, S. & Schiebel, E. Microtubule nucleation: the waltz between gamma-tubulin ring complex and associated proteins. Curr. Opin. Cell Biol. 68, 124–131 (2021).
Paz, J. & Luders, J. Microtubule-organizing centers: towards a minimal parts list. Trends Cell Biol. 28, 176–187 (2018).
Liu, P. et al. Insights into the assembly and activation of the microtubule nucleator gamma-TuRC. Nature 578, 467–471 (2020).
Wieczorek, M. et al. Asymmetric molecular architecture of the human gamma-tubulin ring complex. Cell 180, 165–175 e116 (2020).
Consolati, T. et al. Microtubule nucleation properties of single human gammaTuRCs explained by their cryo-EM structure. Dev. Cell 53, 603–617 e608 (2020).
Wieczorek, M. et al. Biochemical reconstitutions reveal principles of human gamma-TuRC assembly and function. J. Cell Biol. https://doi.org/10.1083/jcb.202009146 (2021).
Wurtz, M. et al. Reconstitution of the recombinant human gamma-tubulin ring complex. Open Biol. 11, 200325 (2021).
Zimmermann, F. et al. Assembly of the asymmetric human gamma-tubulin ring complex by RUVBL1-RUVBL2 AAA ATPase. Sci. Adv. 6, 1–20 (2020).
Kollman, J. M., Polka, J. K., Zelter, A., Davis, T. N. & Agard, D. A. Microtubule nucleating gamma-TuSC assembles structures with 13-fold microtubule-like symmetry. Nature 466, 879–882 (2010).
Kollman, J. M. et al. Ring closure activates yeast gammaTuRC for species-specific microtubule nucleation. Nat. Struct. Mol. Biol. 22, 132–137 (2015).
Brilot, A. F. et al. CM1-driven assembly and activation of yeast gamma-tubulin small complex underlies microtubule nucleation. eLife 10, 1–35 (2021).
Zupa, E. et al. The cryo-EM structure of a gamma-TuSC elucidates architecture and regulation of minimal microtubule nucleation systems. Nat. Commun. 11, 5705 (2020).
Lyon, A. S. et al. Higher-order oligomerization of Spc110p drives gamma-tubulin ring complex assembly. Mol. Biol. Cell 27, 2245–2258 (2016).
Lin, T. C. et al. Cell-cycle dependent phosphorylation of yeast pericentrin regulates gamma-TuSC-mediated microtubule nucleation. eLife 3, e02208 (2014).
Leong, S. L. et al. Reconstitution of microtubule nucleation in vitro reveals novel roles for Mzt1. Curr. Biol. 29, 2199–2207 e2110 (2019).
Knop, M. & Schiebel, E. Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation. EMBO J. 17, 3952–3967 (1998).
Wieczorek, M., Bechstedt, S., Chaaban, S. & Brouhard, G. J. Microtubule-associated proteins control the kinetics of microtubule nucleation. Nat. Cell Biol. 17, 907–916 (2015).
Thawani, A. et al. The transition state and regulation of gamma-TuRC-mediated microtubule nucleation revealed by single molecule microscopy. eLife 9, 1–34 (2020).
Thawani, A., Kadzik, R. S. & Petry, S. XMAP215 is a microtubule nucleation factor that functions synergistically with the gamma-tubulin ring complex. Nat. Cell Biol. 20, 575–585 (2018).
Ali, A., Vineethakumari, C., Lacasa, C. & Luders, J. Microtubule nucleation and gammaTuRC centrosome localization in interphase cells require ch-TOG. Nat. Commun. 14, 289 (2023).
Bullitt, E., Rout, M. P., Kilmartin, J. V. & Akey, C. W. The yeast spindle pole body is assembled around a central crystal of Spc42p. Cell 89, 1077–1086 (1997).
Rout, M. P. & Kilmartin, J. V. Components of the yeast spindle and spindle pole body. J. Cell Biol. 111, 1913–1927 (1990).
Winey, M. et al. Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle. J. Cell Biol. 129, 1601–1615 (1995).
Zupa, E., Liu, P., Wurtz, M., Schiebel, E. & Pfeffer, S. The structure of the gamma-TuRC: a 25-years-old molecular puzzle. Curr. Opin. Struct. Biol. 66, 15–21 (2021).
Howes, S. C. et al. Structural differences between yeast and mammalian microtubules revealed by cryo-EM. J. Cell Biol. 216, 2669–2677 (2017).
