Abstract
Neuropeptides are frequently found in neurones that also contain another putative transmitter substance such as a biogenic amine or acetylcholine1–5. Such co-existence has led to the suggestion that the roles of neuropeptides in the nervous system may be diverse and may in some systems include a part in conventional neurotransmission as well as an ancillary, or modulatory, role. Recently, several studies have noted the co-occurrence of two peptides within individual neurones of localized regions of the mammalian nervous system6–9. Here, we present the first evidence, based on an indirect immunofluorescence technique allowing the simultaneous visualization of separate tissue antigens, that substance P (SP) and Leu-enkephalin (L-Enk) co-occur within individual neurones of widespread regions of the avian central nervous system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chan-Palay, V., Jonsson, G. & Palay, S. L. Proc. natn. Acad. Sci. U.S.A. 75, 1582–1586 (1978).
Hokfelt, T. et al. Neuroscience 3, 517–538 (1978).
Hokfelt, T. et al. Nature 285, 476–478 (1980).
Hokfelt, T. et al. Neuroscience 5, 2093–2124 (1980).
Lundberg, J. M. et al. Neuroscience 4, 1539–1559 (1979).
Watson, S. J., Akil, H., Richard, C. W. & Barchas, J. D. Nature 275, 226–228 (1978).
Schultzberg, M. et al. Neuroscience 5, 689–744 (1980).
Hokfelt, T. et al. in Regulation and Function of Neuronal Peptides (eds Trabucchi, M. & Costa, E.) (Raven, New York, in the press).
Hunt, S. P. et al. Neurosci. Lett. 21, 125–130 (1981).
Tramu, G., Pillez, A. & Leonardelli, J. J. Histochem. Cytochem. 26, 322–324 (1978).
Coons, A. H. in General Cytochemical Methods (ed. Danielli, J. F.) 399–422 (Academic, New York, 1958).
Brecha, N., Karten, H. J. & Laverack, C. Proc. natn. Acad. Sci. U.S.A. 76, 3010–3014 (1979).
Karten, H. J. & Brecha, N. Nature 283, 87–88 (1980).
Korte, G. E., Reiner, A. & Karten, H. J. Neuroscience 5, 903–914 (1980).
Erichsen, J. T., Karten, H. J., Eldred, W. D. & Brecha, N. C. J. Neurosci. (in the press).
Erichsen, J. T., Reiner, A., Cabot, J. & Karten, H. J. Neurosci. Abstr. 7, 777 (1981).
Davis, B. M., Brecha, N. & Karten, H. J. Neurosci. Abstr. 6, 744 (1980).
Reiner, A., Karten, H. J. & Korte, G. E. Neurosci. Abstr. 6, 810 (1980).
Karten, H. J. & Hodos, W. A Stereotaxic Atlas of the Brain of the Pigeon (Columba livia) (Johns Hopkins Press, Baltimore, 1966).
Nicoll, R. A., Alger, B. E. & Jahr, C. E. Proc. R. Soc. 210, 133–149 (1980).
Martin, A. R. & Pilar, G. J. Physiol., Lond. 168, 443–463 (1963).
Martin, A. R. & Pilar, G. J. Physiol., Lond. 168, 464–475 (1963).
Hess, A. J. Cell Biol. 25, 1–19 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Erichsen, J., Reiner, A. & Karten, H. Co-occurrence of substance P-like and Leu-enkephalin-like immunoreactivities in neurones and fibres of avian nervous system. Nature 295, 407–410 (1982). https://doi.org/10.1038/295407a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/295407a0
- Springer Nature Limited
This article is cited by
-
Heterogeneity in calbindin-D28k expression in oxytocin-containing magnocellular neurons of the rat hypothalamus
Histochemistry (1994)
-
Localisation of substance P-like immunoreactivity in the ciliary ganglia of monkey (Macaca fascicularis) and cat: a light- and electron-microscopic study
Cell and Tissue Research (1994)
-
Immuno-electron-microscopic localization of enkephalin in the secretory granules of C cells in the chicken ultimobranchial glands
Cell & Tissue Research (1993)
-
Colocalization of neuropeptide Y (NPY)-like and FMRFamide-like immunoreactivities in the brain of the Atlantic salmon (Salmo salar)
Cell and Tissue Research (1992)
-
Co-occurrence of immunoreactive calcitonin and calcitonin gene-related peptide in neuroendocrine cells of rat lungs
Cell and Tissue Research (1991)