Abstract
The basal cell compartment in many epithelial tissues is generally believed to serve as an important pool of stem cells. However, basal cells are heterogenous and the stem cell subpopulation within basal cells is not well elucidated. Here we uncover that the core epithelial-to-mesenchymal transition (EMT) inducer Zeb1 is expressed in a prostate basal cell subpopulation. The Zeb1+ prostate epithelial cells are multipotent prostate basal stem cells (PBSCs) that can self-renew and generate functional prostatic glandular structures at the single-cell level. Genetic ablation studies reveal an indispensable role for Zeb1 in prostate basal cell development. Utilizing unbiased single-cell transcriptomic analysis of over 9000 mouse prostate basal cells, we confirm the existence of the Zeb1+ basal cell subset. Moreover, Zeb1+ epithelial cells can be detected in mouse and human prostate tumors. Identification of the PBSC and its transcriptome profile is crucial to advance our understanding of prostate development and tumorigenesis.
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Introduction
The prostatic epithelium, comprised of basal cells, secretory luminal cells and rare neuroendocrine cells, can serve as an excellent model to study stem cell biology due to its ability to regress and regenerate after repeated rounds of androgen deprivation and restoration in mice and rats1,2. Using in vivo sub-renal capsule tissue regeneration, cell division mode analyses or in vitro organoid-forming assays, we and others have demonstrated the existence of prostate stem/progenitor cells in both basal and luminal prostate epithelia3,4,5,6,7,8,9,10. Importantly, recent in situ lineage tracing studies conducted in both mice and humans have provided further solid evidence for prostate stem/progenitors11,12,13,14,15,16. The basal cell compartment in many epithelial tissues including the epidermis17,18, colorectum19, bladder20,21 and mammary gland22,23 has been reported to enrich with stem cells. Using the K14rtTA/TetOCRE/RosaYFP or K5CREER/RosaYFP pan-basal cell lineage tracing mice, it has been shown that multipotent basal stem cells can contribute to the differentiation of prostate basal, luminal and neuroendocrine cell lineages especially during early postnatal development13. From a study of quantitative clonal mapping and three-dimensional reconstruction of adult human prostates, Moad et al. recently reported that multipotent basal stem cells are exclusively located in juxta-urethral niches and function in a directed migratory way to generate epithelial progenitors24. In contrast, using pan-basal or pan-luminal cell lineage tracing approaches, it is shown that basal and luminal cell lineages are independent self-sustained during prostate regeneration in adult mice12. On the other hand, despite the long-term postulation that basal cells contain more primitive prostate stem cells due to their resistance to castration, capability to differentiate into basal, luminal and neuroendocrine cell lineages of the prostate epithelium, and susceptibility to oncogenic transformation4,5,13,25,26,27,28,29, the identity and nature of the prostate basal stem cells (PBSCs) within the heterogenous basal cell epithelia have not been elucidated. In addition, the existing markers for basal stem cells (CD117, CD133, CD44, Trop2, CD49f, Sca1, etc.)4,5,6 are found in both the basal and luminal cell compartments.
In the present study, we identify a PBSC subpopulation that expresses Zeb1, an important EMT inducer30,31, through both in vitro and in vivo functional, lineage tracing and genetic ablation analyses. Furthermore, we integrated single-cell RNA sequencing with computational data analysis, a powerful approach that is lately developed to map cellular diversity of a given tissue, i.e., cortical neurons32 and cancer related immune cells33, etc., to examine the prostate basal cell compartment. Our single-cell transcriptomics data provide additional supporting evidence for the existence of Zeb1-expressing PBSCs and uncover their gene expression profile.
Results
Zeb1 is expressed in a prostate basal cell subpopulation
EMT has been previously considered to occur only in early embryonic development or pathological conditions, including tumor metastasis and wound healing34. Expression and function of core transcriptional factors such as Zeb1/2, Snai1/2, Twist1/2 which are required for EMT induction, have not been examined in normal prostate epithelia under physiological condition. Compared to prostate luminal cells, basal cells expressed low levels of epithelial-cell specific genes such as miRNA200 family35 and E-cadherin7 and exhibited a more mesenchymal-like phenotype. We therefore asked whether core EMT inducers are present in normal prostate basal cells. Immunofluorescent analysis of prostate sections showed that less than 1% of basal cells were positively labeled for Zeb1 immunostaining (Fig. 1a, b). Snai1 or Slug (Snai2) was expressed in 20% or 90% of p63+ basal cells, respectively, while Twist1/2 positive staining was not detected in the basal layer (Fig. 1a, b). Considering the interesting expression pattern of Zeb1 in the prostate epithelium and the previously reported role of Zeb1 in stemness acquisition and maintenance of cancer stem cells (CSC) characteristics31,36,37, we decided to investigate the biological relevance of Zeb1+ basal cells in the prostate.
To better characterize and to more easily enrich the Zeb1+ prostate epithelial cells, we established a tdTomato knockin mouse model in which the coding sequence of tdTomato fluorescent reporter was linked to the last exon of Zeb1 by a P2A element (Fig. 1c). Using immunofluorescent triple-staining of RFP, Zeb1 and CK14 on mouse prostate sections, we confirmed that the tdTomato labeling faithfully reflected the endogenous expression of Zeb1 (Fig. 1d). TdTomato positive cells were only found in prostate basal cells (marked by CK5 immunostaining) but not from luminal cell compartment (labeled by CK8 immunostaining) (Fig. 1e). The Zeb1+/tdTomato+ prostatic basal cells were more frequently detected in the urethra-proximal region relative to the distal region, the location where prostate stem cells were suggested to reside4,38,39 (Fig. 1f, h). Moreover, Zeb1+ basal cells located in the proximal region were more proliferative than those in the distal region (Supplementary Fig. 1a, b). In addition, we found that the percentage and absolute number of Zeb1+/tdTomato+ basal cells declined as the prostate development proceeded (Fig. 1i and Supplementary Fig. 1c). We then examined the dynamics of Zeb1+ basal cells during prostate regression and regeneration. We first checked the impact of castration and androgen replacement on bulk CK5+ basal cells and observed a moderate decrease of total basal cell number upon castration and a recovery of basal cell number after regeneration (Supplementary Fig. 2a). In contrast, as shown in Fig. 1g, j and Supplementary Fig. 2b–d, the proportion and absolute number of the Zeb1+ CK5+ population moderately augmented in regressed prostates, and then decreased to the intact prostate level after regeneration. We detected an increase of Ki67 positive cells in Zeb1+ basal cells from the proximal region of prostates in castrated mice, suggesting that the increase of Zeb1+ basal cell number may at least be partially contributed from cell proliferation (Supplementary Fig. 2e, f).
