Abstract
Actively transcribing eukaryotic RNA polymerase II is highly phosphorylated on its repetitive carboxyl-terminal domain. We have isolated a protein kinase that phosphorylates serine residues in this repetitive domain. A component of this kinase is cdc2, the product of a cell-cycle control gene previously shown to be a component of M-phase-promoting factor and M-phase-specific histone H1 kinase. This observation suggests a role for the cdc2 protein kinase in transcriptional regulation.
Similar content being viewed by others
References
Corden, J. L., Cadena, D. L., Ahearn, J. M. & Dahmus, M. E. Proc. natn. Acad. Sci. U.S.A. 82, 7934–7938 (1985).
Allison, L. A., Wong, J. K-C., Fitzpatrick, V. D., Moyle, M. & Ingles, C. J. Molec. cell. Biol. 8, 321–329 (1988).
Zering, W. A., Lee, J. M., Weeks, J. R., Jokerst, R. S. & Greenleaf, A. L. Proc. natn. Acad. Sci. U.S.A. 85, 3698–3702 (1988).
Allison, L. A., Moyle, M., Shales, M. & Ingles, C. J. Cell 42, 599–610 (1985).
Nonent, M., Sweetser, D. & Young, Y. A. Cell 50, 909–915 (1987).
Bartolomei, M. S., Halden, N. F., Cullen, C. R. & Corden, J. L. Molec. cell. Biol. 8, 330–339 (1988).
Bartholomew, B., Dahmus, M. E. & Meares, C. F. J. biol. Chem. 261, 14226–14231 (1986).
Cadena, D. L. & Dahmus, M. E. J. biol. Chem. 262, 12468–12474 (1987).
Ahearn, J. M., Bartolomei, M. S., West, M. L., Cisek, L. J. & Corden, J. L. J. biol. Chem. 262, 10695–10705 (1987).
Christman, J. L. & Dahmus, M. E. J. biol. Chem. 256, 11798–11803 (1981).
Guilfoyle, T. J., Hagen, G. & Malcolm, S. J. biol. Chem. 259, 649–653 (1984).
Kim, W. & Dahmus, M. E. J. biol. Chem. 261, 14219–14225 (1986).
Dahmus, M. E. J. biol. Chem. 256, 3332–3339 (1981).
Kuenzel, E. A. & Krebs, E. G. Proc. natn. Acad. Sci. U.S.A. 82, 737–741 (1985).
Abersold, R. H. et al. Proc. natn. Acad. Sci. U.S.A. 84, 6970–6974 (1987).
Lee, M. & Nurse, P. Nature 327, 31–35 (1987).
Lorincz, A. & Reed, S. Nature 307, 183–185 (1984).
Hindley, J. & Phear, G. Gene 31, 129–134 (1984).
Hanks, S. K. Proc. natn. Acad. Sci. U.S.A. 84, 388–392 (1987).
Nurse, P. Trends Genet. 1, 51–55 (1985).
Draetta, G. & Beach, D. Cell 54, 17–26 (1988).
Hartwell, L. H. Bact. Rev. 38, 165–198 (1974).
Beach, D., Durkacz, B. & Nurse, P. Nature 300, 706–709 (1981).
Nurse, P. & Bissett, Y. Nature 292, 558–560 (1981).
Wittenberg, C. & Reed, S. I. Cell 54, 1061–1072 (1988).
Draetta, G. et al. Cell 56, 829–838 (1989).
Solomon, M., Booher, R., Kirschner, M. & Beach, D. Cell 54, 738–740 (1988).
Goebl, M. & Byers, B. Cell 54, 738–740 (1988).
Gautier, J., Norbury, C. Lohka, M., Nurse, P. & Maller, J. Cell 54, 433–439 (1988).
Dunphy, W. G., Brizuela, L., Beach, D. & Newport, J. Cell 54, 423–431 (1988).
Arion, D., Meijer, L., Brizuela, L. & Beach, D. Cell 55, 371–378 (1988).
Labbe, J. C. Lee, M. G., Nurse, P., Picard, A. & Dorre, M. Nature 335, 251–254 (1988).
Draetta, G. et al. Nature 336, 738–744 (1988).
Sigler, P. S. Nature 333, 210–212 (1988).
Bunick, D., Zandomeni, R., Ackerman, S. & Weinmann, R. Cell 29, 877–886 (1982).
Sawadogo, M. & Roeder, R. G. J. biol. Chem. 259, 5321–5326 (1984).
Prescott, D. M. & Bender, M. A. Expl Cell Res. 26, 260–268 (1962).
Arnt, K. T., Styles, C. A. & Fink, G. R. Cell 56, 527–537 (1989).
Manley, J. L. Proc. natn. Acad. Sci. U.S.A. 77, 3855–3859 (1980).
Laemmli, U. K. Nature 227, 680–683 (1970).
Morrissey, J. H. Analyt. Biochem. 117, 307–310 (1981).
Hodo, H. G. & Blatti, S. P. Biochemistry 16, 2334–2343 (1977).
Bylund, D. B. & Huang, T. S. Analyt. Biochem. 73, 477–485 (1975).
Hunter, T. & Sefton, B. M. Proc. natn. Acad. Sci. U.S.A. 77, 1311–1315 (1979).
Hibbs, M. L., Classon, B. J., Walker, J. D., McKerzie, J. F. C. & Hogarth, P. M. J. Immun. 140, 544–550 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cisek, L., Corden, J. Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein cdc2. Nature 339, 679–684 (1989). https://doi.org/10.1038/339679a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/339679a0
- Springer Nature Limited
This article is cited by
-
Structure and Basal Transcription Complex of RNA Polymerase II Core Promoters in the Mammalian Genome: An Overview
Molecular Biotechnology (2010)
-
CDKA and CDKB kinases from Chlamydomonas reinhardtii are able to complement cdc28 temperature-sensitive mutants of Saccharomyces cerevisiae
Protoplasma (2008)
-
Cyclin C/CDK8 and cyclin H/CDK7/p36 are biochemically distinct CTD kinases
Oncogene (1999)
-
Cdk-activating kinase complex is a component of human transcription factor TFIIH
Nature (1995)