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Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells

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Abstract

We have observed karyotypic changes involving the gain of chromosome 17q in three independent human embryonic stem (hES) cell lines on five independent occasions. A gain of chromosome 12 was seen occasionally. This implies that increased dosage of chromosome 17q and 12 gene(s) provides a selective advantage for the propagation of undifferentiated hES cells. These observations are instructive for the future application of hES cells in transplantation therapies in which the use of aneuploid cells could be detrimental.

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Figure 1: Karyotypic changes affecting chromosomes 17 and 12 in sublines of H7 hES cells.

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Acknowledgements

This work was supported in part by grants from the Biotechnology and Biological Sciences Research Council, Wellcome Trust and Yorkshire Cancer Research. We thank Henry Yuen and the Oskar Rennebohm Foundation for their gifts to the University of Wisconsin Foundation that helped support this work.

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Author notes

  1. Jonathan S Draper and Kath Smith: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biomedical Science, University of Sheffield, Western Bank, Sheffield, S10 2TN, UK

    Jonathan S Draper, Paul Gokhale & Peter W Andrews

  2. North Trent Clinical Cytogenetics Service, Sheffield Children's Trust, Western Bank, Sheffield, S10 2TH, UK

    Kath Smith & Edna Maltby

  3. Section of Reproductive and Developmental Medicine, University of Sheffield, Royal Hallamshire Hospital, Jessop Wing, Sheffield, S10 2SF, UK

    Harry D Moore

  4. Cytogenetic Laboratory at Wisconsin State Laboratory of Hygiene, 465 Henry Mall, Madison, 53706, Wisconsin, USA

    Julie Johnson & Lorraine Meisner

  5. National Primate Research Center and the Department of Anatomy, University of Wisconsin–Madison Medical School, 1200 Capital Court, Madison, 53715, Wisconsin, USA

    Thomas P Zwaka & James A Thomson

Authors
  1. Jonathan S Draper
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  2. Kath Smith
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  3. Paul Gokhale
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  9. James A Thomson
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  10. Peter W Andrews
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Corresponding author

Correspondence to Peter W Andrews.

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Competing interests

P.W.A. and J.S.D. have equity in Axordia, Ltd., a university spinout company dedicated to development of stem cell technology.

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Draper, J., Smith, K., Gokhale, P. et al. Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat Biotechnol 22, 53–54 (2004). https://doi.org/10.1038/nbt922

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  • DOI: https://doi.org/10.1038/nbt922

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