Skip to main content
SpringerLink
Log in

Intervening sequences in a plant gene—comparison of the partial sequence of cDNA and genomic DNA of French bean phaseolin

  • Article
  • Published:

From Nature

View current issue Submit your manuscript

Abstract

A plant gene coding for the major storage protein (phaseolin, G1-globulin) of the French bean was isolated from a genomic library constructed in the phage vector Charon 24A. Comparison of the nucleotide sequence of part of the gene with that of the cloned messenger RNA (cDNA) revealed the presence of three intervening sequences, all beginning with GTand ending with AG. The 5′; and 3′ boundaries of intervening sequences TVS-A (88 base pairs) and IVS-B (124 base pairs) are similar to those described for animal and viral genes, but the 3′ boundary of IVS-C (129 base pairs) shows some differences. A sequence of 185 amino acids deduced from the cloned DMAs represents about 40% of a phaseolin polypeptide.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Osborne, T. B. J. Am. chem. Soc. 16, 633–764 (1894).

    Article  CAS  Google Scholar 

  2. McLeester, R. C., Hall, T. C., Sun, S. M. & Bliss, F. A. Phytochemistry 12, 85–93 (1973).

    Article  CAS  Google Scholar 

  3. Sun, S. M., McLeester, R. C., Bliss, F. A. & Hall, T. C. J. biol. Chem. 249, 2118–2121 (1974).

    CAS  PubMed  Google Scholar 

  4. Pusztai, A. & Watt, W. B. Biochim. biophys. Acta 207, 413–431 (1970).

    Article  CAS  PubMed  Google Scholar 

  5. Joubert, F. J. J. S. Afr. Chem. Inst. 10, 16–20 (1957).

    CAS  Google Scholar 

  6. Cleveland, D. W., Fischer, S. G., Kirschner, M. W. & Laemmli, U. L. J. biol. Chem. 252, 1102–1106 (1977).

    CAS  PubMed  Google Scholar 

  7. Ma, Y., Bliss, F. A. & Hall, T. C. Pl. Physiol. 66, 897–902 (1980).

    Article  CAS  Google Scholar 

  8. O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).

    CAS  PubMed  Google Scholar 

  9. Brown, J. W. S., Bliss, F. A. & Hall, T. C. Pl. Physiol. 6, 838–840 (1980).

    Article  Google Scholar 

  10. Viotti, A. et al. Eur. J. Biochem. 102, 211–222 (1979).

    Article  CAS  PubMed  Google Scholar 

  11. Park, W. D., Lewis, E. D. & Rubenstein, I. Pl. Physiol. 65, 98–106 (1980).

    Article  CAS  Google Scholar 

  12. Larkins, B. A. et al. in Genome Organization and Expression in Plants (ed. Leaver, C. J.) 203–217 (Plenum, New York, 1980).

    Book  Google Scholar 

  13. Beachy, R. N., Barton, K. A., Madison, J. T., Thompson, J. F. & Jarvis, N. in Genome Organization and Expression in Plants (ed. Leaver, C. J.) 273–281 (Plenum, New York, 1980).

    Book  Google Scholar 

  14. Moreira, M. A., Hermodson, M. A., Larkins, B. A. & Nielsen, N. C. J. biol. Chem. 254, 9921–9926 (1979).

    CAS  PubMed  Google Scholar 

  15. Sun, S. M., Mutschler, M. A., Bliss, F. A. & Hall, T. C. Pl. Physiol. 61, 918–923 (1978).

    Article  CAS  Google Scholar 

  16. Mutschler, M. A., Bliss, F. A. & Hall, T. C. Pl. Physiol. 65, 627–630 (1980).

    Article  CAS  Google Scholar 

  17. Hall, T. C. et al. in The Plant Seed (eds Rubenstein, I., Phillips, R. L., Green, C. E. & Gengenbach, B. G.) 3–25 (Academic, New York, 1979).

    Book  Google Scholar 

  18. Bliss, F. A. & Hall, T. C. Cereal Foods World 22, 106–113 (1977).

    CAS  Google Scholar 

  19. Blattner, F. R. et al. Science 202, 1279–1284 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Slightom, J. L., Blechl, A. E. & Smithies, O. Cell 21, 627–638 (1980).