Zeb1+ basal cells are enriched for prostate basal stem cells
To test the role of Zeb1+ basal cells in prostate development, we performed a prostate organoid-forming assay in vitro using flow cytometry sorted Lineage− Sca-1+ CD49fhigh (LSC)Zeb1+ and LSCZeb1− cells (Fig. 2a, b). While few and small organoids were produced from LSCZeb1− cells, significantly larger and more organoids were generated from LSCZeb1+ cells (Fig. 2c, e). Immunostaining analysis of frozen sections of organoids formed from sorted LSCZeb1+ cells showed generation of both basal (CK5, CK14 or p63 positive) and luminal (CK8 or AR positive) cells (Fig. 2d). Moreover, LSCZeb1+ cells possessed a serial organoid forming capacity, indicating a self-renewing characteristic (Fig. 2e). According to the gross appearance and the H&E staining of organoid sections, LSC cell-derived organoids can be divided into three types, the acinar, compact or lumen phenotypes (Fig. 2f, g). On the other hand, we performed organoid sectioning and immunostaining to further analyze the organoid phenotype and observed that both basal-only (p63+ CK8−) and multipotent (p63+ CK8+) organoids can be generated from prostate LSC cells (Fig. 2h, i). We could detect both tdTomato+ (Zeb1+) and tdTomato− (Zeb1−) cells in the organoids derived from LSCZeb1+ cells. The percentage of tdTomato+ (Zeb1+) cells remained stable along serial passages (Source data file), suggesting that LSCZeb1+ cells can undergo self-renewal and differentiation. In addition, we performed organoid forming assays using LSCZeb1+ and LSCZeb1− cells under androgen deprived condition. The number of organoids formed from Zeb1+ cells (especially with the multipotent phenotype) was significantly more than organoids formed from Zeb1− cells following androgen deprivation (Supplementary Fig. 2g–i).
We then evaluated the potential of LSCZeb1+ cells to generate prostates in vivo, a gold standard to assess the stem cell phenotype4,40,41. Using an in vivo kidney capsule transplantation assay, we grafted 1000 LSCZeb1+ or LSCZeb1− mouse prostate cells in combination with rat embryonic urogenital sinus mesenchymal (UGM) stromal cells under the renal capsule of host athymic nude mice. Compared to opaque and small grafts derived from LSCZeb1− cells, LSCZeb1+ cells generated semi-translucent and large grafts (Fig. 3a–c). Histological analysis indicated that Zeb1+ grafts underwent ductal morphogenesis with differentiation of basal (CK5+), luminal (CK8+) and rare neuroendocrine (Syp+) cell lineages (Fig. 3d, e). Pbsn expression further confirmed differentiation of functional secretory luminal cells (Fig. 3e). In addition, Zeb1+ grafts expressed the mouse β-integrin, which validated their mouse origin and indicated that they were not produced from the contamination of rat epithelial cells from the preparation of rat UGM cells (Fig. 3e). We further verified that the generated prostate tissues were originated from Zeb1+ donor cell grafts by positive staining of an MHC class I haplotype H-2kb protein that is specifically expressed in C57BL/6 but not host athymic mouse cells (Fig. 3e). The unstructured tissue generated from LSCZeb1− cells was comprised of basal cells (CK5+), rare luminal cells (CK8+) and implanted rat urogenital sinus mesenchymal cells (mouse β-integrin− and Vimentin+) (Fig. 3f, g). These data suggested that LSCZeb1+ cells are multipotent prostate stem cells and possess the capacity of generating functional prostates.
Single Zeb1+ basal cell can generate functional prostate
We then wondered whether a single Zeb1+ basal cell would generate a prostate in vivo. Single viable LSCZeb1+ or LSCZeb1− cell was FACS sorted into individual well of a 96-well plate, mixed with rat UGM stromal cells and transplanted into the renal capsules of nude mice. Each well was examined under the microscope to confirm the presence of a tdTomato+ or tdTomato− single cell (Fig. 3h). Fifteen prostates with well-differentiated prostate epithelial tubules containing all three basal, luminal and neuroendocrine cell lineages were generated from 36 single LSCZeb1+ cell transplants (Fig. 3i–l). In contrast, only 3 prostates were produced from 33 single LSCZeb1− cell xenografts (Fig. 3i–l). Furthermore, Zeb1+ Epcam+ cells harvested from the first generation of xenografted prostate tissues were able to form functional prostates in the secondary and tertiary sub-renal capsule transplantations (Fig. 3m, n). The percentage of tdTomato+ (Zeb1+) cells remained stable along serial implantation, suggesting that the LSCZeb1+ cell can undergo self-renewal and differentiation (Fig. 3o, p). Collectively, these data provided additional direct evidences that LSCZeb1+ cells represented a multipotent prostate stem cell population.