Khodjakov, A. & Rieder, C. L. The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules. J. Cell Biol. 146, 585–596 (1999).
Aldaz, H., Rice, L. M., Stearns, T. & Agard, D. A. Insights into microtubule nucleation from the crystal structure of human gamma-tubulin. Nature 435, 523–527 (2005).
Kilmartin, J. V. Purification of yeast tubulin by self-assembly in vitro. Biochemistry 20, 3629–3633 (1981).
Zahm, J. A., Stewart, M. G., Carrier, J. S., Harrison, S. C. & Miller, M. P. Structural basis of Stu2 recruitment to yeast kinetochores. eLife https://doi.org/10.7554/eLife.65389 (2021).
Wang, P. J. & Huffaker, T. C. Stu2p: a microtubule-binding protein that is an essential component of the yeast spindle pole body. J. Cell Biol. 139, 1271–1280 (1997).
Haase, K. P. et al. Stu2 uses a 15-nm parallel coiled coil for kinetochore localization and concomitant regulation of the mitotic spindle. Mol. Biol. Cell 29, 285–294 (2018).
Ayaz, P. et al. A tethered delivery mechanism explains the catalytic action of a microtubule polymerase. eLife 3, e03069 (2014).
Ayaz, P., Ye, X., Huddleston, P., Brautigam, C. A. & Rice, L. M. A. TOG:alphabeta-tubulin complex structure reveals conformation-based mechanisms for a microtubule polymerase. Science 337, 857–860 (2012).
Wieczorek, M., Huang, T. L., Urnavicius, L., Hsia, K. C. & Kapoor, T. M. MZT proteins form multi-faceted structural modules in the gamma-tubulin ring complex. Cell Rep. 31, 107791 (2020).
Huang, T. L., Wang, H. J., Chang, Y. C., Wang, S. W. & Hsia, K. C. Promiscuous binding of microprotein Mozart1 to gamma-tubulin complex mediates specific subcellular targeting to control microtubule array formation. Cell Rep. 31, 107836 (2020).
Cota, R. R. et al. MZT1 regulates microtubule nucleation by linking gammaTuRC assembly to adapter-mediated targeting and activation. J. Cell Sci. 130, 406–419 (2017).
Akram, Z. et al. Yeast as a model to understand actin-mediated cellular functions in mammals-illustrated with four actin cytoskeleton proteins. Cells https://doi.org/10.3390/cells9030672 (2020).
Lin, T. C. et al. MOZART1 and gamma-tubulin complex receptors are both required to turn gamma-TuSC into an active microtubule nucleation template. J. Cell Biol. 215, 823–840 (2016).
Moritz, M., Braunfeld, M. B., Sedat, J. W., Alberts, B. & Agard, D. A. Microtubule nucleation by gamma-tubulin-containing rings in the centrosome. Nature 378, 638–640 (1995).
Aher, A., Urnavicius, L., Xue, A., Neselu, K. & Kapoor, T. M. Structure of the γ-tubulin ring complex-capped microtubule. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-024-01264-z
Brito, C., Serna, M., Guerra, P., Llorca, O. & Surrey, T. Transition of human gamma-tubulin ring complex into a closed conformation during microtubule nucleation. Science https://doi.org/10.1126/science.adk6160 (2024).
Nogales, E. & Zhang, R. Visualizing microtubule structural transitions and interactions with associated proteins. Curr. Opin. Struct. Biol. 37, 90–96 (2016).
Manka, S. W. & Moores, C. A. The role of tubulin–tubulin lattice contacts in the mechanism of microtubule dynamic instability. Nat. Struct. Mol. Biol. 25, 607–615 (2018).
Mickolajczyk, K. J., Geyer, E. A., Kim, T., Rice, L. M. & Hancock, W. O. Direct observation of individual tubulin dimers binding to growing microtubules. Proc. Natl Acad. Sci. USA 116, 7314–7322 (2019).
Forsberg, B. O., Shah, P. N. M. & Burt, A. A robust normalized local filter to estimate compositional heterogeneity directly from cryo-EM maps. Nat. Commun. 14, 5802 (2023).
Wigge, P. A. et al. Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J. Cell Biol. 141, 967–977 (1998).
Higuchi, R., Krummel, B. & Saiki, R. K. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res. 16, 7351–7367 (1988).
