Abstract
The insertion sequence, IS1, which is 768 base pairs(bp) long1, is the smallest active translocatable DNA element in bacteria (for review see ref. 2), and appears as a repeated DNA sequence in some Enterobacteriaceae species3. In particular, most Shigella species contain more than 40 copies of a sequence that hybridizes to IS13. Here, we report the isolation of various repeated sequences from the Shigella dysenteriae chromosome and the DNA sequence analysis of the class, which is abut 770 bp long. The results demonstrate that there are two major types of sequence in this class; one is identical to IS1 except for 10-bp substitutions and is present in about 50 copies in the chromosome; the second sequence, 766 bp long, is repeated more than 150 times and has ∼55% homology to IS1 on the nucleotide sequence level. Because of this high degree of homology, we call these ‘iso-insertion sequences’ of IS1. Further examination of the nucleotide sequence reveals common coding frames between these sequences, suggesting the existence of two genes that are required for multiplication of these sequences.
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Ohtsubo, H., Nyman, K., Doroszkiewicz, W. et al. Multiple copies of iso-insertion sequences of IS1 in Shigella dysenteriae chromosome. Nature 292, 640–643 (1981). https://doi.org/10.1038/292640a0
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DOI: https://doi.org/10.1038/292640a0
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