Lineage tracing supports multipotency of Zeb1+ basal cells
To further determine the role of Zeb1+ basal cells in prostate development in vivo, we generated a Zeb1-CreERT2 mouse line and crossed it with Rosa-CAG-LSL-tdTomato mice to trace Zeb1+ basal cells during the postnatal prostate development (Fig. 4a, b). Tamoxifen administration to postnatal day 3 Zeb1-CreERT2/tdTomato mice induced expression of tdTomato in 10% of Zeb1+ cells (Fig. 4c). Consistent with our immunostaining results and findings from the Zeb1-tdTomato knockin mouse model (Fig. 1), about 0.33% of CK5+ basal cells were labeled by tdTomato but none of the CK8+ luminal cells nor Syp+ neuroendocrine cells were labeled at 2 days after induction (Fig. 4d–f, i). Interestingly, 12 days after the tamoxifen administration, we found clusters of CK5+ CK8− tdTomato+ cells located in the outer basal cell layer as well as CK5− CK8+ tdTomato+ cells in the inner luminal compartment (Fig. 4g, i). Quantitative analysis exhibited that labeled basal cells expanded more than 16 folds and tdTomato expressing luminal cells increased from zero to around 4% in 12 days. Meanwhile, we also observed that about 3% of Syp+ neuroendocrine cells were marked with tdTomato at 12 days post induction (Fig. 4h). Those results further substantiated the notion that Zeb1+ basal cells were able to generate basal, luminal and neuroendocrine cell lineages.
To explore the role of Zeb1+ prostate basal cells during tissue regression and regeneration, we performed lineage tracing experiments on Zeb1 expressing epithelial cells in adult mice. Tamoxifen was given to adult Zeb1-CreERT2/tdTomato mice to induce the expression of tdTomato in Zeb1+ cells (Supplementary Fig. 3a). As shown in Supplementary Fig. 3b–d, about 0.34% of CK5+ basal cells were labeled by tdTomato but none of the CK8+ luminal cells nor Syp+ neuroendocrine cells were labeled at 2 days after induction. Mice were then castrated at 1 week after the injection of tamoxifen and reconstituted with testosterone pellets at 3 weeks post castration. Unlike the multilineage differentiation of Zeb1+ basal cells in early development, we found that Zeb1 + basal cells gave rise to mostly basal cells but not luminal or neuroendocrine cells based on lineage tracing analysis in prostate regeneration (Supplementary Fig. 3e, f).
In addition, we performed qRT-PCR assays for previously reported surface markers of stem/progenitor cells in the prostate or mammary gland4,6,24,42,43,44. We found that CD117, CD133 and Procr were expressed with an enrichment of more than 2 folds in the LSCZeb1+ versus the LSCZeb1− cells (Supplementary Fig. 4a). The expression levels of CD166, Trop2 and Dlk1 were slightly higher in the LSCZeb1+ than the LSCZeb1− cells (Supplementary Fig. 4a). Additionally, we conducted flow cytometric staining of LSCZeb1+ cells and found that CD117, CD133, Procr, Trop2, and Dlk1 were presented in 23.18%, 49.83%, 69.38%, 21.43%, 12.43% of the LSCZeb1+ cells respectively (Supplementary Fig. 4b). A combination of the top expressing markers in LSCZeb1+ cells, that are CD133 and Procr, cannot very well isolate the LSCZeb1+ cell population (Supplementary Fig. 4c).
Zeb1 is required for development of prostatic basal cells
We then asked whether Zeb1 was functionally required for normal prostate development. To that end, we first utilized the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system to achieve Zeb1 gene knockout in prostate organoids in vitro (Fig. 5a). Efficient Zeb1 deletion in mouse primary prostate epithelial cells was validated by immunoblotting (Fig. 5b). Zeb1 sgRNA transfected prostate organoids contained CK8+ luminal cells, however, Zeb1 knockout organoids displayed a marked decrease of p63 expressing basal cells compared to organoids formed from mock-transfected prostate epithelial cells (Fig. 5c, d). We next investigated the role of Zeb1 in prostate development in vivo. Due to the fact that Zeb1 knockout mice died shortly after birth45, UGS from E16 Zeb1−/− embryos were dissected and transplanted beneath the renal capsule of male athymic mice to allow us to assess prostate epithelial development with Zeb1 deletion (Fig. 5e). Although Zeb1−/− UGS grafts developed into prostate and contained prostatic ductal structures, Zeb1 null grafts were smaller (Fig. 5e, f). Detailed histological examination revealed that approximately 50% of the Zeb1−/− UGS derived prostate epithelia virtually displayed all luminal cells that are positive of CK8 and AR without basal cells. In the other prostate epithelia, basal cell numbers were greatly reduced (Fig. 5f–h). Further immunofluorescent co-staining with Zeb1 and p63 antibodies affirmed Zeb1 deletion and marked decrease of basal cells in Zeb1−/− UGS grafts (Fig. 5g, h). These findings were further supported with additional staining of other basal cell markers CK5 and CK14 (Fig. 5g, h). Syp-expressing neuroendocrine cells could still be found in Zeb1−/− UGS derived prostate epithelium (Fig. 5g). Collectively, our in vitro and in vivo data highlight an indispensable role for Zeb1 in prostate basal cell development.
Single cell RNA-seq confirms the Zeb1+ basal cell subset
To provide additional evidence for the existence of a Zeb1+ basal population, we performed unbiased single cell transcriptomic analysis of prostate basal cell-enriched LSC cell population. Using the 10x Genomics single cell system followed by Illumina sequencing, prostate LSC cells were purified from mouse prostates via FACS for single-cell RNA sequencing. Sorted LSC cells were confirmed by q-RT-PCR and immunostaining for expression of basal cell markers (Supplementary Fig. 5a–c). The flow chart for single-cell RNA sequencing data analysis was included in Supplementary Fig. 5d. To visualize cellular heterogeneity, 9833 single-cell transcriptome data were subjected to unsupervised Seurat clustering and projected onto two dimensions by t-distributed stochastic neighbor embedding (t-SNE). After removal of low-quality and contaminated non-epithelial cells (Supplementary Figs. 5e, f, 6), this led to a total of 9 prostate LSC cell clusters (Cluster 1–9 or termed as C1–C9) (Fig. 6a). Importantly, we further found that while all other LSC cell clusters displayed high levels of epithelial genes, the cell cluster C7, expressed both epithelial markers Epcam, Cldn1, Ocln and stromal cell markers including Vim, Fn1, and Acta2, stood out uniquely among all the cell clusters (Fig. 6a, b). Detailed analysis of highly variable genes in C7 showed that Zeb1 was exclusively expressed in C7 (Fig. 6a, b). Other EMT transcriptional factors including Zeb2, Snai1, Prrx1 and Prrx2 were also highly transcribed in C7 (Fig. 6a–c). We performed unbiased GSEA using differential expressing genes in Cluster 7 versus all the other cell clusters. As shown in Supplementary Fig. 7a, b, GSEA analysis indicated that epithelial markers were downregulated and mesenchymal markers were positively enriched in the Cluster 7. Moreover, previously reported stem cell-related signatures were significantly upregulated in the cell cluster 7 (Supplementary Fig. 7c). Collectively, the unbiased examination of single-cell gene expression profiles of prostate LSC cells further substantiates a unique Zeb1 expressing LSC cell subpopulation with the mesenchymal and stem cell gene signature. Interestingly, when we used statistical and computational frameworks to delineate the differentiation states of the 9 cell clusters, both monocle and diffusion map scripts indicated that C7 was at one of the tips in the lineage tree (Supplementary Fig. 7d–g). We therefore chose cluster 7 to serve as the beginning point to delineate the developmental trajectory using the Slingshot script. Three lineage trajectories originated from C7 toward committed basal cells or “intermediate cells” that expressed both basal and luminal cell signatures were revealed (described in more detail in Supplementary Fig. 8). Collectively, the unbiased examination of single-cell gene expression profiles of prostate basal cells identified a unique Zeb1 expressing LSC cell subpopulation with the mesenchymal gene signature and indicated that it positioned at the beginning of differentiation trajectories.