Taxis, C. & Knop, M. System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae. Biotechniques 40, 73–78 (2006).
Battye, T. G., Kontogiannis, L., Johnson, O., Powell, H. R. & Leslie, A. G. iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM. Acta Crystallogr. D 67, 271–281 (2011).
Evans, P. R. & Murshudov, G. N. How good are my data and what is the resolution? Acta Crystallogr. D 69, 1204–1214 (2013).
McCoy, A. J. et al. Phaser crystallographic software. J. Appl. Crystallogr. 40, 658–674 (2007).
Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. Nature 596, 583–589 (2021).
Afonine, P. V. et al. Towards automated crystallographic structure refinement with phenix.refine. Acta Crystallogr. D 68, 352–367 (2012).
Tegunov, D. & Cramer, P. Real-time cryo-electron microscopy data preprocessing with Warp. Nat. Methods 16, 1146–1152 (2019).
Burt, A., Gaifas, L., Dendooven, T. & Gutsche, I. A flexible framework for multi-particle refinement in cryo-electron tomography. PLoS Biol. 19, e3001319 (2021).
Castano-Diez, D., Kudryashev, M., Arheit, M. & Stahlberg, H. Dynamo: a flexible, user-friendly development tool for subtomogram averaging of cryo-EM data in high-performance computing environments. J. Struct. Biol. 178, 139–151 (2012).
Bharat, T. A. & Scheres, S. H. Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION. Nat. Protoc. 11, 2054–2065 (2016).
Scheres, S. H. RELION: implementation of a Bayesian approach to cryo-EM structure determination. J. Struct. Biol. 180, 519–530 (2012).
Tegunov, D., Xue, L., Dienemann, C., Cramer, P. & Mahamid, J. Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5A in cells. Nat. Methods 18, 186–193 (2021).
napari-subboxer. GitHub https://github.com/alisterburt/napari-subboxer.git (2021).
Cook, A. D., Manka, S. W., Wang, S., Moores, C. A. & Atherton, J. A microtubule RELION-based pipeline for cryo-EM image processing. J. Struct. Biol. 209, 107402 (2020).
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D 66, 486–501 (2010).
Rappsilber, J., Mann, M. & Ishihama, Y. Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Nat. Protoc. 2, 1896–1906 (2007).
Leitner, A., Walzthoeni, T. & Aebersold, R. Lysine-specific chemical cross-linking of protein complexes and identification of cross-linking sites using LC-MS/MS and the xQuest/xProphet software pipeline. Nat. Protoc. 9, 120–137 (2014).
Sinn, L. R., Giese, S. H., Stuiver, M. & Rappsilber, J. Leveraging parameter dependencies in high-field asymmetric waveform ion-mobility spectrometry and size exclusion chromatography for proteome-wide cross-linking mass spectrometry. Anal. Chem. 94, 4627–4634 (2022).
Muller, F., Fischer, L., Chen, Z. A., Auchynnikava, T. & Rappsilber, J. On the reproducibility of label-free quantitative cross-linking/mass spectrometry. J. Am. Soc. Mass Spectrom. 29, 405–412 (2018).
Okuda, S. et al. jPOSTrepo: an international standard data repository for proteomes. Nucleic Acids Res. 45, D1107–D1111 (2017).
Perez-Riverol, Y. et al. The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences. Nucleic Acids Res. 50, D543–D552 (2022).
Meng, E. C. et al. UCSF ChimeraX: Tools for structure building and analysis. Protein Sci. https://doi.org/10.1002/pro.4792 (2023).
Ermel, U. H., Arghittu, S. M. & Frangakis, A. S. ArtiaX: an electron tomography toolbox for the interactive handling of sub-tomograms in UCSF ChimeraX. Protein Sci. 31, e4472 (2022).
Combe, C. W., Fischer, L. & Rappsilber, J. xiNET: cross-link network maps with residue resolution. Mol. Cell Proteom. 14, 1137–1147 (2015).
Graham, M., Combe, C., Kolbowski, L. & Rappsilber, J. xiView: a common platform for the downstream analysis of crosslinking mass spectrometry data. Preprint at bioRxiv https://doi.org/10.1101/561829 (2019).
