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Identification of self peptides bound to purified HLA-B27

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Abstract

A pool of endogenous peptides bound to the human class I MHC molecule, HLA-B27, has been isolated. Microsequence analysis of the pool and of 11 HPLC-purified peptides provides information on the binding specificity of the HLA-B27 molecule. The peptides all seem to be nonamers, seven of which match to protein sequences in a database search. These self peptides derive from abundant cytosolic or nuclear proteins, such as histone, ribosomal proteins, and members of the 90K heat-shock protein family.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    T. S. Jardetzky, D. R. Madden & D. C. Wiley

  2. Howard Hughes Medical Institute, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    D. C. Wiley

  3. Committee on Higher Degrees in Biophysics, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    D. R. Madden

  4. Harvard Microchemistry Facility, 16 Divinity Avenue, Cambridge, Massachusetts, 02138, USA

    W. S. Lane & R. A. Robinson

Authors
  1. T. S. Jardetzky
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  2. W. S. Lane
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  3. R. A. Robinson
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  4. D. R. Madden
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  5. D. C. Wiley
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Jardetzky, T., Lane, W., Robinson, R. et al. Identification of self peptides bound to purified HLA-B27. Nature 353, 326–329 (1991). https://doi.org/10.1038/353326a0

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  • DOI: https://doi.org/10.1038/353326a0

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