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Isolation of a gene from Drosophila by complementation in yeast

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Abstract

Transformation of mutant yeast cells by cloned genomic DNA from a higher eukaryote has made it possible to isolate a Drosophila DNA sequence that complements a yeast adenine-8 mutation. A 0.8-kilobase poly(A)-containing RNA is transcribed from the cloned Drosophila segment in transformed yeast cells and can account for functional expression of the gene.

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Henikoff, S., Tatchell, K., Hall, B. et al. Isolation of a gene from Drosophila by complementation in yeast. Nature 289, 33–37 (1981). https://doi.org/10.1038/289033a0

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  • DOI: https://doi.org/10.1038/289033a0

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