Acknowledgements
We are grateful to the LMB EM Facility for help with the EM data collection, J. Grimmett and T. Darling for computing, and T. Morgan and F. Begum of the Mass Spec Facility for proteomic analysis. We also thank members of Barford group for their input and useful discussions. J.V.K. is an Emeritus Staff Member of the MRC-LMB and is very grateful to S. Munro for providing a bench and facilities in his group. For the purpose of open access, the author has applied a CC BY public copyright license to any author accepted manuscript version arising. Grant funding was provided by UKRI/Medical Research Council MC_UP_1201/6 (Da.B.), Cancer Research UK C576/A14109 (Da.B.), Boehringer Ingleheim Fonds Fellowship (S.Y.) and Wellcome Trust [227434] (J.R.). The Wellcome Centre for Cell Biology is supported by core funding from the Wellcome Trust [203149] (J.R.). Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2008 – 390540038 – UniSysCat. Si-M/‘Der Simulierte Mensch’, a Science Framework of Technische Universität Berlin and Charité – Universitätsmedizin Berlin, Berlin, Germany, provided access to the mass spectrometer system (J.R.).
Author information
Authors and Affiliations
Contributions
Da.B., S.Y., T.D. and J.V.K. conceived the study. J.V.K., T.D. and S.Y. enriched the SPBs. T.D. and S.Y. prepared EM grids, collected and processed EM data, with help from A.B., and built the models. S.Y. purified and crystallized Spc98NHD, and Do.B. collected and processed crystallography data. J.V.K. performed yeast genetic experiments. T.D. and J.V.K. cross-linked the enriched SPBs and Z.A.C. and J.R. performed the CLMS experiments on SPBs. S.Y., T.D., Da.B., J.V.K., Z.A.C. and J.R. analyzed the data and prepared the manuscript with input from all authors.
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing interests.
Peer review
Peer review information
Nature Structural & Molecular Biology thanks Michael Cianfrocco and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Primary Handling Editor: Katarzyna Ciazynska, in collaboration with the Nature Structural & Molecular Biology team. Peer reviewer reports are available.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Extended data
Extended Data Fig. 1 Cryo-ET data processing scheme.
a, (left) Representative electron micrograph at 13,000x magnification of the enriched SPB sample on lacey grids. Similar SPB structures were observed in more than 500 independent tomograms. (right) Slice through a tomogram with red circles indicating centre of the dipole geometry used to pick γTuRCs, the arrows define the z-axis. Blue circles indicate positions along which microtubules were picked as a filament, the z-axis is pointed away from the SPB. Representative of more than 500 tomograms. b, Custom data processing pipeline, also outlined in the Methods section. Tilt-series (TS) were preprocessed in Warp and aligned using dautoalign4warp (Dynamo). Bin8 tomograms were generated in Warp and particle picking was performed in Dynamo, with γTuRC picked as dipoles and MTs picked as filaments. Sub-tomogram volumes were extracted in Warp and refined using RELION. Both γTuRC and MT particles were co-refined in M to give a final consensus refinement map. To identify and correct particle misalignments, a manual procedure (the Fixalign procedure) was developed: γTuRC particles were classified without alignment to identify classes where the seam of the γTuRC 3D class (full black line) was misaligned with respect to the seam of the initial γTuRC reference (dotted line). Misalignment was corrected by manually realigning 3D class averages in ChimeraX and applying the corresponding transformations to the respective particle stacks of the misaligned 3D class averages (arrow from full black line to dotted line). The Fixalign procedure improved density of γTuSCs-1 and γTuSCs-7 substantially (see also panel d). Next, A custom-built Napari sub-boxer plugin was used to sub-box and mask γTuRC particles into individual γTuSCs with two rows of α/β-tubulin to expand the dataset and improve the resolution. Sub-boxed γTuSCs were subjected to 3D refinement in RELION 3.1. WBP: weighted back projection. c, Fourier-shell correlation (FSC) curves for the reconstructions used in this study. d, Bottom view of the γTuRC before and after manually correcting misalignment of the γTuRC helical register (Fixalign). Prior to manual realignment the density for γTuSC-1 and γTuSC-7 was diffuse, but improved substantially after realignment and further 3D refinements.
Extended Data Fig. 2 γTuRC geometry and interactions during MT nucleation.
a, Top-down view of γTuRC models with numbered γTuSCs and γ-tubulins (left). A molecular model of the γTuSC module with bound Spc110 protein and annotated GRIP1 and GRIP2 domains, is depicted on the right. b, Molecular model of the γTuRC seam shows that the first γ-tubulin bound to Spc97 is positioned precisely below the last Spc98 bound to the last (14th) γ-tubulin, with a large gap between them. This blocks α/β-tubulin binding to spoke 1 of γTuRC. c, Cryo-ET map of a sub-boxed γTuSC bound to the first two rows of α/β-tubulin, displaying the lateral α:α-tubulin (double contact), β:β-tubulin (double contact) and γ:γ-tubulin interfaces (single contact).
