Abstract
We present a methodology for the design, construction and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of post-construction modification afforded by this approach and the ever-increasing repository of components within the framework will help accelerate the development of functional genetic circuits for synthetic biology.
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Acknowledgements
This work was supported by the Howard Hughes Medical Institute and the Office of Naval Research Multidisciplinary University Research Initiative.
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K.D.L., R.B.A., A.S.K. and J.J.C. designed the study. K.D.L., R.B.A., R.J.K. and A.S.K. performed experiments. K.D.L., R.B.A., R.J.K., A.S.K. and J.J.C. wrote the manuscript. A.S.K. and J.J.C. supervised the project.
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Litcofsky, K., Afeyan, R., Krom, R. et al. Iterative plug-and-play methodology for constructing and modifying synthetic gene networks. Nat Methods 9, 1077–1080 (2012). https://doi.org/10.1038/nmeth.2205
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DOI: https://doi.org/10.1038/nmeth.2205
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