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Enzyme catalysis

Evolution made easy

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Directed evolution is a powerful tool for the development of improved enzyme catalysts. Now, a method that enables an enzyme, its encoding DNA and a fluorescent reaction product to be encapsulated in a gel bead enables the application of directed evolution in an ultra-high-throughput format.

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Figure 1: Directed evolution of enzymes in gel-shell beads10.

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Authors and Affiliations

  1. Eugene Wee and Matt Trau are at the Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, Queensland, 4072, Australia,

    Eugene J. H. Wee

  2. Matt Trau is also at the School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland, 4072, Australia,

    Matt Trau

Authors
  1. Eugene J. H. Wee
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  2. Matt Trau
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Correspondence to Matt Trau.

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Wee, E., Trau, M. Evolution made easy. Nature Chem 6, 756–757 (2014). https://doi.org/10.1038/nchem.2046

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