Abstract
The anterior/posterior (A/P) and dorsal/ventral (D/V) compartment borders that subdivide the wing imaginal discs of Drosophila third instar larvae are each associated with a developmental organizer. Decapentaplegic (Dpp), a member of the transforming growth factor-β (TGF-β) superfamily, embodies the activity of the A/P organizer. It is produced at the A/P organizer and distributes in a gradient of decreasing concentration to regulate target genes, functioning non-autonomously to regulate growth and patterning of both the anterior and posterior compartments1,2,3. Wingless (Wg) is produced at the D/V organizer and embodies its activity4,5. The mechanisms that distribute Dpp and Wg are not known, but proposed mechanisms include extracellular diffusion6, successive transfers between neighbouring cells7,8, vesicle-mediated movement9, and direct transfer via cytonemes10. Cytonemes are actin-based filopodial extensions that have been found to orient towards the A/P organizer from outlying cells. Here we show that in the wing disc, cytonemes orient towards both the A/P and D/V organizers, and that their presence and orientation correlates with Dpp signalling. We also show that the Dpp receptor, Thickveins (Tkv), is present in punctae that move along cytonemes. These observations are consistent with a role for cytonemes in signal transduction.
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Acknowledgements
We gratefully acknowledge many helpful discussions with G. Ehrenkaufer, and thank K. Moses, E. Bier, L. Luo, and F. Chanut for fly stocks. This work was supported by a grant from the NIH to T.B.K.
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Supplementary information
Supplementary Tables S1 and S2
Supplementary Table S1: regional differences of cytoneme length in the wing disc. Supplementary Table S2: distribution of Tkv:GFP punctae in flattened and unflattened wing discs. (DOC 31 kb)
Supplementary Figure S1
Profiles of uncompressed and compressed wing discs. (PDF 961 kb)
Supplementary Figure Legend
Full text description of the above Supplementary Figure. (DOC 19 kb)
Supplementary Video S1
Punctae are motile, moving in both anterograde and retrograde directions and associate with cytonemes. (MOV 3527 kb)
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Hsiung, F., Ramirez-Weber, FA., David Iwaki, D. et al. Dependence of Drosophila wing imaginal disc cytonemes on Decapentaplegic. Nature 437, 560–563 (2005). https://doi.org/10.1038/nature03951
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DOI: https://doi.org/10.1038/nature03951
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