Wnt signaling pathway is enriched in LSCZeb1+ cells
As an effort to uncover signaling pathways that are enriched in the cell cluster C7, we applied gene ontology (GO) enrichment analysis to the single-cell RNA-seq data. Coincidently, we found that the Wnt signaling pathway, which we have reported recently to promote self-renewal of prostate cancer stem cells46, was one of the top hits preferentially presented in the cluster C7 (Fig. 6d). GSEA analysis further substantiated that Wnt signaling pathway was positively enriched in the cluster7 (Supplementary Fig. 9a, b). The Wnt signaling pathway was demonstrated previously to play an essential positive role in inducing EMT47. Our RT-PCR experiments confirmed a significant upregulation of key Wnt signaling components in LSCZeb1+ cells (Supplementary Fig. 9c). Moreover, we tested the impact of Wnt signaling pathway agonist Wnt3a on the organoid-forming capacity of LSCZeb1+ cells and observed that Wnt3a (100 ng/ml)) significantly increased the size and number of prostate organoids (Supplementary Fig. 9d–f). These data further suggested a positive role of Wnt signaling in Zeb1+ organoids outgrowth. It was recently reported that Procr, a Wnt pathway target, marks a novel multipotent mammary stem cell population44. Consistently, we also found a moderate (1.5 folds) but significant upregulation of Procr in LSCZeb1+ prostate cells (Supplementary Fig. 9c). Further flow cytometry examination revealed that Zeb1+ cells represented only a small subpopulation (8%) of Procr expressing prostate epithelial cells (Supplementary Fig. 9g), suggesting a more enrichment of PBSCs in Zeb1+ than Procr+ prostate epithelial cells. Furthermore, examination of prostate sections from APCmin mice with the aberrantly activated Wnt pathway revealed a significant increase of Zeb1+ basal cells (Fig. 6e, f). We conducted additional immunostaining analyses and found that Zeb1+ basal cells entered a more actively proliferating state in APCmin mice, further suggesting that the Wnt signaling promotes the expansion of Zeb1+ basal cells (Fig. 6g, h).
Zeb1+ cells are found in mouse and human prostate epithelia
We next asked the influence of oncogenic transformation on the Zeb1 expression profile in the prostate epithelium. For that purpose, we crossed the Zeb1/tdTomato mice with the Hi-Myc prostate cancer mouse model. Immunofluorescent co-staining of Zeb1 or tdTomato together with basal or luminal cell markers revealed a 2-fold increase of Zeb1+ basal cells in Zeb1/tdTomato; HiMyc prostates (1.25% versus 0.73% in control mice at 3-month old, or 1.71% versus 0.71% in control mice at 5-month old) (Fig. 7a, b). Of note, we detected a small number of Zeb1+CK8+ luminal cells in HiMyc mice, which stood in contrast to the exclusive expression of Zeb1 in the basal cell compartment from wild type prostates (Fig. 7a, b). The pathology of Hi-Myc mice was evaluated by a pathologist and characterized in Supplementary Fig. 10. In addition, we examined the Zeb1+ epithelial cells in two other GEMM models of prostate cancer, the Probasin-Cre; Ptenfl/fl (PKO) model and the TRAMP model. Consistently, we detected an increase in Zeb1+ basal cells and emergence of Zeb1+ p63− or Zeb1+ CK5− cells in prostates of both tumor models (Supplementary Fig. 11). Similarly, positive immunostaining of Zeb1 protein could be found in a small percent of CK14+ basal cells in human prostate tissues (non-benign prostate hyperplasia (non-BPH) (n = 3) and BPH specimens (n = 7)) (Fig. 7c, d). During the examination of many sections of human prostate cancer samples, we infrequently found cancer tissues (Gleason score: 3 + 3 or 3 + 4, n = 4) with remaining CK14+ basal cells, in which Zeb1 positive staining could be found (Fig. 7c, d). Intriguingly, Zeb1+ CK8+ luminal cells were barely detected in non-BPH but could be observed occasionally in BPH specimens and more frequently in prostate cancer specimens (Fig. 7e, f). Together, Zeb1 expressing epithelial cells can be detected in both basal and luminal layers of prostates from Hi-Myc mice and in human prostate samples.
Discussion
The prostate basal cell compartment is suggested to contain stem/progenitor cells4,5,13,29, but the stem cell subpopulation within prostate basal cells and its transcriptional profile remain largely unknown. We here discover the existence of Zeb1-positive prostate epithelial cells in a small population of basal compartment but not in the luminal layer. Zeb1+ basal cells are more frequently found in the stem cell enriched urethra-proximal region. Importantly, utilizing functional methods and in vivo lineage tracing, we prove that the Zeb1+ prostate basal cells can self-renew and possess multipotency to generate all three prostatic epithelial cell lineages in vivo. Furthermore, in vitro and in vivo genetic ablation experiments showed a requirement of Zeb1 in basal cell differentiation. The unique Zeb1 expressing prostate basal cell cluster with mesenchymal cell features was further supported by single-cell RNA sequencing data. Our study underscores the importance of Zeb1 as a marker and an essential regulator for PBSCs.
