Abstract
Cytoplasmic polyhedrosis virus (CPV) is unique among the double-stranded RNA viruses of the family Reoviridae in having a single capsid layer. Analysis by cryo-electron microscopy allows comparison of the single shelled CPV and orthoreovirus with the high resolution crystal structure of the inner shell of the bluetongue virus (BTV) core. This suggests that the novel arrangement identified in BTV, of 120 protein subunits in a so-called 'T=2' organization, is a characteristic of the Reoviridae and allows us to delineate structural similarities and differences between two subgroups of the family — the turreted and the smooth-core viruses. This in turn suggests a coherent picture of the structural organization of many dsRNA viruses.
Similar content being viewed by others
References
Mertens, P.P.C. et al. Reoviridae. In Virus taxonomy. Seventh report of the international committee for the taxonomy of viruses (eds. Van Regenmortel, M.H.V. et al. ) (Academic Press, New York, San Diego; 1999).
Payne, C.C. & Mertens, P.P.C. In The Reoviridae (ed. Joklik, W.K.) 425–504 (Academic Press, New York, 1983).
Miura, K., Fujii-Kawata, I., Iwata, H. & Kawase, S. J. Invertebrate pathology 14, 262 (1969).
Yazaki, K. & Miura, K. Virology 105, 467–479 (1980).
Dryden, K.A. et al. J.Cell Biol. 122, 1023–1041 (1993).
Dryden, K.A. et al. Virology 245, 33–46 (1998).
Prasad, B.V.V., Wang, G.J., Clerx, J.P.M. & Chiu, W. J. Mol. Biol. 199, 269–275 (1988).
Schoehn, G., Moss, S.R., Nuttall, P.A. & Hewat, E.A. Virology 235, 191–200 (1997).
Prasad, B.V.V., Yamaguchi, S. & Roy, P. J.Virol. 66, 2135–2142 (1992).
Hewat, E.A., Booth, T.F. & Roy, P. J. Struct. Biol. 109, 61–69 (1992).
Shaw, A.L., Samal, S.K., Subramanian, K. & Prasad, B.V.V. Structure 4, 957–967 (1996).
Lu, G. et al. J.Virol. 72, 8541–8549 (1998).
Chen, C.C., Chen, M.J., Chiu, R.J. & Hsu, H.T. Plant Protect. Bull. Taichung 39, 383–388 (1997).
Grimes, J.M. et al. Nature 395, 470–478 (1998).
Livolant, F. & Leforestier, A. Prog. Polymer Sci. 21, 1115–1164 (1996).
Prasad, B.V.V. et al. Nature 382, 471–473 (1996).
Gouet, P. et al. Cell in the press(1999).
Cleveland, D.R., Zarbl, H. & Millward, S. J. Virol. 60, 307–311 (1986).
Metcalf, P., Cyrklaff, M. & Adrian, M. EMBO J. 10, 3129–3136 (1991).
Coombs, K.M. Virology 243, 218–228 (1998).
Bisaillon, M., Bergeron, J. & Lemay, G. J. Biol. Chem. 272, 18298–18303 (1997).
Cheng, R.H. et al. J. Mol. Biol. 244, 255–258 (1994).
Butcher, S.J., Dokland, T., Ojala, P.M., Bamford, D.H. & Fuller, S.D. EMBO J. 16, 4477–4487 (1997).
Hill, C.L., Booth, T.F., Stuart, D.I. & Mertens, P.P.C. J. Virol. Methods 78, 177–189 (1999).
Dubochet, J., Adrian, M., Lepault, J. & McDowell, A.W. Trends Biochem. Sci. 10, 143–146 (1985).
Lawton, J.A. & Prasad, B.V.V. J. Struct. Biol. 116, 209–215 (1996).
Crowther, R.A., Henderson, R. & Smith, J.M. J. Struct. Biol. 116, 9–16 (1996).
Crowther, R.A. Phil. Trans. R. Soc. Lond. B 261, 221–230 (1971).
Fuller, S.D., Butcher, S.J., Cheng, R.H. & Baker, T.S. J. Struct. Biol. 116, 48–55 (1996).
Acknowledgements
This work was supported by grants from the BBSRC and EC, an NERC industry-targeted studentship to C.L.H., sponsored by Oxford Instruments Plc. P.M. is supported by MAFF, D.I.S. by MRC and B.V.V.P. acknowledges support from the NIH. We thank T. Baker for kindly providing the electron density reconstructions for the orthoreovirus core and virion.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hill, C., Booth, T., Prasad, B. et al. The structure of a cypovirus and the functional organization of dsRNA viruses. Nat Struct Mol Biol 6, 565–568 (1999). https://doi.org/10.1038/9347
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/9347
- Springer Nature America, Inc.
This article is cited by
-
Analysis of reassortant and intragenic recombination in Cypovirus
Virology Journal (2020)
-
Tick-Borne Viruses
Virologica Sinica (2018)
-
DNA methylation in silkworm genome may provide insights into epigenetic regulation of response to Bombyx mori cypovirus infection
Scientific Reports (2017)
-
Molecular insights into RNA-binding properties of Escherichia coli–expressed RNA-dependent RNA polymerase of Antheraea mylitta cytoplasmic polyhedrosis virus
Archives of Virology (2017)
-
The infectious particle of insect-borne totivirus-like Omono River virus has raised ridges and lacks fibre complexes
Scientific Reports (2016)