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Engineering human knock-in organoids

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The lack of endogenous reporter lines is a bottleneck in the study of subcellular dynamics in human adult stem cell (ASC)-derived organoids. An approach using CRISPR–Cas9-mediated homology-independent organoid transgenesis (CRISPR–HOT) in ASC-derived organoids now narrows the gap between basic research and translational studies in human organoids.

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Fig. 1: Workflow and cellular applications of CRISPR–HOT in ASC-derived organoids.

References

  1. Lancaster, M. A. & Huch, M. Dis. Model. Mech. 12, dmm039347 (2019).

    Article  CAS  Google Scholar 

  2. McCauley, H. A. & Wells, J. M. Development 144, 958–962 (2017).

    Article  CAS  Google Scholar 

  3. Liu, C., Oikonomopoulos, A., Sayed, N. & Wu, J. C. Development 145, dev156166 (2018).

    Article  Google Scholar 

  4. Fiorotto, R. et al. Biochim. Biophys. Acta. Mol. Basis Dis. 1865, 920–928 (2019).

    Article  CAS  Google Scholar 

  5. Wang, M., Zhang, L. & Gage, F. H. Protein Cell 11, 45–59 (2020).

    Article  Google Scholar 

  6. Nie, J. & Hashino, E. EMBO Rep. 18, 367–376 (2017).

    Article  CAS  Google Scholar 

  7. Sugimoto, S. et al. Cell Stem Cell 22, 171–176.e5 (2018).

    Article  CAS  Google Scholar 

  8. Artegiani, B. et al. Nat. Cell. Biol. https://doi.org/10.1038/s41556-020-0472-5 (2020).

  9. Cortina, C. et al. EMBO Mol. Med. 9, 869–879 (2017).

    Article  CAS  Google Scholar 

  10. Beumer, J. et al. Nat. Cell Biol. 20, 909–916 (2018).

    Article  CAS  Google Scholar 

  11. Serra, D. et al. Nature 569, 66–72 (2019).

    Article  CAS  Google Scholar 

  12. Verissimo, C. S. et al. eLife 5, e18489 (2016).

    Article  Google Scholar 

  13. Sakaue-Sawano, A. et al. Cell 132, 487–498 (2008).

    Article  CAS  Google Scholar 

  14. Dutta, D., Heo, I. & O’Connor, R. J. Vis. Exp. 14, 151 (2019).

    Google Scholar 

  15. Bar-Ephraim, Y.E., Kretzschmar, K. & Clevers, H. Nat. Rev. Immunol. https://doi.org/10.1038/s41577-019-0248-y (2019).

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Author notes

  1. These authors contributed equally: Qiutan Yang, Koen C. Oost.

Authors and Affiliations

  1. Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland

    Qiutan Yang, Koen C. Oost & Prisca Liberali

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  1. Qiutan Yang
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  2. Koen C. Oost
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  3. Prisca Liberali
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Correspondence to Prisca Liberali.

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Yang, Q., Oost, K.C. & Liberali, P. Engineering human knock-in organoids. Nat Cell Biol 22, 261–263 (2020). https://doi.org/10.1038/s41556-020-0478-z

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