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Cloning and expression of a cDNA encoding an endothelin receptor

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Abstract

ENDOTHELINS are a newly described peptide family consisting of three peptides (ET-1, ET-2 and ET-3) which are the most potent vasoconstrictive peptides known1–3. They are crucial in the regulation of vascular smooth muscle tone1–3. The diverse functions of endothelins are thought to be mediated by interaction with many different receptors coupled to the inositol phosphate/calcium ion messenger pathway. However, because of the structural resemblance of the three peptides, the presence and nature of multiple endothelin receptors remain to be elucidated. We report here the cloning of a complementary DNA encoding a bovine endothelin receptor, which has a transmembrane topology similar to that of other G protein-coupled receptors and shows specific binding, with the highest selectivity to ET-1 in animal cells trans-fected with the cloned cDNA. This receptor messenger RNA is widely distributed in the central nervous system and peripheral tissues, particularly in the heart and lung. Our results support the view that there are other receptor subtypes.

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Author notes

  1. Shigetada Nakanishi: To whom correspondence should be addressed.

Authors and Affiliations

  1. Institute for Immunology, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Hiroshi Arai, Seiji Hori, Ichiro Aramori, Hiroaki Ohkubo & Shigetada Nakanishi

Authors
  1. Hiroshi Arai
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  2. Seiji Hori
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  3. Ichiro Aramori
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  4. Hiroaki Ohkubo
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  5. Shigetada Nakanishi
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Arai, H., Hori, S., Aramori, I. et al. Cloning and expression of a cDNA encoding an endothelin receptor. Nature 348, 730–732 (1990). https://doi.org/10.1038/348730a0

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  • DOI: https://doi.org/10.1038/348730a0

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