EMT has been demonstrated to be utilized by tumor initiating cells to acquire stem cell attributes31. We find here that Zeb1 marks a population of PBSCs in prostates with mesenchymal features and higher expression levels of EMT inducers such as Snai1, Zeb2, Prrx1 and Prrx2. Support for our data comes from recent studies in mammary glands that multipotent Procr+ mammary stem cells display EMT characteristics and that EMT is required for the tissue-reconstitution activity of mammary basal cells44,48,49,50. Together, those observations demonstrate that like what was shown for tumor initiating cells, EMT is also closely associated with stemness in normal prostate and mammary stem cells. However, there is also tissue specific differences between stem cells in prostate versus mammary tissues. Firstly, we find that although most Zeb1+ prostate basal cells express Procr, Procr is present in a much larger population of prostate epithelial cells containing both basal and luminal cells (Supplementary Fig. 9g), suggesting that Zeb1 appears to be a better maker for basal stem cells in prostates than Procr. Secondly, Zeb1 and Snai1 are expressed in prostate basal cells but not in mammary epithelial cells48,49,50, indicating distinct EMT inducing transcriptional factors or EMT programs are involved in respective stem cells.
Lineage tracing experiments conducted in early postnatal development in the current study demonstrate that Zeb1+ basal cells can self-renew and give rise to basal, luminal and neuroendocrine cell lineages. However, unlike the multilineage differentiation of Zeb1+ basal cells in early development, we found that Zeb1+ basal cells gave rise to mostly basal cells but not luminal or neuroendocrine cells in adult prostates regeneration. Although the absence of tdTomato tracing in regenerated luminal cells may be caused by a low Cre-ERT2 induction efficiency, these data suggested a stage dependent function of Zeb1+ basal cells, and were largely consistent with previous findings reported by professor Cédric Blanpain’s group using pan-basal cell K5CRE-ER/RosaYFP and K14rtTA/TetO-CRE /RosaYFP lineage tracing mice. Loss of function experiments performed on prostate organoids and UGS transplants show that Zeb1 knockout results in a remarkably decrease in the basal cell compartment, but luminal and neuroendocrine cell differentiation are largely unaffected. Consistently, previous studies on renal capsule transplants from p63-/- UGS exhibit development of luminal and neuroendocrine cells independent of basal cells51. In addition, lineage tracing experiments on CK14, CK8, CK5 and NKX3.1 cells support the existence of self-sustained luminal cell progenitors11,12,13,15. Therefore, both multipotent Zeb1+ basal stem cells and stem/progenitor cells from the luminal compartment contribute to prostate development. In addition, compared to the sharp contrast between Zeb1+ and Zeb1− basal cells, the difference between Zeb1 wild-type and Zeb1 knockout cells in the renal capsule transplantation assay is less drastic, although the size of prostate tissue produced from Zeb1 knockout cells is significantly smaller and the basal cell differentiation is severely compromised. Our interpretation is that the self-renewal and differentiation of Zeb1+ prostate basal stem cells are regulated by complicate and intricate molecular mechanisms. Zeb1 itself is an important player in it but not the only one. Other signaling molecules, transcriptional factors, or epigenetic modifiers may also be required.
At different developmental stages, castration-mediated regression and regeneration stages, we consistently observed that Zeb1+ basal cells are more enriched in the urethral-proximal region. In addition, we found that there was a gradient of cell proliferation activity in Zeb1+ basal cells from proximal to intermediate to distal region. This is somehow similar to a “directional flow” phenotype of human prostate basal stem cells as reported recently24. However, without real-time 3D imaging or 3D lineage tracing, it is not conclusive at this moment whether Zeb1+ basal stem cells function in a “direction flow” way in mouse prostates as reported in human prostates. In addition, we want to point out that Zeb1− basal cells can also form organoids, although with a much lower efficiency. Therefore, Zeb1+ basal cells were enriched for basal stem cells, but Zeb1− basal cells also have a degree of progenitor activity.
Intriguingly, we find that different from the exclusive basal expression of Zeb1 in wild-type prostate, Zeb1+ epithelial cells can be detected in the luminal compartment in Hi-Myc mice or in human prostate hyperplastic or tumor samples. There are two possible explanations for the appearance of Zeb1+ luminal cells: It could be derived from transformed Zeb1+ basal cells; or alternatively, the transformed luminal cells may acquire Zeb1 expression. Clarification of these two possibilities needs further investigations. Nevertheless, the appearance of Zeb1+ luminal cells at early stages of prostate tumorigenesis is of special interest, because most clinical prostate cancers display a dominant luminal phenotype by loss of basal cell phenotypes, and Zeb1 has been well shown in the acquisition of cancer stem cell properties36,52. This Zeb1+ luminal cell population might represent prostate tumor initiating cells, which can be also related to two different cellular origins for prostate cancers: basal versus luminal cells.
Single cell RNA analysis or RNA-seq has emerged as a very powerful tool to analyze heterogenous cell population of a tissue or organ. Previous studies have reported the composition of prostate epithelia in adult prostate from mouse and human prostate via single-cell q-PCR or RNA-seq. Utilizing a Fluidigm multiplex qPCR-based single-cell expression analysis platform, Joao D. Barros-Silva and et al examined the cell component of prostates from hormone naïve and castrated mice. They found that LY6D+ luminal cells are castration resistant prostate luminal progenitors53. Through single-cell RNA sequencing and flow cytometry, Gervaise H. Henry and et al specified markers for human prostate epithelial and stromal cell compartments54. To our knowledge, this work is the first report to decipher the heterogeneity within the prostate basal epithelium using unbiased single-cell transcriptomics. Importantly, along with the single-cell RNA-seq data, our immunocytochemical staining, functional experiments, and cell lineage tracing analysis together support that Zeb1 marks a multipotent prostate basal stem cells and Zeb1 itself is required for prostate basal cell development. Via analyzing different developmental stages, we have provided insights into the evolution of a unique Zeb1 expressing prostate stem cell subpopulation from embryonic development to postnatal development and to adulthood. The identification of the specific Zeb1+ prostate stem cell, its transcriptome profile and its expression pattern during prostate tumorigenesis could have important implications for our understanding of tissue development and regeneration and for identification of potential cellular origin for cancer.
