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Novel immunoglobulin heavy chains are produced from DJH gene segment rearrangements in lymphoid cells

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Abstract

It has been found that most immunoglobulin heavy(H)-chain gene diversity (D) segments carry their own 5′ transcriptional promoter element. Transcription of rearrangements which use heavy-chain D and joining (J) segments in B-lymphoid cells ultimately leads to the production of a Dμ messenger RNA which contains the DJH segments linked to the μ heavy-chain constant region. If the D is joined to the JH segment in the appropriate translational reading frame, this Dμ mRNA is translated to yield a short Dμ protein with a variable DJH N-terminus.

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References

  1. Early, P., Huang, H., Davis, M., Calame, K. & Hood, L. Cell 19, 981–992 (1980).

    Article  CAS  Google Scholar 

  2. Sakano, H., Kurosawa, Y., Weigert, M. & Tonegawa, S. Nature 290, 562–565 (1981).

    Article  ADS  CAS  Google Scholar 

  3. Kurosawa, Y. et al. Nature 290, 565–570 (1981).

    Article  ADS  CAS  Google Scholar 

  4. Alt, F. W. et al. EMBO J. 3, 1209–1219 (1984).

    Article  CAS  Google Scholar 

  5. Kabat, E., Wu, T. T., Bilofsky, H., Reid-Miller, M. & Perry, H. (eds) Sequences of Proteins of Immunological Interest No. 80-2008 (NIH, Bethesda, 1983).

  6. Wood, C. & Tonegawa, S. Proc. natn. Acad. Sci. U.S.A. 80, 3030–3034 (1983).

    Article  ADS  CAS  Google Scholar 

  7. Kurosawa, Y. & Tonegawa, S. J. exp. Med. 155, 201–208 (1981).

    Article  Google Scholar 

  8. Alt, F. W., Rosenberg, N., Lewis, S., Thomas, E. & Baltimore, D. Cell 27, 381–390 (1981).

    Article  CAS  Google Scholar 

  9. Alt, F. W., Rosenberg, N., Enea, V., Siden, E. & Baltimore, D. Molec. cell. Biol. 2, 386–400 (1982).

    Article  CAS  Google Scholar 

  10. Towbin, H., Staehlin, T. & Gorden, J. Proc. natn. Acad. Sci. U.S.A. 76, 4350–4356 (1979).

    Article  ADS  CAS  Google Scholar 

  11. Alt, F. W. et al. Cell 20, 283–291 (1980).

    Article  Google Scholar 

  12. Early, P. et al. Cell 20, 313–320 (1980).

    Article  CAS  Google Scholar 

  13. Blackwell, T. K. & Alt, F. W. Cell 37, 105–112 (1984).

    Article  CAS  Google Scholar 

  14. Lewis, S., Gilford, A. & Baltimore, D. Nature 308, 425–428 (1984).

    Article  ADS  CAS  Google Scholar 

  15. Alt, F. W. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 79, 4118–4122 (1982).

    Article  ADS  CAS  Google Scholar 

  16. Nelson, K., Haimovich, J. & Perry, R. P. Molec. cell. Biol. 3, 1317–1332 (1983).

    Article  CAS  Google Scholar 

  17. Kozak, M. Nature 308, 241–246 (1984).

    Article  ADS  CAS  Google Scholar 

  18. Cosy, S., Adams, J. M. & Kemp, D. J. Proc. natn. Acad. Sci. U.S.A. 77, 4943–4947 (1980).

    Article  ADS  Google Scholar 

  19. Zuniga, M. C., D'Eustachio, P. & Ruddle, N. H. Proc. natn. Acad. Sci. U.S.A. 79, 3015–3019 (1982).

    Article  ADS  CAS  Google Scholar 

  20. Forster, A., Hobart, M., Hengartner, H. & Rabbitts, T. H. Nature 286, 897–899 (1980).

    Article  ADS  CAS  Google Scholar 

  21. Chang, T. W. & Eisen, H. N. Nature 280, 406–408 (1980).

    Article  ADS  Google Scholar 

  22. Kemp, D. J., Wilson, A., Harris, A. W. & Shortman, K. Nature 286, 168–170 (1980).

    Article  ADS  CAS  Google Scholar 

  23. Kemp, D. J., Harris, A. W., Cory, S. & Adams, J. M. Proc. natn. Acad. Sci. U.S.A. 77, 2876–2880 (1980).

    Article  ADS  CAS  Google Scholar 

  24. Gillies, S. D., Morrison, S. L., Oi, V. T. & Tonegawa, S. Cell 33, 717–728 (1983).

    Article  CAS  Google Scholar 

  25. Banerji, J., Olson, L. & Schaffner, W. Cell 33, 729–740 (1983).

    Article  CAS  Google Scholar 

  26. Kabat, A. E., Wu, T. T. & Bilofsky, H. J. exp. Med. 149, 1299–1313 (1979).

    Article  CAS  Google Scholar 

  27. Bothwell, A. L. et al. Cell 24, 625 (1981).

    Article  CAS  Google Scholar 

  28. Szenberg, A., Marchalonis, J. J. & Warner, N. L. Proc. natn. Acad. Sci. U.S.A. 74, 2113–2117 (1975).

    Article  ADS  Google Scholar 

  29. Jensenius, J. C. & Williams, A. F. Nature 300, 583–588 (1982).

    Article  ADS  CAS  Google Scholar 

  30. Rajewsky, K. & Eichman, K. Contemp. Topics Immun. 7, 69–112 (1977).

    Article  CAS  Google Scholar 

  31. Ravetch, J. V., Siebenlist, U., Korsmeyer, S., Waldmann, T. & Leder, P. Cell 27, 583–591 (1981).

    Article  CAS  Google Scholar 

  32. Siu, G. et al. Nature 311, 344–350 (1984).

    Article  ADS  CAS  Google Scholar 

  33. Desiderio, S. V. & Baltimore, D. Nature 308, 860–862 (1984).

    Article  ADS  CAS  Google Scholar 

  34. Crawford, S. & Goff, S. P. J. Virol. 49, 909–917 (1984).

    CAS  PubMed  PubMed Central  Google Scholar 

  35. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  36. Reth, M., Haemmerling, G. J. & Rajewsky, K. Eur. J. Immun. 8, 393–400 (1978).

    Article  CAS  Google Scholar 

  37. Maxam, A. & Gilbert, W. Meth. Enzym. 65, 419–460 (1980).

    Google Scholar 

  38. Allan, M., Lanyon, W. G. & Paul, J. Cell 35, 187–197 (1983).

    Article  CAS  Google Scholar 

  39. Lonberg, N. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 80, 3661–3665 (1983).

    Article  ADS  CAS  Google Scholar 

  40. Neuberger, M. S. EMBO J. 2, 1373–1378 (1983).

    Article  CAS  Google Scholar 

  41. Mercola, M., Wang, X., Olsen, J. & Calame, K. Science 221, 663–665 (1983).

    Article  ADS  CAS  Google Scholar 

  42. Schwaber, J., Molgaard, H., Orkin, S. H., Gould, H. J. & Rosen, F. S. Nature 304, 355–358 (1983).

    Article  ADS  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Institute for Cancer Research, Columbia University, College of Physicians and Surgeons, New York, 10032, USA

    Michael G. Reth & Frederick W. Alt

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  1. Michael G. Reth
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  2. Frederick W. Alt
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Reth, M., Alt, F. Novel immunoglobulin heavy chains are produced from DJH gene segment rearrangements in lymphoid cells. Nature 312, 418–423 (1984). https://doi.org/10.1038/312418a0

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  • DOI: https://doi.org/10.1038/312418a0

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