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Production of human hybridomas secreting antibodies to measles virus

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Abstract

Monoclonal antibodies against a variety of antigens can be produced using techinques of somatic cell hybridization between cells of rodent myeloma lines and B cells derived from animals immunized against a given antigen1,2. However, because the monoclonal antibodies secreted by these hybridomas are of rodent origin, their use in human immunotherapy is limited. Thus the production of B-cell hybrids that secrete human monoclonal antibodies may be of considerable value. We have hybridized a hypoxanthine phosphoribosyl transferase (HPRT)-deficient human B-cell line derived from a patient suffering from multiple myeloma with peripheral lymphocytes obtained from a patient with subacute sclerosing panencephalitis (SSPE). These hybridomas were found to secrete human IgM specific for measles virus nucleocapsids.

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Authors and Affiliations

  1. The Wistar Institute of Anatomy and Biology, 36th Street at Spruce, Philadelphia, Pennsylvania, 19104

    Carlo M. Croce, Alban Linnenbach, Zenon Steplewski & Hilary Koprowski

  2. Rockefeller University, New York, New York, 10021

    William Hall

Authors
  1. Carlo M. Croce
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  2. Alban Linnenbach
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  3. William Hall
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  4. Zenon Steplewski
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  5. Hilary Koprowski
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Croce, C., Linnenbach, A., Hall, W. et al. Production of human hybridomas secreting antibodies to measles virus. Nature 288, 488–489 (1980). https://doi.org/10.1038/288488a0

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  • DOI: https://doi.org/10.1038/288488a0

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