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Cloning and expression of human and rat Dt dopamine receptors

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Abstract

THE importance of the dopaminergic system in brain function has been emphasized by its association with neurological and psychiatric disorders such as Parkinson's disease and schizophrenia. On the basis of their biochemical and pharmacological characteristics, dopamine receptors are classified into D1 and D2 subtypes1,2. As the most abundant dopamine receptor in the central nervous system, D1 receptors seem to mediate some behavioural responses3, modulate activity of D2 dopamine receptors4,5, and regulate neuron growth and differentiation6. The D2 dopamine receptor has been cloned by low-stringency screening7. We report here the cloning of human and rat D1 dopamine receptors by applying an approach based on the polymerase chain reaction8. The cloned human D1 dopamine receptor has been characterized on the basis of four criteria: the deduced amino-acid sequence, which reveals that it is a G protein-coupled receptor; the tissue distribution of its messenger RNA, which is compatible with that of the Dl dopamine receptor; its pharmacological profile when transfected into COS-7 cells; and its ability to stimulate the accumulation of cyclic AMP in human 293 cells.

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References

  1. Kebabian, J. W. & Calne, D. B. Nature 277, 93–96 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Creese, I., Sibley, D. R., Hamblin, M. W. & Leff, S. E. A. Rev. Neurosci. 6, 43–71 (1983).

    Article  CAS  Google Scholar 

  3. Clark, D. & White, F. J. Synapse 1, P347–P388 (1987).

    Article  Google Scholar 

  4. Walters, J. R., Bergstrom, D. A., Carlson, J. H., Chase, T. N. & Braun, A. R. Science 236, 719–722 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Waddington, J. L. J. Psychopharmacol. 3, 54–63 (1989).

    Article  CAS  PubMed  Google Scholar 

  6. Lankford, K. L., DeMello, F. G. & Klein, W. L. Proc. natn. Acad. Sci. U.S.A. 85, 4567–4571 (1988).

    Article  ADS  CAS  Google Scholar 

  7. Bunzow, J. R. et al. Nature 336, 783–787 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Saiki, R. K. et al. Science 239, 487–491 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Dohlman, H. G., Caron, M. G. & Lefkowitz, R. J. Biochemistry 26, 2657–2664 (1987).

    Article  CAS  PubMed  Google Scholar 

  10. Libert, F. et al. Science 244, 569–572 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Boyson, S. J., McGonigle, P. & Molinoff, P. B. J. Neurosci. 6, 3177–3188 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. O'Dowd, B. F. et al. J. biol. Chem. 263, 15985–15992 (1988).

    CAS  PubMed  Google Scholar 

  13. Emorine, L. J. et al. Science 245, 1118–1121 (1989).

    Article  ADS  CAS  PubMed  Google Scholar 

  14. Regan, J. W. et al. Proc. natn. Acad. Sci. U.S.A. 85, 6301–6305 (1988).

    Article  ADS  CAS  Google Scholar 

  15. Strader, C. D., Candelore, M. R., Hill, W. S., Sigal, I. S. & Dixon, R. A. F. J. biol. Chem. 264, 13572–13578 (1989).

    CAS  PubMed  Google Scholar 

  16. Lefkowitz, R. J. et al. Cold Spring Harb. Symp. quant. Biol. 53, 507–514 (1988).

    Article  CAS  PubMed  Google Scholar 

  17. Jarvie, K. R., Booth, G., Brown, E. M. & Niznik, H. B. Molec. Pharmacol. 36, 566–574 (1989).

    CAS  Google Scholar 

  18. Grandy, D. K. et al. Proc. natn Acad. Sci. U.S.A. 86, 9762–9766 (1989).

    Article  ADS  CAS  Google Scholar 

  19. Kobilka, B. K. et al. Proc. natn Acad. Sci. U.S.A. 84, 46–50 (1987).

    Article  ADS  CAS  Google Scholar 

  20. Frielle, T. et al. Proc. natn. Acad. Sci. U.S.A. 84, 7920–7924 (1987).

    Article  ADS  CAS  Google Scholar 

  21. Cotecchia, S. et al. Proc. natn. Acad. Sci. U.S.A. 85, 7159–7163 (1988).

    Article  ADS  CAS  Google Scholar 

  22. Kobilka, B. K. et al. Science 238, 650–656 (1987).

    Article  ADS  CAS  PubMed  Google Scholar 

  23. Strader, C. D. et al. J. biol. Chem. 263, 10267–10271 (1988).

    CAS  PubMed  Google Scholar 

  24. Venter, J. C., Fraser, C. M., Kerlavage, A. R. & Buck, M. Biochem. Pharmacol. 38, 1197–1208 (1989).

    Article  CAS  PubMed  Google Scholar 

  25. Kishimoto, A. et al. J. biol. Chem. 260, 12492–12499 (1985).

    CAS  PubMed  Google Scholar 

  26. Bouvier, M. et al. Nature 333, 370–374 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  27. Cullen, B. R. Meth. Enzym. 152, 684–704 (1987).

    Article  CAS  PubMed  Google Scholar 

  28. Niznik, H. B. et al. Molec. Pharmacol. 34, 29–36 (1990).

    Google Scholar 

  29. Chen, C. & Okayama, H. Molec. cell. Biol. 7, 2745–2752 (1987).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Brown, B. L., Albano, J. D., Ekins, R. P. & Sgherzi, A. M. Biochem. J. 171, 561–562 (1971).

    Article  Google Scholar 

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Author notes

  1. Hubert H. M. Van Tol

    Present address: Department of Pharmacology, Medical Sciences Building, University of Toronto, Toronto, Ontario, M5S 1A8, Canada

Authors and Affiliations

  1. Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA

    Qun-Yong Zhou, David K. Grandy, Lisa Thambi, Jake A. Kushner, Hubert H. M. Van Tol, Roger Cone, John Salon, James R. Bunzow & Olivier Civelli

  2. Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA

    Qun-Yong Zhou

  3. Department of Pharmacology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA

    Lisa Thambi

  4. Department of Cell Biology and Anatomy, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201, USA

    David Pribnow & Olivier Civelli

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  1. Qun-Yong Zhou
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Zhou, QY., Grandy, D., Thambi, L. et al. Cloning and expression of human and rat Dt dopamine receptors . Nature 347, 76–80 (1990). https://doi.org/10.1038/347076a0

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  • DOI: https://doi.org/10.1038/347076a0

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