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PEPTIDE LIBRARIES

Affinity selection and sequencing

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Powerful combinatorial peptide library methods allow the discovery of peptide leads from diverse libraries. A new platform based on tandem mass spectrometry peptide sequencing coupled with high-performance size-exclusion chromatography enables identification of high-affinity peptidic ligands from focused libraries.

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Fig. 1: Affinity selection–mass spectrometry (AS–MS) method for identifying high-affinity peptide ligands.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Medicine, Division of Hematology & Oncology, School of Medicine, UC Davis NCI-designated Comprehensive Cancer Center, University of California Davis, Sacramento, CA, USA

    Kit S. Lam

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  1. Kit S. Lam
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Correspondence to Kit S. Lam.

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Lam, K.S. Affinity selection and sequencing. Nat Chem Biol 15, 320–321 (2019). https://doi.org/10.1038/s41589-019-0253-2

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