Abstract
We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.
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Acknowledgements
We thank members of the Caenorhabditis Genetics Center for providing the N2 strain used in our experiments, and B. Stern, A. Murray, A. Saltzman, Joe Calarco and members of the Calarco laboratory for comments on the manuscript. This work was supported by US National Institutes of Health Early Independence Award (1DP5OD009153) and additional support from Harvard University to J.A.C., by National Institutes of Health grant R01GM072551 to M.P.C., and a National Human Genome Research Institute Center of Excellence in Genome Sciences award to G.M.C. A.E.F. is supported by a Ralph Ellison/American Federation for Aging Research postdoctoral fellowship.
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A.E.F., K.M.E. and J.A.C. conceived of and designed experiments, with help from Y.B.T.; A.E.F. and J.A.C. assembled vectors; A.E.F. and J.A.C. performed microinjections and screened mutants; A.E.F., J.A.C. and Y.B.T. performed off-target genotyping analysis; A.E.F., K.M.E. and J.A.C. wrote the manuscript with input from Y.B.T., M.P.C. and G.M.C.
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Supplementary information
Supplementary Figures and Tables
Supplementary Figures 1–4, and Supplementary Tables 1 and 2 (PDF 5711 kb)
Supplementary Video 1
Movie of a wild-type (N2 strain) worm. (MOV 1114 kb)
Supplementary Video 2
Movie of an unc-119 worm created by Cas9-mediated gene disruption. (MOV 1739 kb)
Supplementary Video 3
Movie of a dpy-13 worm created by Cas9-mediated gene disruption. (MOV 1641 kb)
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Friedland, A., Tzur, Y., Esvelt, K. et al. Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods 10, 741–743 (2013). https://doi.org/10.1038/nmeth.2532
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DOI: https://doi.org/10.1038/nmeth.2532
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