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Changing medium and passaging cell lines

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Abstract

Cell lines are widely used in biomedical research. This protocol describes the methods used routinely to change the medium and passage the cells. Medium changes keep the cells healthy by providing fresh nutrients, while cell passage or splitting is required to maintain cells in exponential growth. Despite the simplicity of the methods used, each cell line has idiosyncracies. Whether working with one or several cell lines, there is no substitute for knowledge of their needs, including the range of phenotypes and growth patterns under different physical and nutrient conditions. Given the necessary care and attention, most cell lines are easy to maintain and grow.

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References

  1. Schaeffer, W.I. Usage of vertebrate, invertebrate and plant cell, tissue and organ culture terminology. In Vitro 20, 19–24 (1984).

    Article  CAS  PubMed  Google Scholar 

  2. Freshney, R.I. (ed.) Culture of Animal Cells 4th edn. (Wiley Liss, New York, 2000).

  3. Southam, C.M. Homotransplantation of human cell lines. Bull NY Acad. Med. 34, 416–423 (1958).

    CAS  Google Scholar 

  4. Gugel, E.A. & Sanders, M.E. Needle-stick transmission of human colonic adenocarcinoma. N. Engl. J. Med. 315, 1487 (1986).

    Article  CAS  PubMed  Google Scholar 

  5. Lloyd, G. & Jones, N. Infection of laboratory workers with hantavirus acquired from immunocytomas propagated in laboratory rats. J. Infect. 12, 117–125 (1984).

    Article  Google Scholar 

  6. Tedder, R.S. et al. Hepatitis B transmission from a contaminated cryopreservation tank. Lancet 346, 137–140 (1995).

    Article  CAS  PubMed  Google Scholar 

  7. Hummeler, K., Davidson, W.L., Henle, W., LaBoccetta, A.C. & Ruch, H.G. Encephalomyelitis due to infection with Herpesvirus simiae (Herpes B virus); a report of two fatal, laboratory-acquired cases. N. Engl. J. Med. 261, 64–68 (1959).

    Article  CAS  PubMed  Google Scholar 

  8. UKCCCR. UKCCCR guidelines for the use of cell lines in cancer research. Br. J. Cancer 82, 1495–1509 (2000).

  9. Coecke, S. et al. Guidance on good cell culture practice. Altern Lab Anim 33, 261–287 (2005).

    CAS  PubMed  Google Scholar 

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Acknowledgements

We thank Ian Freshney for reading and commenting on this set of protocols.

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Authors and Affiliations

  1. Department of Surgery, Prostate Cancer Research Centre, Royal Free and University College London Medical School, 67 Riding House Street, London, W1W 7EJ, UK

    John R Masters

  2. National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, EN6 3QG, Hertfordshire, UK

    Glyn N Stacey

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  1. John R Masters
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  2. Glyn N Stacey
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Correspondence to John R Masters.

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The authors declare no competing financial interests.

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Masters, J., Stacey, G. Changing medium and passaging cell lines. Nat Protoc 2, 2276–2284 (2007). https://doi.org/10.1038/nprot.2007.319

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