Abstract
The Torpedo and calf acetylcholine receptors and hybrids composed of subunits from the two species have been produced in Xenopus oocytes by the use of the cloned complementary DNAs. Single-channel current measurements indicate that these receptors form channels of similar conductance but with different gating behaviour.
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Katz, B. & Miledi, R. J. Physiol., Lond. 224, 665–699 (1972).
Sakmann, B. & Neher, E. A. Rev.Physiol. 46, 455–472 (1984).
Karlin, A. Cell Surface Rev. 6, 191–260 (1980).
Changeux, J.-P. Harvey Lect. 75, 85–254 (1981).
Conti-Tronconi, B. M. & Raftery, M. A. A. Rev. Biochem. 51, 491–530 (1982).
Noda, M. et al. Nature 299, 793–797 (1982).
Noda, M. et al. Nature 301, 251–255 (1983).
Noda, M. et al. Nature 302, 528–532 (1983).
Claudio, T., Ballivet, M., Patrick, J. & Heinemann, S. Proc. natn. Acad. Sci. U.S.A. 80, 1111–1115 (1983).
Sumikawa, K. et al. Nucleic Acids Res. 10, 5809–5822 (1982).
Devillers-Thiery, A., Giraudat, J., Bentaboulet, M. & Changeux, J.-P. Proc. natn. Acad. Sci U.S.A. 80, 2067–2071 (1983).
Noda, M. et al. Nature 305, 818–823 (1983).
Tanabe, T. et al. Eur. J. Biochem. 144, 11–17 (1984).
Takai, T. et al. Eur. J. Biochem. 143, 109–115 (1984).
Kubo, T. et al. Eur. J. Biochem. 149, 5–13 (1985).
Shibahara, S. et al. Eur. J. Biochem. 146, 15–22 (1985).
LaPolla, R. J., Mayne, K. M. & Davidson, N. Proc. natn. Acad. Sci. U.S.A. 81, 7970–7974 (1984).
Nef, P., Mauron, A., Stalder, R., Alliod, C. & Ballivet, M. Proc. natn. Acad. Sci. U.S.A. 81, 7975–7979 (1984).
Takai, T. et al. Nature 315, 761–764 (1985).
Mishina, M. et al. Nature 307, 604–608 (1984).
Mishina, M. et al. Nature 313, 364–369 (1985).
Green, M. R., Maniatis, T. & Melton, D. A. Cell 32, 681–694 (1983).
Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
Neher, E. & Sakmann, B. Proc. natn. Acad. Sci. U.S.A. 72, 2140–2144 (1975).
Adams, P. R. & Sakmann, B. J. Physiol., Lond. 283, 621–644 (1978).
Colquhoun, D. in Drug Receptors (ed. Rang. H. P.) 149–182 (Macmillan, London, 1973).
Eisenman, G. Biophys. J. 2, Suppl. 2, 259–323 (1962).
Guy, H. R. Biophys. J. 45, 249–261 (1984).
Finer-Moore, J. & Stroud, R. M. Proc. natn. Acad. Sci. U.S.A. 81, 155–159 (1984).
Colquhoun, D. & Sakmann, B. J. Physiol., Lond. (in the press).
Colquhoun, D. & Sakmann, B. Nature 294, 464–466 (1981).
Sytkowski, A. J., Vogel, Z. & Nirenberg, M. W. Proc. natn. Acad. Sci. U.S.A. 70, 270–274 (1973).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sei. U.S.A 74, 5463–5467 (1977).
Gurdon, J. B. The Control of Gene Expression in Animal Development (Clarendon, Oxford, 1974).
Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers Arch. ges. Physiol. 391, 85–100 (1981).
Colquhoun, D. & Sigworth, F.J. in Single Channel Recording (eds Sakmann, B. & Neher, E.) 191–263 (Plenum, New York, 1983).
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Sakmann, B., Methfessel, C., Mishina, M. et al. Role of acetylcholine receptor subunits in gating of the channel. Nature 318, 538–543 (1985). https://doi.org/10.1038/318538a0
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DOI: https://doi.org/10.1038/318538a0
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