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Reverse Genetics of Hepatitis C Virus Using an RNA Polymerase I-Mediated Transcription

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Reverse Genetics of RNA Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2733))

Abstract

The reverse genetics system commonly used for the production of hepatitis C virus (HCV), which is a major causative agent of liver diseases, involves introduction of the viral genomic RNA synthesized in vitro into human hepatoma cells by electroporation. As an alternative methodology, we describe a cell culture system based on transfection with an expression plasmid containing a full-length HCV cDNA clone flanked by RNA polymerase I promoter and terminator sequences to generate infectious virus particles from transfected cells.

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Acknowledgments

We thank Dr. Mami Matsuda for her constructive suggestions. This work was supported by the Japan Agency for Medical Research and Development (AMED), under Grant Numbers JP21fk0210053, JP22fk0210086, JP22fk0210090, JP22fk0210066, JP22fk0210110, and JP22fk0210109, and by MEXT KAKENHI Grant Number JP20K08852.

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Authors and Affiliations

  1. Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan

    Ryosuke Suzuki

  2. Department of Microbiology and Immunology, Hamamatsu University School of Medicine, Hamamatsu, Japan

    Tetsuro Suzuki

Authors
  1. Ryosuke Suzuki
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  2. Tetsuro Suzuki
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Corresponding author

Correspondence to Tetsuro Suzuki .

Editor information

Editors and Affiliations

  1. College of Veterinary Medicine, University of Georgia, Athens, GA, USA

    Daniel R. Pérez

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Suzuki, R., Suzuki, T. (2024). Reverse Genetics of Hepatitis C Virus Using an RNA Polymerase I-Mediated Transcription. In: Pérez, D.R. (eds) Reverse Genetics of RNA Viruses. Methods in Molecular Biology, vol 2733. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3533-9_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3533-9_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3532-2

  • Online ISBN: 978-1-0716-3533-9

  • eBook Packages: Springer Protocols

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