Abstract
THE intracellular protein tyrosine kinase FAK (focal adhesion kinase) was originally identified by its high level of tyrosine phosphorylation in v-src-transformed cells1á¤-4. FAK is also highly phosphorylated during early development5,6. In cultured cells it is localized to focal adhesion contacts and becomes phosphorylated and activated in response to integrin-mediated binding of cells to the extracellular matrix, suggesting an important role in cell adhesion and/or migration. We have generated FAK-deficient mice by gene targeting to examine the role of FAK during development. Mutant embryos displayed a general defect of mesoderm development, and cells from these embryos had reduced mobilityin vitro. Surprisingly, the number of focal adhesions was increased in FAK-deficient cells, suggesting that FAK may be involved in the turnover of focal adhesion contacts during cell migration.
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llić, D., Furuta, Y., Kanazawa, S. et al. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature 377, 539–544 (1995). https://doi.org/10.1038/377539a0
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DOI: https://doi.org/10.1038/377539a0
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