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Acoustic Cell Filter for High Density Perfusion Culture of Hybridoma Cells

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Abstract

We have developed a flow–through device which uses high frequency, low energy ultrasonic resonance fields to transiently aggregate hybridoma cells and return them by sedimentation to a perfusion bioreactor. The system retained up to 99 percent of the inflowing viable cells with no measurable effect on viability. Viable cells were selectively retained at up to 3 percent higher efficiency than nonviable cells. A stirred tank bioreactor was operated for 700 hours with acoustic cell recycle. Concentrations greater than 5×107 cells/ml were attained with a 5–fold increase in antibody concentration and a 70–fold increase in volumetric productivity compared with batch culture.

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Author notes

  1. James M. Piret: Corresponding author.

Authors and Affiliations

  1. Biotechnology Laboratory & Department of Chemical Engineering, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada

    James M. Piret

  2. Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada

    Stefan A. Sonderhoff & Douglas G. Kilburn

  3. Sonofloc Biotech Inc., Richmond, BC, V6V 1Z3, Canada

    Felix Trampler, Stefan A. Sonderhoff & Phylis W. S. Pui

  4. Institute for General Physics, TechnicalUnivesity of Vienna, Vienna, A-1040, Austria

    Felix Trampler

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  1. Felix Trampler
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  2. Stefan A. Sonderhoff
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  3. Phylis W. S. Pui
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  4. Douglas G. Kilburn
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  5. James M. Piret
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Trampler, F., Sonderhoff, S., Pui, P. et al. Acoustic Cell Filter for High Density Perfusion Culture of Hybridoma Cells. Nat Biotechnol 12, 281–284 (1994). https://doi.org/10.1038/nbt0394-281

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  • DOI: https://doi.org/10.1038/nbt0394-281

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