Abstract
Differentiation of B lymphocytes follows an ordered pathway marked by somatic rearrangements of immunoglobulin heavy- and light-chain genes in conjunction with cellular transitions1–4. Although low level constitutive transcription of the μ heavy-chain constant region (Cμ) genes may occur in early precursor cells5, activation of synthesis and translation of complete μ RNA is thought to accompany somatic rearrangements of DNA6. Cytoplasmic μ-chain protein serves as a marker for pre-B cells, the earliest cells committed to differentiation into B lymphocytes4,7,8. μ-chain gene expression in pre-B cells precedes rearrangement and expression of light-chain genes6,8. We now report that early human pre-B cells, Epstein–Barr virus transformed pre-B cells, and pre-B cell hybrid analogues, produce Cμ without the normally associated heavy-chain variable (VH) region. Approximately 5% of normal pre-B cells from adult human bone marrow produce these incomplete μ-chains. Pre-B cells from three patients with X-linked agammaglobulinaemia are exclusively of this immature form, producing Cμ without associated VH. This immune deficiency disease represents a block in differentiation secondary to failure to express VH genes.
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Schwaber, J., Molgaard, H., Orkin, S. et al. Early pre-B cells from normal and X-linked agammaglobulinaemia produce Cμ without an attached VH region. Nature 304, 355–358 (1983). https://doi.org/10.1038/304355a0
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DOI: https://doi.org/10.1038/304355a0
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