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Expression of a polypeptide containing a dipeptide repeat is confined to the insect stage of Trypanosoma brucei

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Abstract

The protozoan parasite Trypanosoma brucei is transmitted between mammalian hosts by the tsetse fly (Glossina spp.). Trypanosomes ingested by the fly undergo a number of changes in the insect midgut during differentiation to procyclic forms. These include the loss of the variant specific glycoprotein (VSG) coat1 and the appearance of a common set of procyclic surface antigens2. In order to investigate genes other than VSG genes which are expressed only at certain stages of the life cycle, the first cDNA specific to procyclic culture form trypanosomes (equivalent to the stage found in the insect midgut) has been characterized. The encoded polypeptide shows several characteristics of membrane proteins, but its most striking feature is the presence of a repetitive amino-acid sequence in which there are 22 tandem repeats of the dipeptide -Glu-Pro-. Related genes are also found in other trypanosome species and in leishmania. This gene shows many similarities to a number of surface antigen genes described in malaria3 and, more recently, Trypanosoma cruzi4. This is the first example of a repetitive sequence in a parasite protein which is present only in the insect vector, and which therefore cannot be implicated in the mammalian host immune response4–6.

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References

  1. Barry, J. D. & Vickerman, K. Expl Parasit. 48, 313–324 (1979).

    Article  CAS  Google Scholar 

  2. Seed, J. R. Parasitology 54, 593–596 (1964).

    Article  CAS  Google Scholar 

  3. Kemp, D. J. et al. Parasitology 91, S83–S108 (1986).

    Article  Google Scholar 

  4. Peterson, D. S., Wrightsman, R. A. & Manning, J. E. Nature 322, 566–568 (1986).

    Article  ADS  CAS  Google Scholar 

  5. Ravetch, J. V., Kochan, J. & Perkins, M. Science 227, 1593–1597 (1985).

    Article  ADS  CAS  Google Scholar 

  6. Berzins, K. et al. Proc. natn. Acad. Sci. U.S.A. 83, 1065–1069 (1986).

    Article  ADS  CAS  Google Scholar 

  7. Cross, G. A. M. & Manning, J. C. Parasitology 67, 315–331 (1973).

    Article  CAS  Google Scholar 

  8. Van der Ploeg, L. H. T., Gianni, S. H. & Cantor, C. R. Science 228, 1443–1445 (1985).

    Article  ADS  CAS  Google Scholar 

  9. De Lange, T., Michels, P. A. M., Veerman, H. J. G., Cornelissen, A. W. C A. & Borst, P. Nucleic Acids Res. 12, 3777–3790 (1984).

    Article  CAS  Google Scholar 

  10. Parsons, M., Nelson, R. G., Watkins, K. P. & Agabian, N. Cell 38, 309–316 (1984).

    Article  CAS  Google Scholar 

  11. Clayton, C. E. EMBO J. 4, 2997–3003 (1985).

    Article  MathSciNet  CAS  Google Scholar 

  12. Boothroyd, J. C., Paynter, C. A., Cross, G. A. M., Bernards, A. & Borst, P. Nucleic Acids Res. 9, 4735–4743 (1981).

    Article  CAS  Google Scholar 

  13. Kozak, M. Cell 44, 283–292 (1986).

    Article  CAS  Google Scholar 

  14. Blobel, G. & Dobberstein, B. J. cell Biol. 67, 835–851 (1975).

    Article  CAS  Google Scholar 

  15. Porter, A. G. et al. Nature 282, 471–477 (1979).

    Article  ADS  CAS  Google Scholar 

  16. Ward, C. W. & Dopheide, T. A. Br. med. Bull. 35, 51–56 (1979).

    Article  CAS  Google Scholar 

  17. Williams, R. O., Young, J. R. & Majiwa, P. A. O. Nature 299, 417–421 (1982).

    Article  ADS  CAS  Google Scholar 

  18. De Lange, T. & Borst, P. Nature 299, 451–453 (1982).

    Article  ADS  CAS  Google Scholar 

  19. Van der Ploeg, L. H. T., Schwartz, D. C., Cantor, C. R. & Borst, P. Cell 37, 77–84 (1984).

    Article  CAS  Google Scholar 

  20. Miller, E. N. & Turner, M. J. Parasitology 82, 63–80 (1981).

    Article  CAS  Google Scholar 

  21. Cully, D. F., Ip, H. S. & Cross, G. A. M. Cell 42, 173–182 (1985).

    Article  CAS  Google Scholar 

  22. Gibson, W. C., Osinga, K. A., Michels, P. A. M. & Borst, P. Molec. biochem. Parasit. 16, 231–242 (1985).

    Article  CAS  Google Scholar 

  23. Muskavitch, M. A. T. & Hogness, D. S. Cell 29, 1041–1051 (1982).

    Article  CAS  Google Scholar 

  24. Garfinkel, M. D., Pruitt, R. E. & Meyerowitz, E. M. J. molec. Biol. 168, 765–789 (1983).

    Article  CAS  Google Scholar 

  25. McMaster, G. K. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835–4838 (1977).

    Article  ADS  CAS  Google Scholar 

  26. Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1977).

    Article  CAS  Google Scholar 

  27. Carrington, D. M., Auffret, A. D. & Hanke, D. E. Nature 313, 64–67 (1985).

    Article  ADS  CAS  Google Scholar 

  28. Caton, A. J. & Robertson, J. S. Nucleic Acids Res. 7, 1445–1456 (1979).

    Article  CAS  Google Scholar 

  29. Watson, C. J. & Jackson, J. F. in DNA Cloning: A Practical Approach Vol. 1 (ed. Glover, D.) 79–88 (IRL, Oxford, 1985).

    Google Scholar 

  30. Murray, N. in Lambda II (eds Hendrix, R. W. et al.) 395–432 (Cold Spring Harbor Laboratory, New York, 1983).

    Google Scholar 

  31. Grunstein, M. & Hogness, D. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).

    Article  ADS  CAS  Google Scholar 

  32. Feinberg, A. P. & Vogelstein, B. Analyt. Biochem. 132, 6–13 (1983).

    Article  CAS  Google Scholar 

  33. Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  34. Vieira, J. & Messing, J. Gene 19, 259–268 (1982).

    Article  CAS  Google Scholar 

  35. Messing, J. Meth. Enzym. 101, 20–77 (1983).

    Article  CAS  Google Scholar 

  36. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  37. Young, J. R., Donelson, J. E., Majiwa, P. A. O., Shapiro, S. Z. & Williams, R. O. Nucleic Acids Res. 10, 803–819 (1982).

    Article  CAS  Google Scholar 

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Author notes

  1. Isabel Roditi & Mark Carrington

    Present address: Institut für Genetik, Kernforschungszentrum, Postfach 3640, 7500, Karlsruhe, 1, FRG

  2. Mervyn Turner

    Present address: Merck, Sharp & Dohme Reseach Laboratories, PO Box 2000, Rahway, New Jersey, 07065-0900, USA

Authors and Affiliations

  1. MRC Biochemical Parasitology Unit, Molteno Institute, Downing Street, Cambridge, CB2 3EE, UK

    Isabel Roditi, Mark Carrington & Mervyn Turner

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  1. Isabel Roditi
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  2. Mark Carrington
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  3. Mervyn Turner
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Roditi, I., Carrington, M. & Turner, M. Expression of a polypeptide containing a dipeptide repeat is confined to the insect stage of Trypanosoma brucei. Nature 325, 272–274 (1987). https://doi.org/10.1038/325272a0

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