Methods
Animals
Pregnant SD rats (E17) and athymic nu/nu male mice (7 weeks old) were purchased from Shanghai Slac laboratory animal company. Zeb1/tdTomato reporter mice were generated by the CRISPR-Cas9 method at Model Animal Research Center of Nanjing University. The sgRNA sequence we used is GAGGTTGGAGCTGCACAGCAGG. Exon 8 of Zeb1 followed by a P2A and tdTomato coding sequence, which was flanked by approximately 1.5 kb upstream and 1.5 kb downstream sequence of Zeb1 Exon 8, was cloned to a donor vector. Plasmid containing sgRNA and Cas9 expressing elements and the donor vector were injected to fertilized C57/B6 mouse eggs. F0 generation of mice were genotyped by sequencing and PCR. Positive F0 mice were used to backcross with C57/B6 mice to produce F1 generation of knockin mice. PCR Primers for Zeb1/tdTomato mouse genotyping were provided in Supplementary Table 1. Zeb1-CreERT2 mice were generated by a homologous recombination method at the Cyagen Biosciences Inc. The TAG stop codon of the last exon of Zeb1 was replaced with the “2A-CreERT2” cassette. Homology arms of the targeting vector were generated by PCR using BAC clone RP23-51G9 or RP23-207F18 from the C57BL/6 J library as template. The targeting vector contained the Neo cassette flanked by SDA (self-deletion anchor) sites and DTA for negative selection. C57BL/6 ES cells was used for gene targeting. F0 generation of mice were validated by sequencing and PCR. PCR Primers for genotyping of Zeb1-CreERT2 mice were provided in Supplementary Table 1. Zeb1 knockout with deletion of exon 1 mice was purchased from RIKEN BioResource Research Center. The Rosa-CAG-LSL-tdTomato mice were purchase from the Jackson laboratory. Hi-Myc mice were introduced from the National Cancer Institute (NCI:01XF5). The pathology of Hi-Myc mice was evaluated by a pathologist at Ren Ji hospital, affiliated with School of Medicine, Shanghai Jiao Tong University. Apcmin mice were introduced from the Nanjing Biomedical Research Institute of Nanjing University (T001457). All animal experiments were conducted according to the ethical regulations of Ren Ji Hospital. Animal experiment protocol were approved by the Ren Ji Hospital Laboratory Animal Use and Care Committee.
Prostate single cell preparation
Mouse prostates were harvested, minced, then digested with pre-warmed 1X collagenase/hyaluronidase solution (STEMCELL Technologies, #07912) for 3 hr at 37 °C, washed with PBS buffer once and placed in 0.25% Trypsin/EDTA for 6 min at 37 °C. FBS supplemented with 4% FBS was added to quench trypsin reaction followed by centrifuging at 350 g for 5 min. Then, the cell pellet was resuspended with pre-warmed Dispase/DNase I solution (STEMCELL Technologies, #07913, #07900) to thoroughly dissociate cells. Single cell suspension was obtained from passing through a 40 µm cell strainer.
Flow cytometry
Mouse prostate single cell suspension was blocked with the Fc blocker (CD16/32 antibody from eBioscience) for 40 min at room temperature. Staining antibodies were diluted in 4% FBS buffer containing Y-27632 ROCK inhibitor (STEMCELL Technologies, #72302) and applied to the prostate single cell suspension for 40 min at room temperature. Flow cytometry analysis or sorting was conducted using BD Accuri C6 or FACSAria III flow cytometer. Antibodies used in the study are listed in Supplementary Table 2. Propidium Iodide (PI, Invitrogen) was added to sample before sorting to gate viable cells.
Single cell RNA-seq and data analysis
Lineage− Sca-1+ CD49fhi PI− prostate cells from postnatal day 14 mice were sorted and counted manually before processing to the single cell RNA-seq library preparation. Libraries were constructed following the instruction of the Chromium single cell 3’ solution (10x Genomics). The libraries were sequenced on Illumina Hiseq X Ten platform. 10x Genomics workflow and Cell Ranger Single Cell Software was used to process raw sequencing data and to align reads to the mm10 mouse reference genome. Raw gene expression matrix produced by 10x Genomics workflow were first processed by the Seurat package. Cells with less than 5000 unique molecular identifiers (UMIs) or less than 1400 genes detected or more than 5% UMI mapped to mitochondria genes were removed (Supplementary Fig. 5e, f). This led to 9833 high-quality single-cell RNA-seq data with an average gene detected to be around 2703 (Supplementary Fig. 5f). 1606 variable genes were selected based on their expression and dispersion (expression cutoff = 0.0125 and dispersion cutoff = 0.5). The first 12 principal components were used for clustering analysis (resolution = 0.5) and t-SNE projection. This identified a total of 10 clusters (Supplementary Fig. 6a). Violin plots of epithelial, stromal, endothelial and immune cell associated genes for those 10 different cell clusters were presented in Supplementary Fig. 6b–m. Clusters which expressed clear markers of non-epithelial cells were removed in the following analyses. Cluster 5 was labeled as endothelial cells based on the expression of Eng, S1pr1 and Emcn (Supplementary Fig. 6i–k). Cluster 10 was labeled as immune cells based on the expression of Cd74 and Cd72 (Supplementary Fig. 6l, m). After the removal of above-mentioned non-epithelial cells, we re-ran the Seurat work flow to generate new cell clusters and t-SNE projections (the first 12 principal components were used). A total of 9 clusters were identified (Fig. 6a). The expression levels of prostate basal cell markers, Krt5 and Krt14 were shown in violin plots (Supplementary Fig. 6n, o). We used the FindAllMarkers script in the Seurat package to identify genes that are enriched in a specific cluster (the specific cluster vs. the rest of clusters) with default settings. PCA matrix with the first 12 principal components and clusters from the second Seurat running were taken as input. The cluster representing putative stem cells (Zeb1 expressing cluster 7) was chosen as the root node. Totally, three branches were identified. GSEA analysis was performed by using fgsea package in R55 with ranked genes fold change values created by FindMarkers module (default parameter except min.pct = 0.2) in Seurat package56. Gene Sets used here were collected from previous publications57,58,59,60,61. The curated gene sets (C2) of the Molecular Signature Database (MSigDB) version 6.2 provided by the Broad Institute (http://www.broad.mit.edu/gsea/)62. Similar clusters and the structure of a common origin could also be inferred by using Monocle or Diffusionmap with standard parameters (Supplementary Fig. 7). Codes used are deposited to the website [https://github.com/HelenHeZhu/StemCell].
