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Plasmid incompatibility: cloning analysis of an inc FII determinant of R6-5

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Abstract

SalI and PstI restriction endonuclease-generated DNA fragments that specify an FII-type incompatibility function (incFII) of the low copy number antibiotic resistance plasmid R6-5 have been cloned in the high copy number pBR322 plasmid vector. A 1-kilobase DNA sequence that contains this incFII determinant has been identified and is shown to have coordinates of 95.5 and 96.5 kilobases on the R6-5 plasmid physical map. Expression of incompatibility by the cloned PstI fragment depends on its orientation within the vector molecule. The behaviour of pBR322-incFII hybrid plasmids suggests that plasmid replication control is not the only mechanism that can cause incompatibility between two plasmids.

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Authors and Affiliations

  1. Max-Planck-Institut für Molekulare Genetik, 1000 Berlin 33 (Dahlem), Ihnestrasse 63-73, FRG

    Kenneth N. Timmis, Isabel Andrés & Patrick M. Slocombe

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  1. Kenneth N. Timmis
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  2. Isabel Andrés
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  3. Patrick M. Slocombe
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Timmis, K., Andrés, I. & Slocombe, P. Plasmid incompatibility: cloning analysis of an inc FII determinant of R6-5. Nature 273, 27–32 (1978). https://doi.org/10.1038/273027a0

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