    Article  CAS  PubMed  Google Scholar 

  21. Maniatis, T. et al. Cell 15, 687–701 (1978).

    Article  CAS  PubMed  Google Scholar 

  22. Blin, N. & Stafford, D. W. Nucleic. Acids. Res. 3, 2303–2308 (1976).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Hall, T. C. et al. in Genome Organization and Expression in Plants (ed. Leaver, C. J.) 259–272 (Plenum, New York, 1980).

    Book  Google Scholar 

  24. Kacian, D. L. & Myers, J. C. Proc. natn. Acad. Sci. U.S.A. 73, 2191–2195 (1976).

    Article  ADS  CAS  Google Scholar 

  25. Moore, D. D., Denniston-Thompson, K., Furth, M. E., Williams, B. G. & Blattner, F. R. Science 198, 1041–1046 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Bolivar, F. et al. Gene 2, 95–113 (1977).

    Article  CAS  PubMed  Google Scholar 

  27. Hall, T. C. et al. Proc. natn. Acad. Sci. U.S.A. 75, 3196–3200 (1978).

    Article  ADS  CAS  Google Scholar 

  28. Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).

    Article  ADS  CAS  Google Scholar 

  29. Rave, N., Crkvenjakov, R. & Boedtker, H. Nucleic Acids Res. 6, 3559–3567 (1979).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Maniatis, T., Jeffrey, A. & Kleid, D. G. Proc. natn. Acad. Sci. U.S.A. 72, 1184–1188 (1975).

    Article  ADS  CAS  Google Scholar 

  31. Paterson, B. M., Roberts, B. Z. & Kuff, E. L. Proc. natn. Acad. Sci. U.S.A. 74, 4370–4374 (1977).

    Article  ADS  CAS  Google Scholar 

  32. McKnight, G. S., Lee, D. C., Mayo, K. E. & Palmiter, R. Nucleic Acids Res. 6, 2435–2452 (1979).

    Article  PubMed  PubMed Central  Google Scholar 

  33. Wienand, U., Brüschke, C. & Feix, G. Nucleic Acids Res. 6, 2707–2715 (1979).

    Article  Google Scholar 

  34. Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–565 (1977).

    Article  ADS  CAS  Google Scholar 

  35. Tilghman, S. N. et al. Proc. natn. Acad. Sci. U.S.A. 75, 725–729 (1978).

    Article  ADS  CAS  Google Scholar 

  36. Breathnach, R. Benoist, C., O'Hare, K., Gannon, F & Chambon, P. Proc. natn. Acad. Sci. U.S.A. 75, 4853–4857 (1978).

    Article  ADS  CAS  Google Scholar 

  37. Lerner, M. R., Boyle, J. A., Mount, S. M., Wolin, S. L. & Steitz, J. A. Nature 283, 220–224 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  38. Rogers, J. & Wall, R. Proc. natn. Acad. Sci. U.S.A. 77, 1877–1879 (1980).

    Article  ADS  CAS  Google Scholar 

  39. Sun, S. M. thesis, Univ. Wisconsin, Madison (1974).

  40. Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

    Article  CAS  PubMed  Google Scholar 

  41. Buchbinder, B. U. thesis, Univ. Wisconsin, Madison (1980).

  42. Brakke, M. K. & Van Pelt, N. Analyt. Biochem. 38, 56–64 (1970).

    Article  CAS  PubMed  Google Scholar 

  43. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  44. Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).

    Article  CAS  PubMed  Google Scholar 

  45. Fyrberg, E. A., Kindle, K. L., Davidson, N. & Sodja, A. Cell 19, 365–378 (1980).

    Article  CAS  PubMed  Google Scholar 

  46. IUPAC-IUB Commission Biochem. J. 113, 1–4 (1969).

Download references

Author information

Author notes

  1. S. M. Sun

    Present address: Atlantic Richfield Co., 6905 Sierra Court, PO Box 2600, Dublin, California, 94566

  2. J. L. Slightom

    Present address: Departments of Chemistry and Biochemistry, Southern Illinois University, Carbondale, Illinois, 62901

Authors and Affiliations

  1. Departments of Horticulture and Genetics, University of Wisconsin, Madison, Wisconsin, 53706

    J. L. Slightom

Authors
  1. S. M. Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. L. Slightom
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. C. Hall
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sun, S., Slightom, J. & Hall, T. Intervening sequences in a plant gene—comparison of the partial sequence of cDNA and genomic DNA of French bean phaseolin. Nature 289, 37–41 (1981). https://doi.org/10.1038/289037a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/289037a0

  • Springer Nature Limited

This article is cited by

Search

Navigation