Human prostate clinical specimens
Freshly dissected human prostate specimens (non-BPH, BPH and prostate cancer specimens) were obtained from the department of Urology at Ren Ji Hospital with informed consent from patients. All Human sample experiments were conducted according to the ethical regulations of Ren Ji Hospital. Human sample collection and handling protocols were approved by the Ren Ji Ethics committee.
UGM stromal cell preparation
The UGM isolation procedures has been described previously63. Briefly, E17 embryos from pregnant SD rats were sacrificed and urogenital sinuses were collected. The UGM was separated from the urogenital sinus epithelium and then digested with 1X collagenase/hyaluronidase solution on a shaker for 90 min at 37 °C. The UGM was washed with PBS once and then placed in 0.25%Trypsin/EDTA for 6 min at 37 °C. UGM single cell suspension was seeded to cell culture dishes and cultured in DMEM (Thermo Fisher Scientific, C11965500BT) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100U/ml penicillin and 100 mg/ml streptomycin in vitro for at least 1 week.
Serial isolation and renal capsule transplantation
We used a previously described procedure to isolate the primary prostate epithelial cells and to perform renal capsule transplantation63. For the multi-cellular renal capsule assay, 1,000 Lineage− Sca-1+ CD49fhi tdTomato+ or Lineage− Sca-1+ CD49fhi tdTomato− prostate basal cells were sorted via FACS and then mixed with purified rat UGM cells (250,000 cells per graft) in rat tail Collage Type I (4.42 mg/ml, Corning, 354236). Next, the mixture was plated as a drop of 10 µl into the center of a well of a 6-well plate. The plate was placed in a 37 °C cell culture incubator for at least 60 min to make sure that the Collage type I solidified. Then pre-warmed DMEM (containing 10% FBS) medium was gently pipetted into each well. Each collagen graft was embedded underneath the renal capsule of a nude mouse the next day. For the first renal capsule transplantation, single Lineage− Sca-1+ CD49fhi tdTomato+ or Lineage− Sca-1+ CD49fhi tdTomato− prostate basal cell was sorted into each well of a 96-well plate via FACS and then examined under a light microscope. The viable sorted cell was mixed with purified rat UGM cells (250,000 cells per graft) in rat tail Collage Type I (4.42 mg/ml, Corning, 354236). For the secondary or tertiary renal capsule transplantation, grafts from last transplantation were digested with 1X collagenase/hyaluronidase solution for 90 min and 0.25% Trypsin/EDTA for 6 min at 37 °C. 50 Lineage− Epcam+ Zeb1+ or Lineage− Epcam+ Zeb1− cells were sorted via FACS and then mixed with UGM stromal cells (250,000 cells per graft). All serial transplantation grafts were harvested at 8 weeks after implantation. UGS from Zeb1−/− and Zeb1+/+ embryos were implanted under the renal capsule of male nude mice. The transplants were collected at 20 days post implantation and sectioned for further analysis.
Cell lineage tracing
To trace the fate of Zeb1+ basal cells, Zeb1-CreERT2/tdTomato mice were administrated with 1.25 mg tamoxifen (40 mg/mL solution, dissolved into corn oil, Sigma) via intraperitoneal injection at postnatal day 3. Two days and 12 days after the induction, prostates were collected and sectioned for immunofluorescence staining of different prostate epithelial cell markers. To examine the functional role of Zeb1+ basal cells during prostate regeneration in adults, adult Zeb1CreERT2/tdTomato mice were given tamoxinfen to induce expression of tdTomato in Zeb1+ cells. Four days after the induction, some of the mice were sacrificed and the prostates were collected and sectioned to examine the induction rate of the Cre recombinase. The rest of mice were castrated at 1 week after the injection of tamoxifen and reconstituted with testosterone pellets at 3 weeks post castration. Two weeks after the regeneration, prostates were collected and sectioned for immunofluorescent staining of different prostate epithelial cell markers to determine the differentiating fate of Zeb1+ basal cells.
Organoid culture
We utilized a previously reported protocol to culture organoids in vitro and perform organoid frozen sections for immunostaining analysis15. FACS purified LSCZeb1+ and LSCZeb1− cells were centrifuged at 350 g for 5 min at 4 °C and resuspended in complete organoid culture media (10 ng/ml epidermal growth factor, 10 μM Y-27632, 1x glutamax, 5% Matrigel, 5% charcoal-stripped FBS, 100 nM DHT, heat-inactivated at 55 °C for 1 hr before use) with the density of 5000 to 10,000 prostate epithelial cells per 100 μL media. The medium was refreshed every 3 days. After approximately 2 weeks, organoids were washed with cold PBS buffer repeatedly to remove the matrigel, collected via centrifugation at 350 g for 5 min, then dissociated using 0.25% Trypsin/EDTA for 7 min at 37 °C. The single cells were centrifuged and resuspended in medium following the aforehand described procedure for a serial passaging. For section preparation, organoids were collected, fixed with 4% PFA for 30 min, washed with cold PBS buffer twice, then centrifugated and resuspended with 50 μL collagen type I and incubated at 37 °C for 1 hour to solidify the organoid/collagen slurry. The slurry was transferred into 4%PFA solution for fixation, 30% sucrose for dehydration and finally O.C.T for embedding and section. Five-micrometer frozen sections were used for following immunostaining.
