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Translating metabolic exchange with imaging mass spectrometry

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Abstract

Metabolic exchange between an organism and the environment, including interactions with neighboring organisms, is important for processes of organismal development. Here we develop and use thin-layer agar natural product MALDI-TOF imaging mass spectrometry of intact bacterial colonies grown on top of the MALDI target plate to study an interaction between two species of bacteria and provide direct evidence that Bacillus subtilis silences the defensive arsenal of Streptomyces coelicolor.

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Figure 1: Thin layer agar natural product MALDI imaging.
Figure 2: Time course IMS of Bacillus subtilis strains and Streptomyces coelicolor cohabitation.

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Acknowledgements

The authors acknowledge M. Burkart (University of California, San Diego) for S. coelicolor A3(2). The funding was provided by the Beckman Foundation, the annotation of the cyclic peptides in this study was supported by NIHGM086283 (P.C.D.) and P.S. acknowledges financial support from Texas A&M University.

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Author notes

  1. Yu-Liang Yang and Yuquan Xu: These authors contributed equally to this work.

Authors and Affiliations

  1. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, USA

    Yu-Liang Yang, Yuquan Xu & Pieter C Dorrestein

  2. Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA

    Paul Straight

  3. Department of Pharmacology and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA,

    Pieter C Dorrestein

  4. Center for Marine Biotechnology and Biomedicine, University of California, San Diego, La Jolla, California, USA

    Pieter C Dorrestein

Authors
  1. Yu-Liang Yang
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  2. Yuquan Xu
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  3. Paul Straight
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  4. Pieter C Dorrestein
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Contributions

Y.-L.Y., Y.X. and P.C.D. prepared bacterial cultures and performed IMS. Y.-L.Y., Y.X., P.S. and P.C.D. were involved in the data analysis and interpretation. P.S. prepared the mutant strains. Y.-L.Y., P.S. and P.C.D. wrote the paper.

Corresponding authors

Correspondence to Paul Straight or Pieter C Dorrestein.

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Supplementary Methods and Supplementary Results (PDF 1809 kb)

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Yang, YL., Xu, Y., Straight, P. et al. Translating metabolic exchange with imaging mass spectrometry. Nat Chem Biol 5, 885–887 (2009). https://doi.org/10.1038/nchembio.252

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  • DOI: https://doi.org/10.1038/nchembio.252

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