Extended Data Fig. 3 Details of Spc110 and coiled-coil interactions with the γTuRC.
a, Consensus cryo-ET map of the γTuRC with a fitted molecular model of the γTuRC shows that the secondary structure and path of Spc110NCC and Spc110CM1 are resolved and in agreement with previous reconstituted structures18. b, At lower threshold the sub-tomogram average shows that Spc110NCC densities continue downwards until they converge on a single large stalk density. The map was blurred with a B-factor of 400 for visualization purposes. c, 3D classification of MTs was used to separate MT species according to protofilament numbers, following the MiRP procedure68. Representative slices through reference volumes used during 3D classification of the microtubule sub-tomogram volumes. 97% of spindle microtubules contain 13 protofilaments.
Extended Data Fig. 4 Spc98 presents a unique interface to bind the coiled-coil density.
a, Electrostatic surface potential of Spc98 and Spc97 in the γTuSC structure shows that the coiled-coil protein is lined against a positively charged patch on Spc98GRIP2. b, Alphafold2 predictions and cryo-EM density insets of the interface between the γ-tubulinCTR and Spc98GRIP2 (top panel) and Spc97GRIP2 (lower panel). The γ-tubulinCTR is predicted to bind extensively along the Spc98GRIP2 domain and extends beyond the conserved ‘DSYL’ motif towards the coiled-coil density. At the Spc97GRIP2 domain on the other hand, the γ-tubulinCTR tail is not predicted to interact beyond the ‘DSYL’ motif. c, γ-tubulin pLDDT scores mapped on the Alphafold2 predictions shown in panel b. γ-tubulin is coloured by the pLDDT score while Spc98 and Spc97 are coloured by subunit. PAE plots for the best model are also shown as insets. d, The unstructured γ-tubulinCTR extends toward the coiled-coil density in the γTuRC average.
Extended Data Fig. 5 Mass spectrometry analysis of the components present in the SPB enrichment sample.
a, Mass spectrometry analysis of the enriched SPB sample, displaying only identified proteins with known function in microtubule−related processes. The full list of identified proteins is provided in the Supplementary Data 1 spreadsheet. b, Map of intermolecular cross-links between Stu2 and γTuRC components obtained through CL-MS analysis of the enriched SPB preparation. The Stu2 N-terminus crosslinks to the Spc110 N-terminus at γTuRC. Other proteins that were crosslinked to STU2 were shown as nodes.
Extended Data Fig. 6 Structural comparison and analysis of the Spc98NHD.
a, Structures of the N-terminal Helical Domains (NHD) of Spc98 and homologs, without (left) and with (right) Mzt1 bound. The S. cerevisiae genome does not contain the mzt1 gene. Structurally comparable helices are annotated with A, B and C to highlight similarities between Spc98NHD and functionally equivalent proteins in other organisms. A direct overlay between the ABC helices of Spc98NHD and GCP3NHD is shown in the middle. GCP3NHD and GCP6NHD have very similar folds. b, Alphafold2 pLDDT PAE scores of the best trimeric Spc98NHD prediction. c, Bottom view of the γTuRC map shows bridging connections between Spc98 modules and the luminal density.
Supplementary information
Supplementary Video 1
Cryo-electron tomograph of enriched budding yeast SPB with atomic modes of γTuRC and MTs built into cryo-ET densities.
Supplementary Data 1
Proteomics data listing proteins identified in the enriched budding yeast SPB sample.
Source data
Source Data Extended Data Fig. 5
Raw data for total peptide counts for proteins shown in Extended Data Fig. 5a.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Dendooven, T., Yatskevich, S., Burt, A. et al. Structure of the native γ-tubulin ring complex capping spindle microtubules. Nat Struct Mol Biol 31, 1134–1144 (2024). https://doi.org/10.1038/s41594-024-01281-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41594-024-01281-y
- Springer Nature America, Inc.
This article is cited by
-
Cryo-EM structures of γ-TuRC reveal molecular insights into microtubule nucleation
Nature Structural & Molecular Biology (2024)