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Synthesis in E. coli of a polypeptide with human leukocyte interferon activity

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Abstract

Double-stranded cDNA prepared from the 12S fraction of poly (A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones.

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Author notes

  1. Kari Cantell: Central Public Health Laboratory, SF 00280 Helsinki, Finland

Authors and Affiliations

  1. Institut für Molekularbiologie I, Universität Zürich, 8093, Zürich, Switzerland

    Shigekazu Nagata, Hideharu Taira, Alan Hall, Lorraine Johnsrud, Michel Streuli, Josef Ecsödi, Werner Boll, Kari Cantell & Charles Weissmann

Authors
  1. Shigekazu Nagata
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  2. Hideharu Taira
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  3. Alan Hall
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  4. Lorraine Johnsrud
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  5. Michel Streuli
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  6. Josef Ecsödi
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  7. Werner Boll
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  8. Kari Cantell
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  9. Charles Weissmann
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Nagata, S., Taira, H., Hall, A. et al. Synthesis in E. coli of a polypeptide with human leukocyte interferon activity. Nature 284, 316–320 (1980). https://doi.org/10.1038/284316a0

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  • DOI: https://doi.org/10.1038/284316a0

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