The specificity of a homing endonuclease has been altered using computational modeling of the protein-DNA interface.
References
Ashworth, J. et al. Nature 441, 656–659 (2006).
Stoddard, B.L. Q. Rev. Biophys. 38, 49–95 (2006).
Havranek, J.J., Duarte, C.M. & Baker, D. J. Mol. Biol. 344, 59–70 (2004).
Morozov, A.V., Havranek, J.J., Baker, D. & Siggia, E.D. Nucleic Acids Res. 33, 5781–5798 (2005).
Pabo, C.O. & Sauer, R.T. Annu. Rev. Biochem. 61, 1053–1095 (1992).
Pabo, C.O. & Nekludova, L. J. Mol. Biol. 301, 597–624 (2000).
Wolfe, S.A., Nekludova, L. & Pabo, C.O. Annu. Rev. Biophys. Biomol. Struct. 3, 183–212 (1999).
Pabo, C.O., Peisach, E. & Grant, R.A. Annu. Rev. Biochem. 70, 313–340 (2001).
Urnov, F.D. et al. Nature 435, 646–651 (2005).
Bradley, P., Misura, K.M.S. & Baker, D. Science 309, 1868–1871 (2005).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pabo, C. Specificity by design. Nat Biotechnol 24, 954–955 (2006). https://doi.org/10.1038/nbt0806-954
Issue Date:
DOI: https://doi.org/10.1038/nbt0806-954
- Springer Nature America, Inc.