CRISPR/Cas9 mediated Zeb1 knockout prostate organoids
The lentiCRISPRv2-mCherry plasmid containing cas9 and Cherry fragments was purchased from Addgene (#99154, kindly deposited by Dr.Agata Smogorzewska). Then the Cherry fragment was exchanged for GFP fragment via enzymatic digestion and connection followed by sgRNA cloning. The single guide RNA sequences are ACTGCTTATATGTGAGCTAT in the upstream and GGAACAACCTGAAGTTGACT in the downstream of the sixth exon of the mouse Zeb1 gene. The Zeb1-cas9 lentivirus was transfected into primary prostate cells using a spinoculation method at 3,000 rpm and room temperature for 2 hours. Cells were then transferred to a 15 mL tube and spinned down at 400 g for 5 min. The pellet was resuspended with the complete organoid culture media and seeded at a density of 10,000 cells per well of a 96-well plate. After 3 weeks of culture, the organoids were harvested and dissociated with pre-warmed 0.25% Trypsin-EDTA. Single cell suspension was stained with anti-lineage (CD45.2, CD31, Ter119), anti-Sca-1 and anti-CD49f antibody. Lin− Sca1+ CD49fhi GFP+ cells (sgRNA successfully transfected prostate epithelial cells) were FACS sorted for organoid culture. Organoids were collected when reaching 200μm in diameter, and then fixed, dehydrated and embedded with O.C.T for frozen section preparation.
RNA extraction and quantitative-PCR analysis
Total RNA was extracted from FACS purified LSCZeb1+ or LSCZeb1− cells using a RNeasy micro kit (Qiagen). cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara, RR037A). qPCR was conducted using SYBR Premix Ex Taq (Takara, RR420A). Relative transcript abundance was quantified by the comparative CT method using Actin as an internal reference gene. Primers utilized in RT-PCR were shown in Supplementary Table 1.
Immunofluorescence staining
Human or mouse prostates were fixed in 4% paraformaldehyde for 20 min and dehydrated overnight in 30% sucrose solution. Tissues were embedded in Optional Cutting Temperature (O.C.T.) compound and placed in a −80 °C refrigerator for 10 min or longer depending on the tissue size. Frozen sections were cut at a thickness of 6 um. Sections were washed with PBS buffer and placed into 0.01 M sodium citrate (PH 6.0) buffer for heat-induced antigen retrieval. Slides were then subjected to a blocking step in 10% donkey serum solution for 1 h at room temperature. Primary antibodies, diluted in 1% donkey serum, were added to sections overnight at 4 °C. After thorough wash with PBS buffer, secondary antibodies were applied and incubated for 1 hr at room temperature. Sections were rinsed at least three times with PBS before being mounted by Vector Shield mounting medium containing DAPI. Antibodies (including the dilutions) used in the study is listed in Supplementary Table 2.
Immunohistochemistry
Paraffin-embedded prostate tissue sections were deparaffinized and rehydrated following conventional methods. Harris modified hematoxylin solution (Sigma) was applied to sections for 5 min followed by water washing. Then 1% Eosin solution (Sigma) was applied to sections for 3 min. After thorough wash, sections were mounted using neutral balsam.
Castration and androgen replacement
Zeb1 reporter mice were surgically castrated. Three weeks later, mice were given dihydrotestosterone (MCE, HY-A0120) dissolved in sterile corn oil via intraperitoneal injection twice a day (50 ug/d) for prostate regeneration. Prostate collected at indicated regeneration stages (regressed, regenerated, and recovered stages post testosterone administration) for analysis.
Statistical analysis
We used the ImageJ 1.46r software to quantify positive stained cells. All statistical analysis was evaluated using a two-tailed Student’s T-test and a p-value < 0.05 was defined to be significant.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The single cell RNA-seq raw data used for this study are available at the GEO web with the accession number GSE111429. Codes used are deposited to the website [https://github.com/HelenHeZhu/StemCell]. All other data is available in the main text or the supplementary materials. The source data underlying Figs. 1b, h–j, 2c, e–i, 3p, 4i, 5b, d, h, 6, 7 and Supplementary Figs. 1c, 2, 3f, 8, 9d–f, 11 are provided as a Source Data file.
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Acknowledgements
The study is supported by funds to W-Q Gao from the National Key R&D Program of China (2017YFA0102900), the National Natural Science Foundation of China (NSFC, 81630073 and 81872406), the Science and Technology Commission of Shanghai Municipality (16JC1405700), the Shanghai Jiao Tong University Scientific and Technological Innovation Funds and the KC Wong foundation, and by funds to H.H. Zhu from the NSFC (81772743 and 81972404), Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support (20181706), Shanghai Rising-Star Program (17QA1402100), the Shanghai Youth Talent Support Program, School of Medicine, Shanghai Jiao Tong University (Excellent Youth Scholar Initiation Grant 16XJ11003) and the Innovative Research Team of High-level Local Universities in Shanghai.
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H.H.Z. and W.Q.G. conceived the study; X.W. performed the experiments. H.X. and D.W. conducted bioinformatic analyses; Z.J. and J.W. assisted in data interpretation; C.C. and Y.S. helped in cell culture experiments; H.Z. helped microscopic imaging; X.L. performed mouse genotyping and breeding; Y.S. helped organoid culture; Y.H. assisted in animal experiments. L.F., B.D. and W.X. collected human prostate samples and assisted in histological analysis of human sample; H.H.Z., W.Q.G. and X.W. interpreted the data and wrote the manuscript.
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Wang, X., Xu, H., Cheng, C. et al. Identification of a Zeb1 expressing basal stem cell subpopulation in the prostate. Nat Commun 11, 706 (2020). https://doi.org/10.1038/s41467-020-14296-y
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DOI: https://doi.org/10.1038/s41467-020-14296-y
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