An improved light-sheet microscope images live cells at sub-100-nm axial resolution.
References
Cao, B., Wang, G., Li, J. & Pertsinidis, A. Nat. Biotechnol. https://doi.org/10.1038/s41587-021-01042-y (2021).
Hell, S. W., Stelzer, E. H. K., Lindek, S. & Cremer, C. Opt. Lett. 19, 222–224 (1994).
Gustafsson, M. G., Agard, D. A. & Sedat, J. W. J. Microsc. 195, 10–16 (1999).
Shtengel, G. et al. Proc. Natl Acad. Sci. USA 106, 3125–3130 (2009).
Chen, B.-C. et al. Science 346, 1257998 (2014).
Chang, B.-J., Dean, K. M. & Fiolka, R. Opt. Express 28, 27052–27077 (2020).
Hell, S. W., Lindek, S. & Stelzer, E. H. K. J. Mod. Opt. 41, 675–681 (1994).
Shao, L. et al. Biophys. J. 94, 4971–4983 (2008).
Acknowledgements
The author is grateful for funding by the National Institutes of Health (R35GM133522) and thanks James Manton for help simulating the 4Pi point spread function used in Fig. 1b,d.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The author declares no competing interests.
Rights and permissions
About this article
Cite this article
Fiolka, R. Light-sheet microscopy at high resolution. Nat Biotechnol 39, 1345–1346 (2021). https://doi.org/10.1038/s41587-021-01101-4
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41587-021-01101-4
- Springer Nature America, Inc.
This article is cited by
-
Unraveling the Neural Circuits: Techniques, Opportunities and Challenges in Epilepsy Research
Cellular and Molecular Neurobiology (2024)