Ten years of development in light-sheet microscopy have led to spectacular demonstrations of its capabilities. The technology is ready to assist biologists in tackling scientific problems, but are biologists ready for it? Here we discuss the interdisciplinary challenges light-sheet microscopy presents for biologists and highlight available resources.
References
Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E.H.K. Science 305, 1007–1009 (2004).
Huisken, J. & Stainier, D.Y.R. Opt. Lett. 32, 2608–2610 (2007).
Dodt, H.-U. et al. Nat. Methods 4, 331–336 (2007).
Wu, Y. et al. Nat. Biotechnol. 31, 1032–1038 (2013).
Chen, B.-C. et al. Science 346, 1257998 (2014).
Tomer, R., Khairy, K., Amat, F. & Keller, P.J. Nat. Methods 9, 755–763 (2012).
Krzic, U., Gunther, S., Saunders, T.E., Streichan, S.J. & Hufnagel, L. Nat. Methods 9, 730–733 (2012).
Schmid, B. et al. Nat. Commun. 4, 2207 (2013).
Pitrone, P.G. et al. Nat. Methods 10, 598–599 (2013).
Gualda, E.J. et al. Nat. Methods 10, 599–600 (2013).
Kaufmann, A., Mickoleit, M., Weber, M. & Huisken, J. Development 139, 3242–3247 (2012).
Desmaison, A., Lorenzo, C., Rouquette, J., Ducommun, B. & Lobjois, V. J. Microsc. 251, 128–132 (2013).
Wu, J., Li, J. & Chan, R.K.Y. Opt. Express 21, 14474–14480 (2013).
Capoulade, J., Reynaud, E.G. & Wachsmuth, M. in Imaging Marine Life (ed. Reynaud, E.G.) 186–209 (Wiley-VCH, 2013).
Preibisch, S., Saalfeld, S., Schindelin, J. & Tomancak, P. Nat. Methods 7, 418–419 (2010).
Preibisch, S. et al. Nat. Methods 7, 418–419 (2010).
Saalfeld, S., Cardona, A., Hartenstein, V. & Tomancak, P. Bioinformatics 25, 1984–1986 (2009).
Amat, F. et al. Nat. Methods 11, 951–958 (2014).
Eliceiri, K.W. et al. Nat. Methods 9, 697–710 (2012).
Acknowledgements
We thank all members of the ever growing light-sheet community who push this field forward. All participants, speakers and instructors of the first EMBO practical course on light-sheet microscopy in Dresden inspired us to get this piece written. We thank J. Oegema for Figure 3b and L. Gutierrez-Heredia for Figure 2b.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Reynaud, E., Peychl, J., Huisken, J. et al. Guide to light-sheet microscopy for adventurous biologists. Nat Methods 12, 30–34 (2015). https://doi.org/10.1038/nmeth.3222
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.3222
- Springer Nature America, Inc.
This article is cited by
-
Smart lattice light-sheet microscopy for imaging rare and complex cellular events
Nature Methods (2024)
-
Current and future applications of light-sheet imaging for identifying molecular and developmental processes in autism spectrum disorders
Molecular Psychiatry (2024)
-
Imagining the future of optical microscopy: everything, everywhere, all at once
Communications Biology (2023)
-
Superresolution structured illumination microscopy reconstruction algorithms: a review
Light: Science & Applications (2023)
-
Viewing life without labels under optical microscopes
Communications Biology (2023)